Application of supercritical fluid chromatography to the analysis of hydrophobic metabolites

Bamba, Takeshi

doi:10.1002/jssc.200700499

Cited by 30 publications

(15 citation statements)

References 28 publications

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“…However, the addition of a co‐solvent substantially increases the critical values of carbon dioxide . Column efficiencies obtained in SFC are comparable to those obtained in HPLC with identical particle size, but they are obtained in SFC at linear velocities that are three to five times greater than in HPLC . These advantages make SFC an advantageous separation technique that is widely used in the pharmaceutical industry for achiral and chiral separations on both analytic and preparative scales.…”

Section: Introductionmentioning

confidence: 94%

Fast separation of flavonoids by supercritical fluid chromatography using a column packed with a sub-2 μm particle stationary phase

et al. 2017

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The purpose of this study was to compare the effects of different chromatographic columns for the separation of seven flavonoids. Four different stationary phases are available, including bridged ethyl hybrid, BEH and the same hybrid phase modified with 2-ethylpyridine, CSH fluorophenyl, and HSS C SB. The analytes included calycosin, genistein, medicarpin, calycosin-7-O-β-d-glucoside, formononetin, formononetin-7-O-β-d-glucoside, and liquiritigenin. The CSH fluorophenyl column was determined to be the most suitable and provided the fastest separation within 17 min using gradient elution with carbon dioxide as the mobile phase and methanol as the co-solvent. Good peak shapes were obtained, and the values of the peak asymmetry were close to 1.0 for all of the flavonoids. The resolution was more than 1.41 for all of the separated peaks. Baseline separation on the optimal columns was achieved by changing the co-solvent type and adjusting the temperature and pressure. Quantitative performance was evaluated under optimized conditions, and method validation was accomplished. The validation parameters, such as linearity, sensitivity, precision, and accuracy, were satisfactory. Good repeatability of both peak area (relative standard deviation <1.02%) and retention time (relative standard deviation <0.88%) was observed. The optimized chromatographic methods were successfully used for the determination of seven flavonoids in Radix astragali. The sensitivity was sufficient for the analysis of real samples.

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Section: Introductionmentioning

confidence: 94%

Fast separation of flavonoids by supercritical fluid chromatography using a column packed with a sub-2 μm particle stationary phase

et al. 2017

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“…Another analytical method is supercritical fluid chromatography (SFC). It has been reported that the sensitivity of SFC was higher than that in LC, and several structural isomers that could not be separated by LC were successfully separated using SFC [31][32][33][34][35]. However, SFC also has several disadvantages.…”

Section: Introductionmentioning

confidence: 99%

Lipid profiling of cyanobacteriaSynechococcussp. PCC 7002 using two-dimensional liquid chromatography with quadrupole time-of-flight mass spectrometry

et al. 2016

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Glycerolipid is a main component of membranes in oxygenic photosynthetic organisms. Up to now, the majority of publication in this area has focused on the physiological functions of glycerolipids and lipoprotein complexes in photosynthesis, but the study on the separation and identification of glycerolipids in thylakoid membrane in cyanobacteria is relatively rare. Here we report a new method to separate and identify five photosynthetic glycerolipid classes, including monoglucosyl diacylglycerol, monogalactosyl diacylglycerol, digalactosyl diacylglycerol, sulfoquinovosyl diacylglycerol, and phosphatidylglycerol, in cyanobacteria Synechococcus sp. PCC 7002 by two-dimensional (normal- and reversed-phase) liquid chromatography online coupled to quadrupole time-of-flight mass spectrometry. Over twice as many lipid species were detected by our method compared to the previously reported methods. Ten new odd-chain fatty acid glycerolipids were discovered for the first time. Moreover, complete separation of isomers of monogalactosyl diacylglycerol and monoglucosyl diacylglycerol was achieved. According to the tandem mass spectrometry results, we found that the head group of monoglucosyl diacylglycerols was not as stable as that of monogalactosyl diacylglycerols, which might explain why the organism chose monogalactosyl diacylglycerols and digalactosyl diacylglycerols instead of monoglucosyl diacylglycerols as the main content of the photosynthetic membranes in the history of evolution. This work will benefit further research on the physiological function of glycerolipids.

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“…In this study, we developed a low‐temperature HPLC using liquid CO 2 as the mobile phase prior to ultra‐low‐temperature HPLC. It is well known that CO 2 is often used as the mobile phase of SFC . However, SFC separations are generally performed in a critical condition, i.e.…”

Section: Introductionmentioning

confidence: 99%

Chromatographic behavior of small organic compounds in low‐temperature high‐performance liquid chromatography using liquid carbon dioxide as the mobile phase

Motono

Nagai

Kitagawa

et al. 2015

J of Separation Science

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Low-temperature high-performance liquid chromatography, in which a loop injector, column, and detection cell were refrigerated at -35ºC, using liquid carbon dioxide as the mobile phase was developed. Small organic compounds (polyaromatic hydrocarbons, alkylbenzenes, and quinones) were separated by low-temperature high-performance liquid chromatography at temperatures from -35 to -5ºC. The combination of liquid carbon dioxide mobile phase with an octadecyl-silica (C18 ) column provided reversed phase mode separation, and a bare silica-gel column resulted in normal phase mode separation. In both the cases, nonlinear behavior at approximately -15ºC was found in the relationship between the temperature and the retention factors of the analytes (van't Hoff plots). In contrast to general trends in high-performance liquid chromatography, the decrease in temperature enhanced the separation efficiency of both the columns.

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Application of supercritical fluid chromatography to the analysis of hydrophobic metabolites

Cited by 30 publications

References 28 publications

Fast separation of flavonoids by supercritical fluid chromatography using a column packed with a sub-2 μm particle stationary phase

Fast separation of flavonoids by supercritical fluid chromatography using a column packed with a sub-2 μm particle stationary phase

Lipid profiling of cyanobacteriaSynechococcussp. PCC 7002 using two-dimensional liquid chromatography with quadrupole time-of-flight mass spectrometry

Chromatographic behavior of small organic compounds in low‐temperature high‐performance liquid chromatography using liquid carbon dioxide as the mobile phase

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