Aqueous extract of Plinia edulis leaves: Antioxidant activity and cytotoxicity to human breast cancer MCF-7 cell line

Carvalho, André; Ishikawa, Tati; Gouvêa, Cibele Marli Cação Paiva

doi:10.1016/j.sajb.2012.03.010

Cited by 19 publications

(14 citation statements)

References 24 publications

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“…However, these secondary metabolites exert their anticancer activity through different mechanisms. Carvalho et al [20] reported that plant extracts can decrease cell proteins, alter cell morphology, and fragment nucleosomal DNA of cancer cells, resulting in cell apoptosis. Therefore cardiac glycosides may induce immunogenic cancer cell death.…”

Section: Discussionmentioning

confidence: 99%

Phytochemical Screening and Cytotoxicity of Crude Extracts of <i>Vatica diospyroides</i> Symington Type LS

Srisawat¹,

Chumkaew²,

Heed-Chim³

et al. 2013

Trop. J. Pharm Res

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Section: Discussionmentioning

confidence: 99%

Phytochemical Screening and Cytotoxicity of Crude Extracts of <i>Vatica diospyroides</i> Symington Type LS

Srisawat¹,

Chumkaew²,

Heed-Chim³

et al. 2013

Trop. J. Pharm Res

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“…Prior to analysis, cells were once more rinsed with PBS and stained by standard HE staining operations. Cells morphology were determined with an inverted light microscope, and comparisons were conducted between control and treated groups (Lin et al, 2010;Yathindranath et al, 2011;Berrington & Lall, 2012;Carvalho et al, 2012).…”

Section: He Staining Analysismentioning

confidence: 99%

“…The most promptly investigated consequence, following cell exposure to toxics, is the change of appearance in the cell-layer and/or cell-shape in monolayer culture circumstances (Carvalho et al, 2012). ALA-NLC was employed to explore the mechanism of action by monitoring the alteration in cell appearance after the exposure of HaCaT cells to the sample and comparing them with culture medium controls under light microscopy.…”

Section: Cell Morphological Analysismentioning

confidence: 99%

“…The possible poisonous effects of a substance are intensely relied on its concentration and biochemical surroundings, which should be comprehensively apprehended before any preventive or medicinal utilization is premeditated (Carvalho et al, 2012). The investigation on the security of ALA-NLC for dietary, therapeutic and cosmetic application was presently in progress in our laboratory, comprising in vivo measurements.…”

Section: Morphological Observation Of the Hacat Cell Nucleus After Exmentioning

confidence: 99%

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Alpha-lipoic acid-loaded nanostructured lipid carrier: sustained release and biocompatibility to HaCaT cellsin vitro

Wang

Xia²

2013

Drug Delivery

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ALA-loaded nanostructured lipid carrier (ALA-NLC) was designed to improve physicochemical stability and water solubility, and promote sustained release of ALA as well as determine the biocompatibility of ALA-NLC. The ALA-NLC manufactured using hot high-pressure homogenization technique was investigated in terms of size, zeta potential, FTIR analysis and release behavior. In vitro cytotoxicity and biocompatibility were determined by incubating with HaCaT cells using the MTT assay, HE staining and Hoechst 33342 staining. Cell behavior and cellular division of HaCaT cells untreated and treated by ALA-NLC were investigated in real-time images gathered using time-lapse imaging system. The release investigation illustrated that only 6.9% of ALA released in 30 min from ALA-NLC formation, whereas it was 30.3% in free ALA system. ALA-NLC possessed a satisfactory release behavior of sustained release up to 72 h. It showed that ALA-NLC did not exert hazardous effect on HaCaT cells up to 81.9 mg/L without morphological alterations, revealing a satisfactory biocompatibility. Evidence was provided from time-lapse imaging system that cell behavior and cellular division of ALA-NLC treated HaCaT cells were in accordance with the control. These results of this investigation demonstrated that NLC encapsulated ALA formation (ALA-NLC) can improve stability, solubility and release of ALA; ALA-NLC was biocompatible to HaCaT cells.

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“…Breast cancer is the third most common cause of cancer-related deaths, and is the second most common cancer in women worldwide (Engel et al, 2011;Carvalho et al, 2012). Breast cancer-related mortality rates continue to increase every year.…”

Section: Introductionmentioning

confidence: 99%

Cytotoxic and immunomodulatory effects of Ebenus boissieri Barbey on breast cancer cells

et al. 2016

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ABSTRACT. We aimed to determine the cytotoxic and immunomodulatory effects of hydroalcoholic extracts of the roots and aerial parts of Ebenus boissieri (EB) on breast cancer MDA-MB231 cells and the non-cancerous human embryonic kidney cell line, 293T. Cell viability was determined by MTT assay, trypan blue exclusion, and Live/Dead Viability/Cytotoxicity assay. Apoptosis was evaluated by measuring the activity of caspase-2, 3, 6, 8, and 9. Tumor necrosis factor (TNF)-α and interferon (IFN)-g release was assayed by ELISA, and protein expression of caspase-3, TNF-α, and IFN-g was determined by western blot. The results of this study revealed that MDA-MB231 cell viability was reduced in a dose-dependent manner by the aerial and root extract of EB at 72 h with a half-maximal inhibitory concentration (IC 50 ) of 41.1 ± 2.76 and 65 ± 1.09 µg/mL, respectively. In contrast, neither the aerial nor the root extracts of this plant inhibited the proliferation of 293T cells at doses up to 1000 µg/mL. There was a time-dependent increase in caspase activity, especially caspase-3 and caspase-9. The levels of TNF-α and IFN-g significantly increased in MDA-MB231 cells treated with aerial extract. In conclusion, the extracts of EB induced apoptosis in breast cancer cells by altering the levels of caspases, TNF-α, and IFN-g. The components and precise modes of action of EB have not yet been determined. However, potential antitumor and immunomodulatory activity was observed along with selectivity against cancer cells in vitro, suggesting that hydroalcoholic extracts of this plant are worthy of additional study.

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Aqueous extract of Plinia edulis leaves: Antioxidant activity and cytotoxicity to human breast cancer MCF-7 cell line

Cited by 19 publications

References 24 publications

Phytochemical Screening and Cytotoxicity of Crude Extracts of <i>Vatica diospyroides</i> Symington Type LS

Phytochemical Screening and Cytotoxicity of Crude Extracts of <i>Vatica diospyroides</i> Symington Type LS

Alpha-lipoic acid-loaded nanostructured lipid carrier: sustained release and biocompatibility to HaCaT cellsin vitro

Cytotoxic and immunomodulatory effects of Ebenus boissieri Barbey on breast cancer cells

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