Cytotoxic and immunomodulatory effects of Ebenus boissieri Barbey on breast cancer cells

Abstract

ABSTRACT. We aimed to determine the cytotoxic and immunomodulatory effects of hydroalcoholic extracts of the roots and aerial parts of Ebenus boissieri (EB) on breast cancer MDA-MB231 cells and the non-cancerous human embryonic kidney cell line, 293T. Cell viability was determined by MTT assay, trypan blue exclusion, and Live/Dead Viability/Cytotoxicity assay. Apoptosis was evaluated by measuring the activity of caspase-2, 3, 6, 8, and 9. Tumor necrosis factor (TNF)-α and interferon (IFN)-g release was assayed by ELISA, and protein expression of caspase-3, TNF-α, and IFN-g was determined by western blot. The results of this study revealed that MDA-MB231 cell viability was reduced in a dose-dependent manner by the aerial and root extract of EB at 72 h with a half-maximal inhibitory concentration (IC 50 ) of 41.1 ± 2.76 and 65 ± 1.09 µg/mL, respectively. In contrast, neither the aerial nor the root extracts of this plant inhibited the proliferation of 293T cells at doses up to 1000 µg/mL. There was a time-dependent increase in caspase activity, especially caspase-3 and caspase-9. The levels of TNF-α and IFN-g significantly increased in MDA-MB231 cells treated with aerial extract. In conclusion, the extracts of EB induced apoptosis in breast cancer cells by altering the levels of caspases, TNF-α, and IFN-g. The components and precise modes of action of EB have not yet been determined. However, potential antitumor and immunomodulatory activity was observed along with selectivity against cancer cells in vitro, suggesting that hydroalcoholic extracts of this plant are worthy of additional study.

Synthesis, characterization, solubilization, cytotoxicity and antioxidant activity of aminomethylated dihydroquercetin

“…30 MTT assay has been widely used to determine the cytotoxic effect of natural compounds. 31 In the present study, the cytotoxic effects of various concentrations of DHQ, DHQA and positive controls on HEK-293T and Hela cells were assessed using the MTT assay.…”

Taxonomic Comparison, Antioxidant and Antibacterial Activities of Three Ebenus pinnata Ait. ecotypes (Fabaceae) from Algeria

“…have proven to be with significant antioxidant and antibacterial activities due to various chemical compounds, especially rutin, the dominant component in Ebenus species [21]. Analyses of roots and aerial parts extracts of E. boissieri Barbey have revealed immunomodulatory and antitumor activity inducing apoptosis in breast cancer cells [22], caspase-mediated apoptosis on the cervical cancer cell line Hela [23], and cytotoxic and apoptotic effects on the human lung cancer cell line A549 [24]. In addition to its osteoprotective role, E. cretica is very rich in flavonoids and isoflavones, such as formononetin, maesopsin glucoside (aurone), and other compounds [25].…”

A novel antitumor compound nobiliside D isolated from sea cucumber (Holothuria nobilis Selenka)

“…3). Additionally, dead cells were identified in all cell line cultures, as previously described (12). The percentages of dead cells were approximately 0.6, 0.9, 2.6.…”