Are aqueous solutions of choline-based ionic liquid biocompatible cross-linkers for collagen?

Tarannum, Aafiya; Lobo, Nitin P.; Rao, Jonnalagadda Raghava; Fathima, Nishter Nishad

doi:10.1016/j.molliq.2020.112654

Cited by 9 publications

(12 citation statements)

References 53 publications

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“…ILs, being less toxic cosolvents, are now employed as chemical cross-linkers for biomolecules. The development of biocompatible cross-linkers for biomaterials is a prerequisite as it facilitates the stability of the biomolecules, like proteins. − In this context, MacFarlane and co-workers pioneered the introduction of Chn ILs as chemical cross-linkers for collagen. They successfully synthesized cholinium amino acid based ILs including cholinium lactate [Chn][Lac], cholinium levulinate [Chn][Lev], and cholinium tartarate [Chn][Tar] and used them as a replacement for the common cross-linking agent glutaraldehyde (GA) to reduce the toxicity of the collagen-based biomaterials.…”

Section: Recent Advances In Exploring Cholinium-based Ionic Liquids F...mentioning

confidence: 99%

“…In another pioneering work, Fathima and co-workers investigated whether cholinium acetate [Chn][Ac], [Chn][Cit], and cholinium sulfate [Chn][Sul] act as biocompatible cross-linkers for collagen. In this regard, a combination of transverse NMR relaxation and impedance measurements was employed along with conformational and thermal stability analysis.…”

Section: Recent Advances In Exploring Cholinium-based Ionic Liquids F...mentioning

confidence: 99%

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Contemporary Advancement of Cholinium-Based Ionic Liquids for Protein Stability and Long-Term Storage: Past, Present, and Future Outlook

Sindhu

Kumar

Venkatesu

2022

ACS Sustainable Chem. Eng.

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In the past two decades, ionic liquids (ILs) have been acknowledged as potentially attractive “green” and “designer” solvents since their broader chemical space and the tunable nature of the diverse ions can finely modulate their physiochemical properties while enabling task-specific optimization. They have found numerous applications in fields ranging from enzymatic reactions to protein preservation, which focused on the development of various types of ILs and their application for providing protein stability in vitro. Among various families of ILs, some of them have serious limitations similar to organic solvents; they cause environmental toxicity as they are non-biodegradable. In other words, some of the ILs impart stress to biomolecules and ultimately denature the protein. In this regard, the need for biocompatible ILs has come to the light and cholinium-based ILs (Chn ILs) have proved themselves as the most promising ILs to support the structure of biomolecules. The family of Chn ILs has only taken growth in recent years. Despite the numerous studies, more exhaustive research in the field of Chn ILs and biomolecules still needs acceleration. Herein, we review the strategies and current progress on Chn ILs for protein and enzyme-based applications keeping in mind all crucial past and present research outcomes. Furthermore, we elucidate an overview of the various ways to enhance enzymatic activity, structural stability, and long-term storage of proteins in the presence of Chn ILs. We believe these insights will be fruitful for designing various processes based on ILs in the diverse field of biotechnology and ILs will serve as novel solvents for protein stability and enzymatic reactions, maintaining their utility in industrial and biomedical based applications. The huge varieties of biobased Chn ILs hold the promise of recent advances and developments for the correct selection of long-term storage of enzymes.

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Section: Recent Advances In Exploring Cholinium-based Ionic Liquids F...mentioning

confidence: 99%

Section: Recent Advances In Exploring Cholinium-based Ionic Liquids F...mentioning

confidence: 99%

Contemporary Advancement of Cholinium-Based Ionic Liquids for Protein Stability and Long-Term Storage: Past, Present, and Future Outlook

Sindhu

Kumar

Venkatesu

2022

ACS Sustainable Chem. Eng.

