Antiviral Action of Hydromethanolic Extract of Geopropolis fromScaptotrigona posticaagainst Antiherpes Simplex Virus (HSV-1)

Abstract

The studies on chemical composition and biological activity of propolis had focused mainly on species Apis mellifera L. (Hymenoptera: Apidae). There are few studies about the uncommon propolis collected by stingless bees of the Meliponini tribe known as geopropolis. The geopropolis from Scaptotrigona postica was collected in the region of Barra do Corda, Maranhão state, Brazil. The chemical analysis of hydromethanolic extract of this geopropolis (HMG) was carried out through HPLC-DAD-ESI-MS/MS and the main constituents found were pyrrolizidine alkaloids and C-glycosyl flavones. The presence of alkaloids in extracts of propolis is detected for the first time in this sample. The antiviral activity of HMG was evaluated through viral DNA quantification experiments and electron microscopy experiments. Quantification of viral DNA from herpes virus showed reduction of about 98% in all conditions and concentration tested of the HMG extract. The results obtained were corroborated by transmission electron microscopy, in which the images did not show particle or viral replication complex. The antiviral activity of C-glycosyl flavones was reported for a variety of viruses, being observed at different points in the viral replication. This work is the first report about the antiviral activity of geopropolis from Scaptotrigona postica, in vitro, against antiherpes simplex virus (HSV).

Insects: an underrepresented resource for the discovery of biologically active natural products

“…The chemical composition was found to consist mostly of pyrrolizidine alkaloids and C -glycosyl flavones. Treatment with the extract reduced the presence of HSV-1 by 98% which was attributed to inhibition of viral replication and virus entry into cells 76 . Propolis from the Brazilian stingless bee, Tetragonisca fiebrigi , was evaluated for antimicrobial, antioxidant and anti-inflammatory activity 77 .…”

Atividade antiviral e virucida de extratos hidroalcoólicos de própolis marrom, verde e de abelhas Jataí (Tetragonisca angustula) frente ao herpersvírus bovino tipo 1 (BoHV-1) e ao vírus da diarreia viral bovina (BVDV)

“…Resultado semelhante foi encontrado no presente estudo ao utilizar-se o extrato de jataí que apresentou perfil químico semelhante ao dos autores, e atividade antiviral significativa frente ao BVDV quando no tratamento antes da inoculação viral. Coelho et al (2015) avaliaram a ação antiviral de um extrato hidroetanólico de Scaptotrigona postica oriundo da região de Barra de Corda, estado do Maranhão, Brasil frente ao Herpes Simplex Vírus (HSV-1). A análise química da própolis foi realizada por meio de HPLC-DAD-ESI /MS, e a mensuração da atividade antiviral da própolis foi realizada através da quantificação do DNA e microscopia eletrônica.…”

“…Estudos sobre a atividade antiviral da própolis e seus derivados já foram descritos frente a vírus de importância em medicina e medicina veterinária como o herpes vírus humano tipo 1 (HSV1) (Amoros et al 1992, Coelho et al 2015, Yildirim et al 2016) e dois (Vynograd et al 2000, Yildirim et al 2016, vírus da imunodeficiência humana (HIV) (Gekker et al 2005), vírus da influenza aviária (Kujumgiev 1999), Herpes Vírus Bovino (BoHV-5) (Fischer et al 2007) e o Parvovírus Suíno (PPV) (Ma et al 2015) e demonstraram resultados promissores da própolis frente a vírus patogênicos para humanos e animais.…”

Chemical Profile and Antioxidant, Anti-Inflammatory, Antimutagenic and Antimicrobial Activities of Geopropolis from the Stingless Bee Melipona orbignyi

“…Regarding its chemical composition, the presence of phenylpropanoids, flavonoids [10], phenolic acids, hydrolysable tannins [7], triterpenes, saponins [14] and alkaloids [13] have been described, compounds that may be related to the biological properties of this bee product. Despite the growing interest in the pharmacological properties of the geopropolis produced by the Melipona , the data in the literature remain very scarce compared to those on the propolis of the Apis mellifera species.…”