Antioxidant activity of methanol extract of Diplazium esculentum (Retz.) Sw. leaves collected from Aceh

Abstract

Cell damage mediated by free radicals is one of the main causes of many dangerous diseases such as cancer, autoimmune disorders, rheumatism, cataracts, aging, cardiovascular disease, diabetes, arthritis, Parkinson, Alzheimer and neurodegenerative diseases. The prevention of dangerous diseases caused by free radicals can be done by developing raw materials for natural antioxidant drugs that can reduce free radicals by giving one of their electrons to produce neutral molecules that are not harmful to the human body, such as fern (Diplazium esculentum (Retz.) Sw.). D. esculentum collected from Aceh was extracted in methanol. The antioxidant activity of this extract was determined by 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity assay. The result showed very good activity of the DPPH antiradical efficiency of methanol extract of D. esculentum with IC50 values of 123.958 ppm. Based on phytochemical screening, D. esculentum contained polyphenol compounds that have good activity as antioxidant. This result indicated that D. esculentum has potential as antioxidant and can be applied in the development of new medicines.

Anticancer, antioxidant, and antibacterial activities of the methanolic extract from Sphagneticola trilobata (L.) J. F pruski leaves

“…The antioxidant activity was quantitatively analysis in vitro carried out using 2,2-diphenyl-1-picrylhydrazyl (DPPH) method (in triplicate) as previously described. [ 6 15 ] The extract solution with different concentrations (25–200 μg/mL) was prepared by dissolving the extract using mL methanol p.a. As much as 4 mL dissolved extract was then mixed with 1 mL (0.4 mM), followed by 30 min incubation in a dark condition at 37°C and measured using ultraviolet-visible spectrophotometer (Infinite M200, Tecan, Switzerland) at'.…”

Antioxidant and cytotoxic activities of the ethyl acetate extract of Sphagneticola trilobata (L.) J.F. Pruski on MCF-7 breast cancer cell

“…The study used ascorbic acid as a standard antioxidant and antioxidant capacity was expressed as IC 50 . [ 3 ]…”

“…If the body exposed to free radicals for a long time, it will increase the progression of degenerative diseases including aging, cancer, arteriosclerosis, diabetes, Alzheimer's and Parkinson's diseases, and immune deficiency diseases. [ 2 3 ]…”

“…[ 6 ] Thus, discovery and development of natural antioxidants should continue to be carried out due to its very valuable in the pharmaceutical field. [ 3 ] One source of natural antioxidant from herbs is Sphagneticola trilobata (L.) J. F Pruski. [ 4 ]…”

Identification of Phytochemicals, Antioxidant Activity Using DPPH Method (1.1=Diphenyl-2-Picrylhydrazyl), Toxicity Using Brine Shrimp Lethality Test of Diplazium esculentum Tea

“…Flavonoids as antioxidants can prevent the emergence of diseases caused by free radicals (Alfonita, 2018). Polyphenol compounds from plants have many benefits, including antioxidants, and can be applied to develop new drugs (Halimatussakdiah et al, 2020). Natural phenolic compounds that are antioxidants can be classified into lipophilic and hydrophilic groups (including phenolic compounds).…”