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“…The concentrations of ILs were varied from 12.5 to 100 mM. Before all the measurements, the samples were equilibrated for 30 provided in Table S1 in Supporting Information. To study the effect of pH of IL solution on protein, the solutions were prepared in distilled deionized and the pH was adjusted to 6.5 using dilute NaOH.…”

Section: ■ Experimental Sectionmentioning

confidence: 99%

“…The authors observed that cholinium chloride [Chn][Cl] and dilute cholinium hexanoate ([Chn][Hex]) and cholinium aspartate ([Chn][Asp]) were successful in maintaining the conformational stability of avidin. Moreover, Tarannum et al have examined the effect of an aqueous solution of cholinium-based ILs on collagen. The data showed an increase in the melting and denaturation temperature of collagen as a function of the concentration of ILs thereby indicating stability and compactness in the structure of collagen .…”

Section: Introductionmentioning

confidence: 99%

“…Moreover, Tarannum et al have examined the effect of an aqueous solution of cholinium-based ILs on collagen. The data showed an increase in the melting and denaturation temperature of collagen as a function of the concentration of ILs thereby indicating stability and compactness in the structure of collagen . Interestingly, the detailed structure, thermal stability and the activity of the enzymes in multicationic cholinium-based ILs are all presently poorly understood.…”

Section: Introductionmentioning

confidence: 99%

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Assessing the Compatibility of Mono-, Di-, and Tri-Cholinium Citrate Ionic Liquids for the Stability and Activity of α-Chymotrypsin

Sindhu

Bisht

Bahadur

et al. 2021

ACS Sustainable Chem. Eng.

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In view of the global environmental and economic challenges of conventional organic solvents, most of the ionic liquids (ILs) have proved themselves as a “greener” group of cosolvents, especially for enzymes. However, there is a lack of fundamental knowledge on the greener aspects of various multicationic IL systems on biomolecules. In this regard, we have synthesized the monocholinium citrate ([Chn][Cit]), dicholinium citrate ([Chn]2[Cit]) and tricholinium citrate ([Chn]3[Cit]) ILs and employed them to explore their suitability for α-chymotrypsin (α-CT). This study specifically addresses the effect of increased cationic ratio of IL on stability and activity of α-CT with the help of various spectroscopic techniques. Various spectroscopic analysis showed that there is improved structural stability and activity of α-CT as the cationic ratio of IL increase. We found better structural stability of α-CT in [Chn]3[Cit] and [Chn]2[Cit] as compared to that in [Chn][Cit]. Interestingly, temperature-dependent fluorescence spectroscopy results explicitly elucidate that transition temperature (T m) of α-CT enhances by 6 °C in the presence of [Chn]2[Cit] when compared to T m of α-CT in buffer solution. Ultimately, these results establish that through an increase in the molar ratio of aqueous cholinium cation in [Chn][Cit] intensifies the capability of ILs to stabilize the α-CT structure. Our results suggested that multicationic IL system sensitivity to cosolvent exposure may be utlized in the future as a new approach to explore new biocompatibility toward proven stability studies.

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Bioionic Liquids: Enabling a Paradigm Shift Toward Advanced and Smart Biomedical Applications

Kanjilal

Zhu

Krishnadoss

et al. 2023

Advanced Intelligent Systems

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Figure 11. Application of cholinium BILs in encapsulation and targeted delivery. Usage of cholinium BILs enhances the biocompatibility of aggregated structures lowering the propensity of attack and metabolic breakdown by the immune system prior to reaching the targeted delivery spot. a) Encapsulation and delivery of nucleic acids using DNA amphiphiles, self-assembled with lysosomal phospholipid base choline BIL. Reproduced with permission. [133] Copyright 2020, John Wiley and Sons. b) Ester-functionalized SAILs based on nicotine BILs forming aggregates. Reproduced with permission. [130] Copyright 2017, Elsevier. c) Cholinium and other ammonium IL-based drug nanocarriers where chloride anions of the polymer chain ion are exchanged with the pharmaceutical anions for improved drug delivery. Reproduced with permission. [129] Copyright 2021, MDPI.

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Are aqueous solutions of choline-based ionic liquid biocompatible cross-linkers for collagen?

Cited by 9 publications

References 53 publications

Contemporary Advancement of Cholinium-Based Ionic Liquids for Protein Stability and Long-Term Storage: Past, Present, and Future Outlook

Contemporary Advancement of Cholinium-Based Ionic Liquids for Protein Stability and Long-Term Storage: Past, Present, and Future Outlook

Assessing the Compatibility of Mono-, Di-, and Tri-Cholinium Citrate Ionic Liquids for the Stability and Activity of α-Chymotrypsin

Bioionic Liquids: Enabling a Paradigm Shift Toward Advanced and Smart Biomedical Applications

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