Application of a biofilm formed by a mixture of yeasts isolated in Vietnam to degrade aromatic hydrocarbon polluted wastewater collected from petroleum storage

Abstract

In this study, three good biofilm-forming yeast strains, including Candida viswanathii TH1, Candida tropicalis TH4 and Trichosporon asahii B1, were isolated from oil-contaminated water and sediment samples collected in coastal zones of Vietnam. These strains were registered in the GenBank database with the accession numbers JX129175, JX129176 and KC139404 for strain TH1, TH4 and B1, respectively. The biofilm formed by a mixture of these organisms degraded 90, 85, 82 and 67% of phenol, naphthalene, anthracene and pyrene, respectively, after a 7-day incubation period using an initial concentration of 600 ppm phenol and 200 ppm of each of the other compounds. In addition, this biofilm completely degraded these aromatic compounds, which were from wastewater collected from petroleum tanks in Do Xa, Hanoi after 14 days of incubation based on gas chromatography mass spectrometry analysis. To the best of our knowledge, reports on polycyclic aromatic hydrocarbon and phenol degradation by biofilm-forming yeasts are limited. The results obtained indicate that the biofilm formed by multiple yeast strains may considerably increase the degradation efficiency of aromatic hydrocarbon compounds, and may lead to a new approach for eliminating petroleum oil-contaminated water in Vietnam.

On and Under the Skin: Emerging Basidiomycetous Yeast Infections Caused by Trichosporon Species

“…Trichosporon species are basidiomycetous, yeast-like organisms capable of filamentous growth, i.e., dimorphic ( Fig 1 ), that are distributed throughout nature [ 1 ]. They are important from a biotechnological point of view as they are capable of decontaminating polluted environments by accumulating large amounts of oils [ 2 – 5 ]. A limited number of reports also show their presence in the human microbiome, such as Trichosporon asahii , which has been isolated from human fecal samples, the skin of healthy individuals, and patients with atopic dermatitis [ 1 , 6 – 10 ].…”

Design of Bio-Absorbent Systems for the Removal of Hydrocarbons from Industrial Wastewater: Pilot-Plant Scale

“…At the genus level, Rhodotorula ( Sporidiobolales ), Didymella ( Pleosporales ), and Candida ( Sacharomycetalles ) were the most abundant genera in all the systems. These are unicellular fungi belonging to Basidiomycota and Ascomycota that have been previously shown to form biofilms, which could explain the abundance of these fungi in the Pad samples [ 37 , 38 ]. Despite most studies on fungal communities focusing on hydrocarbon-polluted soils, similar communities have also been found in wastewater.…”

Recent advances in fungal xenobiotic metabolism: enzymes and applications

“…Confocal laser scanning microscopy showed co-localisation of fluoranthrene within the extracellular polymeric substance enhancing biotransformation. A mixed biofilm of the yeasts Candida viswanathii TH1, Candida tropicalis TH4 and Trichosporon asahii B1, that were isolated from oil-contaminated environments, degraded a mixture of PAHs (naphthalene, anthracene and pyrene, 200 ppm each) and phenol (600 ppm) over 7 days (Cong et al 2014 ).…”