Antioxidant and cytotoxic activities of the ethyl acetate extract of Sphagneticola trilobata (L.) J.F. Pruski on MCF-7 breast cancer cell

Mardina, Vivi; Harmawan, Tisna; Halimatussakdiah, Halimatussakdiah; Tanjung, Masitta

doi:10.4103/japtr.japtr_31_20

Cited by 10 publications

(9 citation statements)

References 18 publications

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“…[12] reported that secondary metabolites of flavonoids and steroids have the potential to prevent and treat cancer with high cytotoxic activity using the Brine Shrimp Lethality Test (BSLT) method. Sphagneticola trilobata contains metabolite compounds such as flavonoids and steroids that have the potential to prevent and treat cancer [6]. The role of plant compounds as prevention and repair by preventing karsinogens from reaching the target location (initiation), inhibiting malignant transformation of cells during the promotion or progression phase so that the growth of proliferating cells is not controlled so that cancer cells will be inhibited from metastasizing.…”

Section: Resultsmentioning

confidence: 99%

Effect of Sphagneticola Trilobata Extract on Histological Wistar Rat Kidney Induced by DMBA

Surbakti

Mardina

Fajar

2021

Int. J. Eng. Scie. and Inform. Technology.

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One factor can trigger the growth and development of cancer cells is free radicals that are from carcinogenic compounds such as dimethylbenz (α) anthracene (DMBA). The use of plant extracts as a preventive or curative in cases of tumors/cancer has been reported, however there is no reports about the in vivo study for Sphagneticola trilobata plant. S. trilobata is an herbal plant that has pharmacological activities, potential to be developed as anticancer agent. This study aims to examine the anticancer effect of the methanol extract of S. trilobata leaves using histology observation on Wistar rat (Rattus novergicus) kidney which was induced by 7,12 dimethylbenz [α] anthracene (DMBA). The study used a completely randomized design with female rats (15 rats) grouped into 5 treatment groups, namely (i) the normal treatment group (KN), (ii) the DMBA-only treatment group (negative control, K(-)), (iii) the first dose (200 mg / kg BW) treatment group (KI), (iv) the second dose (300 mg / kg BW) treatment group (KII), and (v) the third dose (400 mg / kg BW) treatment group (KIII). DMBA was given orally at a concentration of 18 mg / kg BW for 4 times then continued for the extract. The results showed that cell damages (degeneration, necrosis and inflammation) were found mostly in negative controls. The dosage of 200 mg / kg BW of S. trilobata extract was the optimum dose in this study which was able to inhibit histological damage of kidney organs exposed to carcinogens DMBA by decreasing the level of degeneration, necrosis and inflammation.

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Section: Resultsmentioning

confidence: 99%

Effect of Sphagneticola Trilobata Extract on Histological Wistar Rat Kidney Induced by DMBA

Surbakti

Mardina

Fajar

2021

Int. J. Eng. Scie. and Inform. Technology.

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“…The methanolic extract was then phytochemically screened for the presence of flavonoids, alkaloids, saponins, steroids, tannins, and phenols employing the procedures used in our previous report. [ 6 ]…”

Section: Methodsmentioning

confidence: 99%

“…The antioxidant activity was quantitatively analysis in vitro carried out using 2,2-diphenyl-1-picrylhydrazyl (DPPH) method (in triplicate) as previously described. [ 6 15 ] The extract solution with different concentrations (25–200 μg/mL) was prepared by dissolving the extract using mL methanol p.a. As much as 4 mL dissolved extract was then mixed with 1 mL (0.4 mM), followed by 30 min incubation in a dark condition at 37°C and measured using ultraviolet-visible spectrophotometer (Infinite M200, Tecan, Switzerland) at'.…”

Section: Methodsmentioning

confidence: 99%

“…Cells were grown at a concentration of 5000 cell/100 μL in Dulbecco's modification of Eagle medium, fetal bovine serum (5%), penicillin (100 U/mL), and streptomycin 100 μg/mL at 37°C and 5% CO 2 saturation. [ 6 7 ]…”

Section: Methodsmentioning

confidence: 99%

“…[ 5 ] In this study, we aim to preliminarily investigate the potential of developing a new drug from a natural resource, namely Sphagticola trilobata (L.) J.F Pruski. [ 6 7 8 ]…”

Section: Introductionmentioning

confidence: 99%

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Anticancer, Antioxidant, and Antibacterial Activities of the Methanolic Extract from Sphagneticola trilobata (L.) J. F Pruski Leaves

Mardina

Halimatussakdiah

Harmawan

et al. 2021

Journal of Advanced Pharmaceutical Technology &Amp; Research

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This study aims to investigate the potential of bioactive secondary metabolites contained in Sphagneticola trilobata (L.) J.F Pruski leaves as novel plant-derived anticancer agent. Qualitative bioactive compound contents in the methanolic extract of S. trilobata leaves were screened using phytochemical method. Antioxidant evaluation was carried out using 2,2-diphenyl-1-picrylhydrazyl assay; antibacterial – using well diffusion method on Escherichia coli and Salmonella typhi; and cytotoxicity – using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay on MCF-7 cell line and Vero Cell. It was found that the methanolic extract exhibited antioxidant activity with an IC50 value of 124.34 μg/mL. The inhibition zone values against E. coli and S. thypi (at extract concentration of 100 mg/mL) were 34.33 and 36 mm, respectively. In vitro MTT assay showed that our extract successfully reached 96% mortality with LC50 = 189.287 μg/mL, where the selective index of 2.5 suggest its selectivity against MCF-7 breast cancer cell line. In conclusion, the data of biological activities suggest the potential development of methanolic extract from S. trilobata leaves as a phytomedicine for breast cancer treatment.

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Trilobolide-6-O-isobutyrate from Sphagneticola trilobata acts by inducing oxidative stress, metabolic changes and apoptosis-like processes by caspase 3/7 activation of human lung cancer cell lines

Concato-Lopes,

Gonçalves-Lens,

Tomiotto-Pellissier

et al. 2024

Phytomedicine

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Antioxidant and cytotoxic activities of the ethyl acetate extract of Sphagneticola trilobata (L.) J.F. Pruski on MCF-7 breast cancer cell

Cited by 10 publications

References 18 publications

Effect of Sphagneticola Trilobata Extract on Histological Wistar Rat Kidney Induced by DMBA

Effect of Sphagneticola Trilobata Extract on Histological Wistar Rat Kidney Induced by DMBA

Anticancer, Antioxidant, and Antibacterial Activities of the Methanolic Extract from Sphagneticola trilobata (L.) J. F Pruski Leaves

Trilobolide-6-O-isobutyrate from Sphagneticola trilobata acts by inducing oxidative stress, metabolic changes and apoptosis-like processes by caspase 3/7 activation of human lung cancer cell lines

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