Antioxidant and antibacterial activity of extracts, fractions and isolated substances from the flowers of Acacia podalyriifolia A. Cunn. ex G. Don

Abstract

The extracts and fractions from the flowers of A. podalyriifolia were analyzed previously for antibacterial activity using diffusion in disk, Antioxidant properties were evaluated by determining radical scavenging power (DPPH test) and total phenol content was measured (Folin method). The present study describes the in vitro antibacterial (determining minimum inhibitory concentration) and antioxidant activities (by thiobarbituric acid reactive species -TBARS method) for the ethanol extract, dichloromethane and ethyl acetate fractions and two flavanones (naringenin and 5-β-D-glycosyl-naringenin) isolated from the flowers of Acacia podalyriifolia A. Cunn. ex G. Don. The flavanones naringenin and 5-β-D-glycosylnaringenin had not previously been obtained from this species. The most effective antibacterial activity was observed in the ethyl acetate fraction (MIC=0.25 mg mL -1 against Staphylococcus aureus ATCC 6538, MIC = 0.125 mg mL -1 against Staphylococcus epidermidis ATCC 12229, MIC=0.5 mg mL -1 against Streptococcus pyogenes ATCC 19615, Klebsiella pneumoniae ATCC 13883 and Proteus mirabilis ATCC 43071). The evaluated samples showed antioxidant activity on the TBARS test, especially for ethanol extract (1000 ppm), which was the most active (29.43% ± 0.65) followed by ethyl acetate fraction (1000 ppm, 24.84% ± 1,28), both demonstrating higher activity than that presented by ascorbic acid (1000 ppm, 21.73% ± 1.77), although lower than the BHT (1000 ppm 35.15% ± 3.42), both reference compounds. Naringenin and 5-β-D-glycosyl-naringenin demonstrated antioxidant action, but only naringenin inhibited the growth of gram-positive and gram-negative bacteria.Uniterms: Acacia podalyriifolia/qualitative analysis. Acacia podalyriifolia/phytochemistry. Acacia podalyriifolia/antibacterial activity. Acacia podalyriifolia/antioxidant properties. Flavanones. 5-b-Dglycosyl-naringenin. Naringenin.Os extratos e frações de Acacia podalyriifolia foram analisados previamente para a atividade antibacteriana através da difusão em disco e as propriedades antioxidantes foram verificadas pela determinação da capacidade removedora do radical livre DPPH e pela mensuração do conteúdo de fenólicos totais (Método de Folin). O presente estudo descreve as atividades antibacteriana (determinação da concentração inibitória mínima) e antioxidante (espécies reativas do ácido tiobarbitúrico -teste TBARS) para o extrato etanólico e as frações diclorometano e acetato de etila e para duas flavanonas (naringenina e 5-b-D-glicosilnaringenina) isoladas das flores de Acacia podalyriifolia A. Cunn. ex G. Don. As flavanonas naringenina e 5-b-D-glicosil-naringenina ainda não haviam sido obtidas desta espécie. A atividade antibacteriana mais efetiva foi observada com a fração acetato de etila (CIM=0,25 mg/mL contra Staphylococcus aureus ATCC 6538; CIM=0,125 mg/mL, contra Staphylococcus epidermidis ATCC 12229; CIM=0,5 mg/mL contra Streptococcus pyogenes ATCC 19615, Klebsiella pneumoniae ATCC 13883 e Proteus mirabilis ATCC 43071). As amostras avaliadas demonstraram a...

Bioactive Compounds from the Roots of Asiasarum heterotropoides

“…The 21 known compounds 2 – 22 were identified as (+)-7'-methoxylariciresinol ( 2 ) [18],(–)-tanegool-7'-methyl ether ( 3 ) [18], aristolactam I ( 4 ) [19], 7-methoxyaristololactam IV ( 5 ) [20,21], aristolochic acid IVa ( 6 ) [22], (7 α ,7' β ,8 α ,8' α )-3,4-methylenedioxy-3',4'-dihydroxy-7,9':7',9-diepoxy-lignane ( 7 ) [23], (–)-asarinin ( 8 ) [24], (+)-xanthoxylol ( 9 ) [25], (–)-epipinoresinol ( 10 ) [25],(–)-sesamin ( 11 ) [26], (–)-piperitol ( 12 ) [27,28], (1 R ,2 S ,5 R ,6 R )-5'- O -methylpluviatilol ( 13 ) [29], (2 S )-naringenin ( 14 ) [30], (2 S )-naringenin-5- O - β - d -glucopyranoside ( 15 ) [31], (2 S )-naringenin-7- O - β - d -glucopyranoside ( 16 ) [32], (+)-asarinol D ( 17 ) [33], (±)-asarinol A ( 18 ) [34], isovanillic acid ( 19 ) [35], 4-hydroxybenzoic acid ( 20 ) [36], trans - p -feruloyl- β - d -glucopyranoside ( 21 ) [37], and ( R )-5-(2,3-dihydroxypropyl)-1,3-benzodioxole ( 22 ) [38]. Thirteen of these compounds ( 2 , 3 , 7 , 10 , 12 – 17 , 19 , 21 , and 22 ) were isolated from A. heterotropoides for the first time in this study.…”

Application of Green Technology for the Acquisition of Extracts of Araçá (Psidium grandifolium Mart. ex DC.) Using Supercritical CO2 and Pressurized Ethanol: Characterization and Analysis of Activity

“…The antimicrobial effects of the extracts were tested according to the methodology described by Andrade et al. () with some modifications. Pure bacterial strains were subcultured in 10 mL of Mueller–Hilton infusion and incubated at 37 °C for 24 hr.…”

Analgesic, Anti-Inflammatory, and Antioxidant Activities of Byrsonima duckeana W. R. Anderson (Malpighiaceae)

“…The final reaction mixture was then extracted with n -butanol and centrifuged for 3 minutes. The absorbance values of the supernatants were measured at 532 nm to detect the formation of malondialdehyde (MDA) indicating the decomposition of polyunsaturated fatty acid compounds [ 16 ]. The analysis was performed in quintuplicate.…”