Artificial Cultivation of Ganoderma lucidum (Reishi Medicinal Mushroom) Using Different Sawdusts as Substrates

Abstract

Ganoderma lucidum is a kind of medicinal mushroom possessing anti-tumor, anti-inflammatory, immune-modulatory, antioxidant and other biological traits which render it to be used as medicinal herbs to combat against variety of diseases. Present study was designed to implement a suitable method for artificial cultivation of G. lucidum in polypropylene bags with variety of cheap and readily available substrates. Sawdusts of five woods (Swietenia mahagoni, Dipterocarpus turbinatus, Tectona grandis, Gmelina arborea and Michelia champaca) were used as substrates and each was supplemented with calcium carbonate (CaCO 3) and either rice or wheat bran for cultivation. T. grandis, G. arborea and M. champaca were not found to provoke the further extension of mycelial growth and hence the growth was stunted. On the contrary, S. mahagoni and D. turbinatus were noticed to impart comparatively good yield with biological efficiency. Wheat bran was found to be more efficient as supplement than rice bran. However, S. mahagoni supplemented with wheat bran provided the best yield of mushroom among the substrates which took 6 days, 33 days and 60 days for the mycelial growth, primordial formation and harvesting, consecutively with the subsequent yields of 235.2 g/kg and biological efficiency of 7.6%.

A Comprehensive and Comparative Study of Wolfiporia extensa Cultivation Regions by Fourier Transform Infrared Spectroscopy and Ultra-Fast Liquid Chromatography

“…In general, the different methods used in cultivated mushrooms do have effects on the morphology of fruiting bodies, yield percentage, functional, organoleptic and chemical properties as well as the quality of mushrooms [52]. For example, Roy et al [53] analyzed the G . lucidum cultivated with the sawdusts of five woods and found the difference in yield among samples.…”

Morphological Characteristics, Yield Performance, and Medicinal Value of Some Lingzhi Mushroom (Ganoderma lucidum) Strains Cultivated in Tam Dao, Vietnam

“…In another experiment, G. lucidum cultivated on paddy straw supplemented with wheat bran showed the maximum yield (82.5g) and biological efficiency (27.5%) (Jandaik et al, 2013). Furthermore, Swietenia mahagoni supplemented with wheat bran was observed as the optimal substrate for the cultivation of G. lucidum with a yield of 235.2 g kg -1 and biological efficiency of 7.6% (Roy et al, 2015). In comparison to previously published data, the strains GA1, GA2, and GA3 showed high yields and, therefore, could be considered as promising candidates for commercial cultivation.…”

“…Owing to scarcity in nature, Lingzhi mushrooms are artificially cultivated in solid culture to meet demands for medicinal herbs and international markets, especially in tropical Asian countries (Boh et al, 2007;Gurung et al, 2012;Roy et al, 2015;Ninluam et al, 2016;Chang & Buswell, 2018). Grain, sawdust, wood logs, cork residues, sunflowers, and seed hulls are commonly utilized as the main substrates and nutritional sources for traditional artificial cultivation of G. lucidum (Riu et al,1997;Chang & Buswell, 1999;Gonzalez-Matute et al, 2002;Wasser et al, 2005;Boh et al, 2007).…”

Effect of Strain, Wood Substrate and Cold Treatment on the Yield and β-Glucan Content of Ganoderma lucidum Fruiting Bodies

“…G. lucidum has been cultivated in a wide variety of substrates including sawdust from Acacia sp., Alnus sp., Carpinus sp., Dalbergia sp., Dipterocarpus sp., Fagus sp., Pinus sp., Populus sp., Quercus sp. and Swietenia sp., among others [ 6 , 7 , 8 , 14 , 15 , 16 , 17 ]. Ganoderma contains several compounds that define the bioactive and pharmacological properties of the fungus such as antiviral [ 18 ], immunomodulatory, antitumor, antioxidant, hepatoprotective or antihypertensive activities [ 19 ].…”