Abstract:In
the past two decades, ionic liquids (ILs) have been acknowledged
as potentially attractive “green” and “designer”
solvents since their broader chemical space and the tunable nature
of the diverse ions can finely modulate their physiochemical properties
while enabling task-specific optimization. They have found numerous
applications in fields ranging from enzymatic reactions to protein
preservation, which focused on the development of various types of
ILs and their application for providing protein stability… Show more
“…Both DESs were synthesized by using the standard protocol. 33,34 The process involved a hydrogen bond acceptor, [Ch]Cl, and a hydrogen bond donor, urea/Gly, as the starting materials. DES 1 was prepared by mixing [Ch]Cl and urea in a molar ratio of 1 : 2.…”
Section: Synthesis Of Dessmentioning
confidence: 99%
“…30 Moreover, tonnes of [Ch]Cl are produced annually to be used as an animal feed supplement, and therefore, it is not expensive. [31][32][33] The characteristics of [Ch]Cl-based DESs could be regulated by changing the type of HBD and the molar ratio of HBD to HBA. As HBDs, we have chosen Gly and urea.…”
Recently, a great focus on deep eutectic solvents (DESs) has been achieved, due to their eco-friendly, less toxic, and resource-efficient nature. Therefore, in this work we have investigated the structural...
“…Both DESs were synthesized by using the standard protocol. 33,34 The process involved a hydrogen bond acceptor, [Ch]Cl, and a hydrogen bond donor, urea/Gly, as the starting materials. DES 1 was prepared by mixing [Ch]Cl and urea in a molar ratio of 1 : 2.…”
Section: Synthesis Of Dessmentioning
confidence: 99%
“…30 Moreover, tonnes of [Ch]Cl are produced annually to be used as an animal feed supplement, and therefore, it is not expensive. [31][32][33] The characteristics of [Ch]Cl-based DESs could be regulated by changing the type of HBD and the molar ratio of HBD to HBA. As HBDs, we have chosen Gly and urea.…”
Recently, a great focus on deep eutectic solvents (DESs) has been achieved, due to their eco-friendly, less toxic, and resource-efficient nature. Therefore, in this work we have investigated the structural...
“…67 Although there have been significant inroads in understanding protein−IL interactions, the question remains whether they can stabilize the protein−NP interface, which is critical for biomedicine and drug delivery formulations. 68,69 In this milieu, we have used choline cation-based ILs with two different anions as the negative counterparts. Choline was the preferred choice for the cationic counterpart of the IL, as it is biocompatible.…”
The emergence of nanoparticles in biomedical applications has made their interactions with proteins inevitable. Nanoparticles conjugated with proteins and peptide-based constructs form an integral part of nanotherapeutics and have recently shown promise in treating a myriad of diseases. The proper functioning of proteins is critical to achieve their biological functions. However, interface issues result in the denaturation of proteins, and the loss of orientation and steric hindrance can adversely affect the function of the conjugate. Furthermore, surface-induced denaturation also triggers protein aggregation, resulting in amyloid-like species. Understanding the mechanistic underpinnings of protein−nanoparticle interactions and controlling their interfacial characteristics are critical and challenging due to the complex nature of the conjugates. In this milieu, we demonstrate that ionic liquids can be suitable candidates for stabilizing protein−nanoparticle interactions by virtue of their excellent protein-preserving properties. We also probe the previously unexplored mechanism of ion-mediated stabilization of the protein molecules on the nanoparticle surface. The protein−nanoparticle conjugates consist of lysozyme and choline-based ionic liquids characterized by optical and electron microscopy techniques combined with surface-sensitive plasmon-enhanced Raman spectroscopy. Furthermore, atomistic molecular dynamics simulations of the conjugates delineate interfacial interactions of the protein molecules and the modulation by the ions, particularly the conformational changes and the dynamic correlation when the protein and specific ionic liquid molecules are adsorbed on the nanoparticle surface. The combined experimental and computational studies showed the synergistic behavior of the ions of the ionic liquids, specifically the orientation and coverage of the anions aided by the cations to control the surface interactions and hence the overall protein stability. These studies pave the way for using ionic liquids, particularly their biocompatible counterparts in nanoparticle-based complexes, as stabilizing agents for biomedical applications.
“…Although the interaction between proteins is essential for life, an abnormal increase in protein–protein interactions can lead to the unwanted formation of protein aggregates, which play major roles in diseases such as Alzheimer’s, type 2 diabetes, and spongiform encephalopathies [ 4 ]. This inherent structural and chemical instability, associated with short half-lives when subjected to physical and chemical stress, limits the use of proteins as therapeutic agents [ 5 ]. However, since proteins are stabilized by the equilibrium between intramolecular interactions and interactions with the solvent environment, the employment of biocompatible cosolvents can provide an alternative strategy to preserve their stability [ 5 ].…”
Section: Introductionmentioning
confidence: 99%
“…This inherent structural and chemical instability, associated with short half-lives when subjected to physical and chemical stress, limits the use of proteins as therapeutic agents [ 5 ]. However, since proteins are stabilized by the equilibrium between intramolecular interactions and interactions with the solvent environment, the employment of biocompatible cosolvents can provide an alternative strategy to preserve their stability [ 5 ].…”
Proteins are inherently unstable, which limits their use as therapeutic agents. However, the use of biocompatible cosolvents or surfactants can help to circumvent this problem through the stabilization of intramolecular and solvent-mediated interactions. Ionic liquids (ILs) have been known to act as cosolvents or surface-active compounds. In the presence of proteins, ILs can have a beneficial effect on their refolding, shelf life, stability, and enzymatic activities. In the work described herein, we used small-angle X-ray scattering (SAXS) to monitor the aggregation of different concentrations of ILs with protein models, lysozyme (Lys) and bovine serum albumin (BSA), and fluorescence microscopy to assess micelle formation of fluorinated ILs (FILs) with Lys. Furthermore, coarse-grained molecular dynamics (CG-MD) simulations provided a better understanding of Lys–FIL interactions. The results showed that the proteins maintain their globular structures in the presence of FILs, with signs of partial unfolding for Lys and compaction for BSA with increased flexibility at higher FIL concentrations. Lys was encapsulated by FIL, thus reinforcing the potential of ILs to be used in the formulation of protein-based pharmaceuticals.
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