Aptamers as a replacement for antibodies in enzyme-linked immunosorbent assay

Toh, Saw Yi; Citartan, Marimuthu; Gopinath, Subash C.B.; Tang, Thean‐Hock

doi:10.1016/j.bios.2014.09.026

Cited by 475 publications

(263 citation statements)

References 115 publications

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“…Aptamers can be considered as artificial alternatives to antibodies, produced via chemical synthesis (Toh et al, 2015). Their in vitro selection process enables the generation of different aptamers selectively binding a huge variety of different target molecules, even toxic or low immunogenic ones (Mairal et al, 2008).…”

Section: Initial Results: Aptamer-based Detection Of C-reactive Protementioning

confidence: 99%

Design and evaluation of split-ring resonators for aptamer-based biosensors

Reinecke

Walter

Kobelt

et al. 2018

J. Sens. Sens. Syst.

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Abstract. Split-ring resonators are electrical circuits, which enable highly sensitive readout of split capacity changes via a measurement of the shift in the resonance frequency. Thus, functionalization of the split allows the development of biosensors, where selective molecular binding causes a change in permittivity and therefore a change in split capacity. In this work, we present a novel approach using transmission line theory to describe the dependency between permittivity of the sample and resonance frequency. This theory allows the identification of all relevant parameters of a split-ring resonator and thus a target-oriented optimization process. Hereby all setup optimizations are verified with measurements. Subsequently, the split of a resonator is functionalized with aptamers and the sensor response is investigated. This preliminary experiment shows that introducing the target protein results in a shift in the resonance frequency caused by a permittivity change due to aptamer-mediated protein binding, which allows selective detection of the target protein.

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Section: Initial Results: Aptamer-based Detection Of C-reactive Protementioning

confidence: 99%

Design and evaluation of split-ring resonators for aptamer-based biosensors

Reinecke

Walter

Kobelt

et al. 2018

J. Sens. Sens. Syst.

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show abstract

“…29 Aptamers have the advantages of being highly specific, relatively small in size, and easily synthesized, which could be regarded as a chemical equivalent of antibodies. 30,31 In this study, we adopted a truncated aptamer A10-3.2 with 39 nucleotides for its simplicity of chemical synthesis and enhanced specificity. It is about 55% of the full A10 in size.…”

Section: Discussionmentioning

confidence: 99%

A specific aptamer-cell penetrating peptides complex delivered siRNA efficiently and suppressed prostate tumor growthin vivo

Yanjun

Liu

et al. 2016

Cancer Biology & Therapy

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Specific and efficient delivery of siRNA into intended tumor cells remains as a challenge, even though RNAi has been exploited as a new strategy for prostate cancer therapy. This work aims to address both specificity and efficiency of SURVIVIN-siRNA delivery by constructing a therapeutic complex using combinatorial strategies. A fusion protein STD was first expressed to serve as a backbone, consisting of streptavidin, a cell-penetrating peptide called Trans-Activator of Transcription (TAT) and a doublestranded RNA binding domain. A biotinylated Prostate Specific Membrane Antigen (PSMA) specific aptamer A10 and SURVIVIN-siRNA were then linked to STD protein to form the therapeutic complex. This complex could specifically targeted PSMA C tumor cells. Compared to lipofectamine and A10-siRNA chimera, it demonstrated higher efficiency in delivering siRNA into target cells by 19.2% and 59.9%, and increased apoptosis by 16.8% and 26.1% respectively. Upon systemic administration, this complex also showed significant efficacy in suppressing tumor growth in athymic mice (p <0.001). We conclude that this therapeutic complex could specifically and efficiently deliver SURVIVIN-siRNA to target cells and suppressed tumor growth in vivo, which indicates its potential to be used as a new strategy in prostate cancer therapy

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“…Aptamers have functions similar to those of antibodies, but they can be easily synthesized and conveniently preserved. Therefore, aptamers have an application potential as substitutes of antibodies in clinical diagnosis and disease treatment 1, 2, 3, 4, 5, 6, 7, 8. However, in fundamental research and clinical diagnosis, relevant applications are still dominated by monoclonal antibodies, and aptamer diagnostics is in the experimental stage, because the aptamer screening efficiency of the classical systematic evolution of ligands by exponential enrichment (SELEX) technology is relatively low 9, 10, and high‐quality aptamers are not easily available.…”

Section: Introductionmentioning

confidence: 99%

Construction and optimization of an efficient amplification method of a random ssDNA library by asymmetric emulsion PCR

Shao

Shi

Zhu

et al. 2016

Biotech and App Biochem

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Construction of a random ssDNA sublibrary is an important step of the aptamer screening process. The available construction methods include asymmetric PCR, biotin–streptavidin separation, and lambda exonuclease digestions, in which PCR amplification is a key step. The main drawback of PCR amplification is overamplification increasing nonspecific hybridization among different products and by‐products, which may cause the loss of potential high‐quality aptamers, inefficient screening, and even screening failure. Cycle number optimization in PCR amplification is the main way to avoid overamplification but does not fundamentally eliminate the nonspecific hybridization, and the decreased cycle number may lead to insufficient product amounts. Here, we developed a new method, “asymmetric emulsion PCR,” which could overcome the shortcomings of conventional PCR. In asymmetric emulsion PCR, different templates were separated by emulsion particles, allowing single‐molecule PCR, in which each template was separately amplified, and the nonspecific hybridization was avoided. Overamplification or formation of by‐products was not observed. The method is so simple that direct amplification of 40 or more cycles can provide a high‐quality ssDNA library. Therefore, the asymmetric emulsion PCR would improve the screening efficiency of systematic evolution of ligands by exponential enrichment.

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Aptamers as a replacement for antibodies in enzyme-linked immunosorbent assay

Cited by 475 publications

References 115 publications

Design and evaluation of split-ring resonators for aptamer-based biosensors

Design and evaluation of split-ring resonators for aptamer-based biosensors

A specific aptamer-cell penetrating peptides complex delivered siRNA efficiently and suppressed prostate tumor growthin vivo

Construction and optimization of an efficient amplification method of a random ssDNA library by asymmetric emulsion PCR

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