Bioactive Compounds from the Roots of Asiasarum heterotropoides

Lee, Jun; Lee, You Jin; Oh, Se-Mi; Yi, Jin-Mu; Kim, No Soo; Bang, Ok‐Sun

doi:10.3390/molecules19010122

Cited by 15 publications

(13 citation statements)

References 55 publications

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“…However, their effects on various types of skin cancer cells have not been fully studied. Although they have been reported to exhibit anticancer effects on other types of cancers, including colon cancer [16], lung cancer [17, 18], and cervical cancer, specific mechanisms of action have not been uncovered.…”

Section: Discussionmentioning

confidence: 99%

Ethanol extract of asiasari radix preferentially induces apoptosis in G361 human melanoma cells by differential regulation of p53

Park

Choi

Song

et al. 2019

BMC Complement Altern Med

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Background: In Korea and China, asiasari radix (AR) is widely used as a traditional anti-inflammatory and analgesic agent. After its skin-regenerating and hair loss-preventing activities were identified, several types of AR extracts were used for aesthetic purposes. Nevertheless, the effect of ARE on various types of skin cancers was not fully studied yet. Methods: In this study, we tested the effect of an ethanolic AR extract (ARE) on G361 human melanoma and HaCaT human keratinocyte cell lines. After ARE exposure, cell growth and the expression patterns of proteins and genes were monitored. Results: The ARE-mediated cell growth inhibition was greater in G361 cells than in HaCaT cells due to differences in its cell growth regulation effects. Interestingly, ARE treatment induced caspase-3-mediated apoptosis in G361 cells, but not in HaCaT cells. Furthermore, ARE reduced the expression of p53 and p21 proteins in G361 cells, whereas it induced their expression in HaCaT cells. ARE induced cell death in G361 cells through the reactive oxygen species (ROS)-dependent regulation of p53 and p21 in G361 cells. Microarray analysis showed that ARE regulates Mouse double minute 2 homolog (MDM2) and CASP8 and FADD-like apoptosis regulator (CFLAR) gene expression in G361 and HaCaT cells differently. Conclusion: The treatment of ARE preferentially induces apoptosis in melanoma cells by the ROS-dependent differential regulation of p53 level. Therefore, ARE can be used as a new medicinal option for melanoma.

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Section: Discussionmentioning

confidence: 99%

Ethanol extract of asiasari radix preferentially induces apoptosis in G361 human melanoma cells by differential regulation of p53

Park

Choi

Song

et al. 2019

BMC Complement Altern Med

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“…Previous phytochemical investigations of A. heterotropoides var. mandshuricum resulted in the identification of chemical constituents with diverse structures including volatile oil [2], lignans [3,4,5,6], amides [7], terpenes [8], flavones [6,9,10,11,12], and benzene derivatives [6,11,12,13,14]. Additionally, the volatile oil of AR was considered the main effective component contributing to analgesia and anti-inflammation [15,16].…”

Section: Introductionmentioning

confidence: 99%

“…According to the previous results, it could be concluded that non-volatile constituents existed in AR also had the anti-inflammatory and analgesic activities. However, although AR worked to control pain and inflammation in traditional Chinese medicine prescriptions, there was not enough research to explain its non-volatile active components of analgesia and anti-inflammation, in spite of a few previous papers reporting its potential anti-tussive, anti-tumor, and anti-inflammatory effects [5,6,22,23], along with anti-allergic and anti-inflammatory components of Asiasarum sieboldi F. Maekawa belonging to the same genus [24,25,26]. …”

Section: Introductionmentioning

confidence: 99%

Chemical Constituents from the Roots and Rhizomes of Asarum heterotropoides var. mandshuricum and the In Vitro Anti-Inflammatory Activity

et al. 2017

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Anti-inflammatory compounds were investigated from the ethanol extract of the roots and rhizomes of Asarum heterotropoides var. mandshuricum, a traditional Chinese medicine called Xixin and used for pain and inflammatory. Nine new compounds were isolated, including six new lignans, neoasarinin A–C (1–3), neoasarininoside A and B (4 and 5), and asarinin B (7), and one new monoterpene, asarincin A (8), two new amides, asaramid II and III (10 and 11), and one new natural monoterpene, asaricin B (9), along with 37 known compounds (6, 12–47). Their structures and absolute configurations were elucidated on the basis of spectroscopic methods and chemical analyses. This is the first report of the absolute configuration of asarinin A (6). The 8-O-4′ neolignans (1–5) were reported in the genus Asarum for the first time. The 15 compounds 17, 19, 22–25, 28, 31, 36, 40, 42, 43, 45–47 were isolated from the genus Asarum, and compounds 16, 32, 33, 37 and 39 were isolated from A. heterotropoides var. mandshuricum for the first time. Thirty-seven of the isolates were evaluated for anti-inflammatory activity against the release of β-glucuronidase in polymorphonuclear leukocytes (PMNs) induced by the platelet-activating factor (PAF), and compounds 1, 4, 7, 8, 14, 17–19, 22, 24, 25, 29, 30, 32, 33, 40–43, 45, and 46 showed potent anti-inflammatory activities in vitro, with 27.9%–72.6% inhibitions at 10−5 mol/L. The results of anti-inflammatory assay suggested that lignans obtained from the CHCl3 extract might be the main active components of Xixin.

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“…Thus, the structures of compounds 2 and 3 were determined as shown in Figure 1, and they were given the trivial names phengustifol A and B, respectively. There are many reports about the cytotoxicity of triterpenoid saponins and lignan glucosides against various cell lines [15][16][17][18], thus, the three new compounds obtained in the current study were tested for cytotoxic activity against the A375 human melanoma cell line using the CCK8 method [19]. The results revealed that compounds 1 and 2 exhibited moderate cytotoxic activities, with IC 50 values at 12.1 and 15.6 µM, respectively, while compound 3 showed weak cytotoxicity with an IC 50 value of 62.8 µM.…”

Section: Resultsmentioning

confidence: 99%

Triterpenoid Saponin and Lignan Glycosides from the Traditional Medicine Elaeagnus angustifolia Flowers and Their Cytotoxic Activities

et al. 2020

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A new triterpenoid saponin, named terpengustifol A (1), and two new lignan glucosides, phengustifols A and B (2 and 3), were isolated from the flowers of Elaeagnus angustifolia. Their structures were determined by the extensive analysis of the spectroscopic data (including NMR and HRMS) and ECD calculations. Compound 1 possesses an unusual monoterpene (Z)-6-hydroxy-2,6-dimethylocta-2,7-dienoyl unit at C-21. Compounds 2 and 3 are a pair of diastereoisomers, while their aglycones are a pair of enantiomers. Compounds 1 and 2 exhibited moderate cytotoxic activities against A375 cell lines with IC50 values at 12.1 and 15.6 μM, respectively. This is firstly reported the triterpenoid saponin and lignans isolated from the Elaeagnus angustifolia flowers.

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Bioactive Compounds from the Roots of Asiasarum heterotropoides

Cited by 15 publications

References 55 publications

Ethanol extract of asiasari radix preferentially induces apoptosis in G361 human melanoma cells by differential regulation of p53

Ethanol extract of asiasari radix preferentially induces apoptosis in G361 human melanoma cells by differential regulation of p53

Chemical Constituents from the Roots and Rhizomes of Asarum heterotropoides var. mandshuricum and the In Vitro Anti-Inflammatory Activity

Triterpenoid Saponin and Lignan Glycosides from the Traditional Medicine Elaeagnus angustifolia Flowers and Their Cytotoxic Activities

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