Potential Protective Role of Probiotic Strains of lactobacilli Against Pesticide Toxicity

Abstract

Lactic acid bacteria (LAB) are commonly associated with agricultural produces like cereals, fruits, and vegetables. Probiotic lactobacilli are the potential microbes to reduce unavoidable pesticide absorption besides their ability to degrade pesticides in humans and wildlife. The present study aimed to evaluate the strains Lactobacillus plantarum Pb3, Lactobacillus acidophilus Pc1 and Lactobacillus lactis Pt4 for their antioxidant ability and tolerance to simulated gastric and intestinal juice to understand their effects against oxidative damage induced by the pesticides chlorpyriphos, imidacloprid and chlorantraniliprole. Among the three strains, L. plantarum Pb3 exhibited the highest antioxidant ability and tolerance to simulated gastric and intestinal juices, followed by L. acidophilus Pc1 and L. lactis Pt4. Intact cells possessed higher activity than cell-free extracts and cellfree supernatant. The lipid peroxidation inhibition ability of intact cells of L. plantarum Pb3 in the presence of chlorpyriphos, imidacloprid and chlorantraniliprole was 58.12%, 50.75% and 48.88% respectively, and it was 46.19% in the absence of pesticides. Hydroxyl radical scavenging abilities of intact cells of L. plantarum Pb3 in the presence of chlorpyriphos, imidacloprid, and chlorantraniliprole were 52.09%, 49.88%, and 49.15% respectively, as against 45.56% in the absence of pesticides. Under simulated gastric and intestinal juices, a 70 to 75% survival rate was recorded in L. plantarum Pb3. The antioxidant ability of LAB and the tolerance of simulated gastric and intestinal juices indicated the potential protective effects of L. plantarum Pb3 against the adverse effect of pesticides on human health.

Probiotic bacteria alleviate chlorpyrifos‐induced rat testicular and renal toxicity: A possible mechanism based on antioxidant and anti‐inflammatory activity

“…In recent years, both in vitro and in vivo studies have indicated that the antioxidant properties of probiotics are mediated by reducing oxidative damage and the ROS rate in human cells 31,55 . Based on our results, exposure to CPF significantly altered renal and testicular antioxidant enzyme capacity that, of course, was completely blocked by treatment with L. casei , L. rhamnosus and L. plantarum .…”

“…53,54 In recent years, both in vitro and in vivo studies have indicated that the antioxidant properties of probiotics are mediated by reducing oxidative damage and the ROS rate in human cells. 31,55 Based on our results, exposure to CPF significantly altered renal and testicular antioxidant enzyme capacity that, of course, was completely blocked by treatment with L. casei, L. rhamnosus and L. plantarum. Previous experimental and clinical studies have emphasized that probiotic supplements have the potential to improve antioxidant capacity in diabetic patients and rats by increasing SOD activity.…”

“…Many studies have revealed the capacity of LAB to reduce the amount of CPF in fermented foods such as pickled Chinese cabbage, 28 Sauerkraut 29 and fermented milk, 30 which can be a suggestion to reduce the toxicity of residual CPF in the body. Furthermore, the potential of probiotics in reducing oxidative stress and the accumulation of free radicals, 31 relieving inflammatory responses 32 and improving the function of the immune system 19 can make them a suitable option for reducing the adverse effects of CPF in body tissues.…”

“…Furthermore, the potential of probiotics in reducing oxidative stress and the accumulation of free radicals, 31 relieving inflammatory responses 32 and improving the function of the immune system 19 can make them a suitable option for reducing the adverse effects of CPF in body tissues.…”