Antioxidant status and lipid peroxidation after short-term maximal exercise in trained and untrained humans

Abstract

The purpose of this study was to measure resting muscle and blood antioxidant status in untrained (n = 8) and jump-trained (n = 8) humans and to evaluate free radical-mediated muscle damage after a strenuous jump test consisting of six bouts of 30-s continuous jumping separated by 2 min of rest. Resting muscle antioxidant activities [superoxide dismutase (SOD), glutathione peroxidase (GPX), glutathione reductase (GR), and manganese SOD] were significantly higher in jump-trained compared with untrained subjects. Blood antioxidant enzyme activities and muscle catalase, however, were not different between the two groups. Creatine kinase activities increased significantly (P < 0.0001) after the jump test in untrained individuals, but remained unchanged in the jump trained. Plasma and muscle malonaldehyde (MDA) after the jump test were not significantly different from rest. These data suggest that jump training is associated with elevated activities of SOD and the coupled enzymes GPX and GR in muscle tissue, but other antioxidants remain unchanged. High-intensity jump exercise induces muscle enzyme leakage in untrained humans, but muscle lipid peroxidation, measured as changes in MDA, was not different in the two groups despite the varied muscle antioxidant enzyme levels.

Oxidative profiles of endurance horses racing different distances

“…The chronic training promotes definitive increases in these defences. Additionally, Brites et al (1999) and Ortenblad et al (1997) found that trained human athletes had higher baseline concentrations of this enzyme than did untrained individuals, which is in accordance with the results of the present study maybe because, according to new FEI rules for the horses competing in 80km races, they must be graduated first, so they necessarily trained for at least two years.…”

Inactivity and Inflammation in the Critically Ill Patient

“…These antioxidants are produced by skeletal muscles [53]. Repetitive training can increase antioxidant synthesis and activity in cells and healthy people [58,59].…”

Exercise and Oxidative Stress

“…Many studies have compared the antioxidant status of trained and untrained subjects at rest (Ørtenblad et al, 1997;Balakrishnan & Anuradh 1998;Brites et al, 1999;Evelson et al, 2002;Cazzola et al, 2003;Gougoura et al, 2007;Yamaner, 2010), but, to our knowledge, except the study of Ørtenblad and coworkers (1997) that analyzed blood antioxidant status in untrained and jump-trained humans following six bouts of 30-s continuous jumping, no study has compared the athletes' and non-athletes' pro/antioxidant responses to acute exercise of maximal intensity. The hypothesis of the first part of that study was that athletes would have not only higher activity of antioxidants in rest, but that the extent of redox disturbance induced by a maximal progressive exercise test would be lower in athletes compared with non-athletes.…”