Antioxidant, antimicrobial and wound healing properties ofStruthanthus vulgaris

Abstract

Context: Struthanthus vulgaris (Vell.) Mart. (Loranthaceae) has been widely used in traditional medicine in Brazil to bathe wounds. Objective: The objective of this study is to investigate the in vitro wound healing effects, together with the antioxidant and antimicrobial activities of S. vulgaris leaf and branch extracts. Material and methods: Ethanol leaf and branch extracts of S. vulgaris were investigated at 1-100 mg/ml concentrations in the scratch assay after 14 h. Antioxidant activity was investigated using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical-scavenging assay, and the antibacterial activity was tested at concentrations up to 1000 mg/ml against Gram-positive and Gramnegative bacteria by the microdilution test after 24 h. The total phenolic and flavonoid contents were determined by colorimetric methods. Results: Struthanthus vulgaris leaf and branch extracts at 100 mg/ml concentration stimulated migration and proliferation of fibroblasts and enhanced cell numbers by 56.2% and 18.6%, respectively. Antioxidant activity exhibited IC 50 values of 24.3 and 18.9 mg/ml for the leaf and branch extracts, respectively. The ethanol leaf extract showed antimicrobial activity against the Gram-positive Staphylococcus mutans and Staphylococcus aureus bacteria, exhibiting minimum inhibitory concentration values of 125 and 500 mg/ml, respectively. An appreciable total phenolic content in the leaves (813.6 ± 2.7 mg/g) and branches (462.8 ± 9.6 mg/g), and relatively low concentration of flavonoids in the leaves (13.3 ± 4.3 mg/g) and branches (1.9 ± 0.2 mg/g), was detected. Discussion and conclusion: The antioxidant and antibacterial activities, together with the strong ability to stimulate proliferation and migration of fibroblasts, provide some support for the traditional use of S. vulgaris.

Struthanthus vulgaris ointment prevents an over expression of inflammatory response and accelerates the cutaneous wound healing

“…Therefore, the well-formed collagen fibers observed in S. vulgaris treated group by histomorphometric analysis and biochemical determination of hydroxyproline could support the S. vulgaris effectiveness in fibroblastic proliferation and synthesis the extracellular matrix during wound healing. Corroborating to our results, Vittorazzi et al, (2015) demonstrated the in vitro beneficial effects of S. vulgaris in wound healing processes by its ability to stimulate the migration and proliferation of fibroblast in the wound scratch assay. Furthermore, stimulation of epithelial cell proliferations are vital for wound healing to take place.…”

“…The biological observed effects were attributed to the presence of flavonoids, condensed tannins (proanthocyanidins) and saponins in the leaf extract (Vieira et al, 2005). Recently, Vittorazzi et al (2015) showed the antioxidant and antibacterial activities of S. vulgaris together with the in vitro stimulatory effects on proliferation and migration of fibroblasts.…”

“…S. vulgaris has been largely used in traditional folk medicine as an anti-inflammatory agent and to treat various skin disorders, including wounds (Salatino et al, 1993;Vieira et al, 2005;Conceição et al, 2010;Vittorazzi et al, 2015). Although its popularity, there are only few scientific studies that refer to S. vulgaris.…”

Investigating of Phytochemicals, Antioxidant, Antimicrobial and Proliferative Properties of Different Extracts of Thymus spathulifolius Hausskn. and Velen. Endemic Medicinal Plant from Sivas, Turkey

“…The In vitro proliferative activity was evaluated in the colorimetric MTT assay [28]. Exponential growing L929 Mouse fibroblast cells were plated in 96-well micropates at a density of 5 × 10 3 cells per well in 100 μL of culture medium and were allowed to adhere for 16 h, cultured in a humidified atmosphere at 37 °C in 5% CO2 before treatment.…”

Healing Effect of Hawthorn (Crataegus pontica C. Koch) Leaf Extract in Dermal Toxicity Induced by T-2 Toxin in Rabbit

“…These components prevent blood and lymph accumulation by regulating micro vessels around the wound lesion area and thus reduce local swelling. They also improve blood circulation and enhance continuous perfusion to facilitate the healing of wound lesions (22,23). Since the most important class of active ingredients present in Crataegus sp.…”