This study was undertaken to measure the physicochemical characteristics, and tyrosinase and elastase inhibitory activity of Sophorae fructus fermented with various useful microorganisms (BS: Bacillus subtilis, CU: Candida utilis, SC: Saccharomyces cerevisiae strain CHY1011, LP: Lactobacillus plantarum, LC: Lactobacillus casei), to investigate the potential for future application of Sophorae fructus as an industrial raw material in health functional foods and cosmetics. The highest yield of freeze-dried powder of fermented Sophorae fructus was obtained with LP. The total phenol content was highest in the group fermented by CU. Maximum flavonoid contents were obtained in the control group, but the group fermented by CU had highest total flavonoid content as compared to other fermented groups. The DPPH radical scavenging activity showed IC50 values of 0.20 mg/mL and 0.18 mg/mL in the control group and the group fermented by BS, respectively, whereas the ABTS radical scavenging activities had IC50 values of 1.15 mg/mL and 0.86 mg/mL for control group and the group fermented by CU, respectively. Highest FRAP value was obtained in the CU group. Tyrosinase inhibition activity was determined to be the highest at 10 mg/mL in the LP fermented group, whereas elastase inhibition was highest at 1 mg/mL in the BS fermented group. Evaluation of the antimicrobial activity revealed inhibition of Staphylococcus aureus growth. Taken together, our results indicate that fermentation of Sophora fructose using various microorganisms has the potential to be developed for use in functional foods and cosmetics, due to the acquired property of increased antioxidant activity and enzyme inhibition.