Antioxidant Activity and Probiotic Properties of Lactic Acid Bacteria

Kim, Seonyoung; Lee, Ji Yeon; Jeong, Yulah; Kang, Chang-Ho

doi:10.3390/fermentation8010029

Cited by 55 publications

(32 citation statements)

References 39 publications

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“…All LAB has DPPH and ABTS radical scavenging activity. Among them, MG5020 had the highest radical scavenging activity, as reported by Kim et al (2022).…”

Section: Resultsmentioning

confidence: 66%

Potential Protective Role of Probiotic Strains of lactobacilli Against Pesticide Toxicity

Palanisamy¹,

Vijila²

2022

MAJ

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Lactic acid bacteria (LAB) are commonly associated with agricultural produces like cereals, fruits, and vegetables. Probiotic lactobacilli are the potential microbes to reduce unavoidable pesticide absorption besides their ability to degrade pesticides in humans and wildlife. The present study aimed to evaluate the strains Lactobacillus plantarum Pb3, Lactobacillus acidophilus Pc1 and Lactobacillus lactis Pt4 for their antioxidant ability and tolerance to simulated gastric and intestinal juice to understand their effects against oxidative damage induced by the pesticides chlorpyriphos, imidacloprid and chlorantraniliprole. Among the three strains, L. plantarum Pb3 exhibited the highest antioxidant ability and tolerance to simulated gastric and intestinal juices, followed by L. acidophilus Pc1 and L. lactis Pt4. Intact cells possessed higher activity than cell-free extracts and cellfree supernatant. The lipid peroxidation inhibition ability of intact cells of L. plantarum Pb3 in the presence of chlorpyriphos, imidacloprid and chlorantraniliprole was 58.12%, 50.75% and 48.88% respectively, and it was 46.19% in the absence of pesticides. Hydroxyl radical scavenging abilities of intact cells of L. plantarum Pb3 in the presence of chlorpyriphos, imidacloprid, and chlorantraniliprole were 52.09%, 49.88%, and 49.15% respectively, as against 45.56% in the absence of pesticides. Under simulated gastric and intestinal juices, a 70 to 75% survival rate was recorded in L. plantarum Pb3. The antioxidant ability of LAB and the tolerance of simulated gastric and intestinal juices indicated the potential protective effects of L. plantarum Pb3 against the adverse effect of pesticides on human health.

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“…All LAB has DPPH and ABTS radical scavenging activity. Among them, MG5020 had the highest radical scavenging activity, as reported by Kim et al (2022).…”

Section: Resultsmentioning

confidence: 66%

Potential Protective Role of Probiotic Strains of lactobacilli Against Pesticide Toxicity

Palanisamy¹,

Vijila²

2022

MAJ

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“…Another in vivo study reported clinical and animal model validation that a mixture of Lactobacillus and Bifidobacterium could significantly lower postoperative inflammation, enhance immunity, resume gut microbiota composition, and promote postoperative recovery [ 47 ]. According to previous studies, paraprobiotic bacterial strains including B. bifdum MG731, L. casei MG311, L. rhamnosus MG316, S. thermophilus MG5140, etc., had no cytotoxic to RAW264.7 cells, and especially B. bifdum MG731, which had the highest SCFA content among 17 bacterial strains, has high antioxidant activity and anti-inflammatory effects, showing the potential of functional paraprobiotics [ 48 , 49 ]. We speculated that the anti-tumor effects of heat-killed MG731 exhibit gastric cancer growth inhibitory effects via molecules involved in SCFAs, lipoteichoic acid, mannoprotein, and fimbriae.…”

Section: Discussionmentioning

confidence: 99%

Anti-Tumor Effect of Heat-Killed Bifidobacterium bifidum on Human Gastric Cancer through Akt-p53-Dependent Mitochondrial Apoptosis in Xenograft Models

