Applications of Titania Nanotubes in Bone Biology

Nair, Manitha B.; Elizabeth, Elmy

doi:10.1166/jnn.2015.9771

Cited by 24 publications

(22 citation statements)

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“…It has been reported that surface characteristics including physical topography and chemical components are identified as important factors affecting the subsequent osseointegration 34,35) . Implant surface with TiO2 nanotube arrays exhibited enhanced osseointegration and improvement of stability 36) . In particular, several previous studies have revealed higher bone-implant contact and gene expression levels of OCN, COL and ALP in the bone attached to 70-nm-diameter TiO 2 nanotubes [36][37][38] .…”

Section: Discussionmentioning

confidence: 99%

“…Implant surface with TiO2 nanotube arrays exhibited enhanced osseointegration and improvement of stability 36) . In particular, several previous studies have revealed higher bone-implant contact and gene expression levels of OCN, COL and ALP in the bone attached to 70-nm-diameter TiO 2 nanotubes [36][37][38] . It is also reported that HA could potentially improve osseointegration 39,40) , and Ca 2+ and PO4 3− ions provided necessary substances for osteoblast maturity and mineralization 35) .…”

Section: Discussionmentioning

confidence: 99%

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Improved osteoblast adhesion and osseointegration on TiO<sub>2</sub> nanotubes surface with hydroxyapatite coating

Song

et al. 2019

Dent. Mater. J.

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To improve initial osteoblast adhesion and subsequent osseointegration, TiO2 nanotubes layer was constructed on the titanium (Ti) surface by anodic oxidation (AO), with an additional hydroxyapatite (HA) coating to form the AO/HA surface. Tests on in vitro cellular activity displayed that the AO surface, especially the AO/HA surface, promoted initial adhesion, proliferation and differentiation of osteoblast cells. The modified AO and AO/HA surfaces further presented an up-regulated gene expression of osteogenic and adhesion markers collagen type 1 (COL), osteopontin (OPN), osteocalcin (OCN) and vinculin. In addition, in vivo experiments with a rat model demonstrated that the AO surface, particularly the AO/HA surface, achieved earlier osseointegration and a superior bone bonding ability compared with Ti. Our study shed light on a synergistic role played by nanotopography and HA in promoting osteoblast adhesion, proliferation, differentiation and osseointegration, thus suggesting a promising method for better modifying the implant surface.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Improved osteoblast adhesion and osseointegration on TiO<sub>2</sub> nanotubes surface with hydroxyapatite coating

Song

et al. 2019

Dent. Mater. J.

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“…Similarly, titanium (Ti)-based metallic materials have been widely optimized for bone repair due to their mechanical properties and resistance to corrosion following the transplantation [66][67][68]. It has been shown that titanium scaffolds are effectively colonized by osteoblasts responsible for bone formation and this process may be enhanced via additional modifications of the scaffold surface by its roughening, coating with HA or graphene oxide (GO), as well as its biofunctionalization with bioactive molecules such as heparin and bone morphogenetic protein 2 (BMP-2) [69][70][71][72].…”

Section: Synthetic Biomaterialsmentioning

confidence: 99%

Biomaterials and Stem Cells: Promising Tools in Tissue Engineering and Biomedical Applications

Sekuła¹,

Zuba‐Surma²

2018

Biomaterials in Regenerative Medicine

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Biomaterial sciences and tissue engineering approaches are currently fundamental strategies for the development of regenerative medicine. Stem cells (SCs) are a unique cell type capable of self-renewal and reconstructing damaged tissues. At the present time, adult SCs isolated from postnatal tissues are widely used in clinical applications. Their characteristics such as a multipotent differentiation capacity and immunomodulatory activity make them a promising tool to use in patients. Modern material technologies allow for the development of innovative biomaterials that closely correspond to requirements of the current biomedical application. Biomaterials, such as ceramics and metals, are already used as implants to replace or improve the functionality of the damaged tissue or organ. However, the continuous development of modern technology opens new insights of polymeric and smart material applications. Moreover, biomaterials may enhance the SCs biological activity and their implementation by establishing a specific microenvironment mimicking natural cell niche. Thus, the synergistic advancement in the fields of biomaterial and medical sciences constitutes a challenge for the development of effective therapies in humans including combined applications of novel biomaterials and SCs populations.

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“…Among the different strategies for surface modification, anodic oxidation has attracted much attention. Anodic oxidation (AO) is an easy, economical way to fabricate controllable nano-scale surface topography 7) . We have previously reported that the TiO 2 surface created by anodic oxidation induced bone-like apatite layer precipitation in simulated body fluid soaking test and increased the gene expression of bone-specific marker osteoprotegrin compared to the control Ti surface 8) .…”

Section: Introductionmentioning

confidence: 99%

Improved Cell Adhesion and Osseointegration on Anodic Oxidation Modified Titanium Implant Surface

Liu

You

et al. 2019

J. Hard Tissue Biology.

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Although titanium material is currently widely used in dental and orthopedic application, the bio-inertness of Ti impairs its further use. We previously reported the good bioactivity and biocompatibility of TiO 2 nanotube layer fabricated by anodic oxidation technique using in vitro tests. In the present work, we further clarify the cell adhesion and osseointegration properties of anodized surface through both in vitro and in vivo tests. After anodic oxidation (AO), the structure of nanotubes was confirmed by scanning electron microscopy. The surface roughness and hydrophilic properties of the AO and pristine Ti surfaces were evaluated by atomic force microscopy and contact angle measurement test, respectively. It was found that the AO surface displayed moderately higher surface roughness and obviously increased wettability, compared to the original Ti surface. In vitro cellular activity tests demonstrated that osteoblast cells cultured on the AO surface exhibited a much well-spread cytoskeleton organization with more stretched actin filaments, compared to those cultured on the Ti surface. The adherent cell number was also higher on the AO surface than the pure Ti substrate. In addition, we explore the molecular basis of mechanism by analyzing gene expression levels of adhesion and osteogenesis-related genes in MC3T3-E1 cells cultured on different surfaces using quantitative real-time PCR. Increased mRNA levels of vinculin, collagen type 1, osteopontin and osteocalcin were found on the AO surface, compared with the control group. Furthermore, in vivo animal experiment using a rat model revealed that anodized implant surface promotes osseointegration and demonstrated higher bone bonding strength compared to the pure Ti substrate. Our study revealed the superior cell adhesion property, increased adherent and osteogenesis-related gene expressions and enhanced osseointegration by anodized surface, thus implying its enlarged application in future.

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Applications of Titania Nanotubes in Bone Biology

Cited by 24 publications

References 0 publications

Improved osteoblast adhesion and osseointegration on TiO<sub>2</sub> nanotubes surface with hydroxyapatite coating

Improved osteoblast adhesion and osseointegration on TiO<sub>2</sub> nanotubes surface with hydroxyapatite coating

Biomaterials and Stem Cells: Promising Tools in Tissue Engineering and Biomedical Applications

Improved Cell Adhesion and Osseointegration on Anodic Oxidation Modified Titanium Implant Surface

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