Improved osteoblast adhesion and osseointegration on TiO₂ nanotubes surface with hydroxyapatite coating

Abstract

To improve initial osteoblast adhesion and subsequent osseointegration, TiO2 nanotubes layer was constructed on the titanium (Ti) surface by anodic oxidation (AO), with an additional hydroxyapatite (HA) coating to form the AO/HA surface. Tests on in vitro cellular activity displayed that the AO surface, especially the AO/HA surface, promoted initial adhesion, proliferation and differentiation of osteoblast cells. The modified AO and AO/HA surfaces further presented an up-regulated gene expression of osteogenic and adhesion markers collagen type 1 (COL), osteopontin (OPN), osteocalcin (OCN) and vinculin. In addition, in vivo experiments with a rat model demonstrated that the AO surface, particularly the AO/HA surface, achieved earlier osseointegration and a superior bone bonding ability compared with Ti. Our study shed light on a synergistic role played by nanotopography and HA in promoting osteoblast adhesion, proliferation, differentiation and osseointegration, thus suggesting a promising method for better modifying the implant surface.

Surface Immobilization of TiO2 Nanotubes with Bone Morphogenetic Protein-2 Synergistically Enhances Initial Preosteoblast Adhesion and Osseointegration

“…We previously studied the cellular response and bone binding ability of the combination of TiO 2 nanotube surface with chemical component hydroxyapatite (HA). It was revealed that the nanotopography and HA coating played a synergistic role in promoting osteoblast adhesion, proliferation, differentiation, and osseointegration [17].…”

Plasma Spray vs. Electrochemical Deposition: Toward a Better Osteogenic Effect of Hydroxyapatite Coatings on 3D-Printed Titanium Scaffolds

“…These findings suggest that PSHA scaffolds are more capable of accelerating the maturation of osteogenic mineralization. Furthermore, with regard to OPN and OCN expression (markers of osteogenic differentiation at a late stage) ( Li et al, 2019 ). The expression of OPN and OCN in the PSHA group was significantly higher than in the EDHA group.…”

Mechanical Properties of Different Nanopatterned TiO2 Substrates and Their Effect on Hydrothermally Synthesized Bioactive Hydroxyapatite Coatings

“…Moreover, the US substrate was successfully functionalized with hydroxyapatite coating (HApUS) and investigated for bioactivity. The combination of such specific nanotopography and bioactivity of HAp was previously reported to have a positive synergic effect on osseointegration in vivo [22]. Specifically, it was shown to upregulate the gene expressions of cell adhesion and osteogenic differentiation markers.…”