Kim

Choi

Kang

et al. 2022

IJMS

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Paraprobiotics, inactivated microbial cells, regulate immune system and exhibit antioxidant and anti-inflammatory activities in patients with weakened immunity or the elderly. This study evaluated the anti-tumor effects of heat-killed Bifidobacterium and Lactobacillus on human gastric cancer MKN1 cells in vitro and in vivo in xenograft animal models. First, cytotoxicity and apoptosis in MKN1 cells of 11 different heat-killed Bifidobacterium or Lactobacillus strains were examined using the MTT assay or flow cytometry, respectively. Then, BALB/c nude mice xenograft animal models were implanted with human gastric cancer MKN1 cells and orally administered a selected single or a mixture of heat-killed bacterial strains to investigate their inhibitory effect on tumor growth. In addition, the expression of p-Akt, p53, Bax, Bak, cleaved caspase-9, -3, and PARP in the tumor tissues was analyzed using Western blotting assay or immunohistochemistry staining. The results show that heat-killed B. bifidum MG731 (MG731), L. reuteri MG5346 (MG5346), and L. rhamnosus MG5200 (MG5200) induced relatively greater apoptosis than other strains in MKN1 cells. Oral administration of a single dose or a mixture of MG731, MG5346, or MG5200 significantly delayed tumor growth, and MG731 had the most effective anti-tumor effect in the xenograft model. Protein expression of p-Akt, p53, Bax, cleaved caspase-3 and -9, and PARP in tumors derived from the xenograft model correlated with the results of the immunohistochemistry staining.

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“…Adherence of probiotics to HT-29 colorectal cells was analyzed according to the protocol reported in a previous study [20]. Briefly, 1 mL of 1 × 10 6 HT-29 colorectal cells were grown in 12-well plates at 37 • C in an atmosphere containing 5% CO 2 until a cell monolayer was formed.…”

Section: Adherence To Ht-29 Colorectal Cellsmentioning

confidence: 99%

Improvements in Human Keratinocytes and Antimicrobial Effect Mediated by Cell-Free Supernatants Derived from Probiotics

Lee¹,

Kim²,

Kim

et al. 2022

Fermentation

Self Cite

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The skin acts as a physical and physiological barrier, thereby protecting the body from various environmental components and stimuli. Cell-free supernatants (CFS) derived from probiotics can improve skin functions and retain moisture. In this study, to assess the efficacy of CFS derived from Ligilactobacillus salivarius and Limosilactobacillus fermentum, we investigated the barrier strengthening and moisturizing effects of CFS in keratinocytes along with their antibacterial effects. We also determined the adhesive effects of probiotics on colorectal cells. To confirm improvements in moisturization and barrier function mediated by CFS in keratinocytes, hyaluronic acid (HA) production, and mRNA expression of HA synthases (HAS)2, HAS3, and FLG were measured. The results showed that CFS from L. salivarius MG242 and L. fermentum MG901 increased the expression of these genes along with the production of HA (2.40- and 1.95-fold of control). Additionally, CFS derived from L. salivarius MG242 and L. fermentum MG901 inhibited the growth of S. aureus and E. coli, thereby demonstrating inhibitory effects against harmful pathogens observed on the skin. These results indicate that the use of CFS derived from L. salivarius MG242 and L. fermentum MG901 may increase moisturization in the skin and improve barrier function of keratinocytes along with elimination of potential pathogens.

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Antioxidant Activity and Probiotic Properties of Lactic Acid Bacteria

Cited by 55 publications

References 39 publications

Potential Protective Role of Probiotic Strains of lactobacilli Against Pesticide Toxicity

Potential Protective Role of Probiotic Strains of lactobacilli Against Pesticide Toxicity

Anti-Tumor Effect of Heat-Killed Bifidobacterium bifidum on Human Gastric Cancer through Akt-p53-Dependent Mitochondrial Apoptosis in Xenograft Models

Improvements in Human Keratinocytes and Antimicrobial Effect Mediated by Cell-Free Supernatants Derived from Probiotics

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