Investigating of Phytochemicals, Antioxidant, Antimicrobial and Proliferative Properties of Different Extracts of Thymus spathulifolius Hausskn. and Velen. Endemic Medicinal Plant from Sivas, Turkey

Abstract

Thymus species has been used for antioxidant, antiseptic, antitussive, carminative, anti-inflammatory and antimicrobial activities as well as tonic and herbal teas. The present study was conducted to evaluate antioxidant, antimicrobial and proliferative properties of ethanol crude extract and fractions of Thymus spathulifolius (Hausskn. and Velen.) herbes. The antioxidant properties of ethanol extracts and fractions of Thymus spathulifolius were investigated using 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity, reducing power, ferrous chelating activity, total flavonoid and total phenolic content analysis. Antimicrobial activity of the plant extracts were tested using the microdilution method, while proliferative activity were evaluated by MTT assay. Results showed that IC50 of T. spathulifolius extracts that scavenged 50% of the DPPH radical was found to be ranged from 62.39 to 1000 µg/mL. Hexane extract possessed moderate antimicrobial activity towards grampositive bacteria of S. aureus and fungi of C. albicans. S. aureus was the most sensitive bacteria than other tested microorganisms. The hexane and water extracts exhibited a good proliferative activity with ED50 of 3.28 µg/mL and 2.77 µg/mL, respectively. The antioxidant and antimicrobial activities, together with the ability of proliferation, provide some support for the T.spathulifolius's traditional use.

Antioxidant and Antibacterial Effects of Some Medicinal Plants of Iran

“…The beneficial medicinal effect of a plant is due to the secondary metabolites in the plant [50][51][52][53]. There are alkaloids, flavonoids and phenolic compounds in the methanol extract of E. khuzistanicum flower according to GC/MS analysis that similar results have been described by Tiwari et al (2011) [50].…”

In Vitro Evaluation of Antioxidant, Anti-Inflammatory, Antimicrobial and Wound Healing Potential of Thymus Sipyleus Boiss. Subsp. Rosulans (Borbas) Jalas

“…In addition, H 2 O 2 -induced oxidative stress has been reported as an alternative to study the antioxidant activities of plant extracts in cells [31]. Previous studies on different Thymus species have shown that this plant imparts antioxidant effects that play a crucial role in healing process by protecting cells from oxidative damage caused by free radicals [32,33,34,35]. In the present study, the protection degrees of TS extracts against oxidative stress were differed in H 2 O 2 -treated cells, both in the co-treatment and pre-treatment protocols, and this may explain the possible mechanism of antioxidant effect.…”

BIOLOGICAL ACTIVITY EVALUATION OF THREE Hypericum L. SPECIES

“…The in vitro cytotoxic activities of the extracts were evaluated by the MTT colorimetric assay according to Mosmann (1983) and Eruygur et al (2017). Prostate cancer (PC3) cell lines with exponential growth were planted into 96-well microplates at a density of 5×10 3 cells per well in 100 μL of culture medium and allowed to adhere for 16 hours before being cultured at 37°C in a 5% CO2 humidified environment.…”