The root of Smilax china L. is used in traditional Korean medicine. We found that the Smilax china L. root extract has strong antimicrobial activity against two Cutibacterium acnes strains (KCTC 3314 and KCTC 3320). The aim of this study was to identify the beneficial properties of Smilax china L. extracts for their potential use as active ingredients in cosmetics for the treatment of human skin acne. The high-performance liquid chromatography (HPLC) and liquid chromatography-hybrid quadrupole time-of-flight mass spectrometry (LC/QTOF/MS) methods were used to obtain the profile of secondary metabolites from the ethyl acetate-soluble fraction of the crude extract. Agar diffusion and resazurin-based broth microdilution assays were used to evaluate antimicrobial activity and minimum inhibitory concentrations (MIC), respectively. Among the 24 metabolites, quercetin, resveratrol, and oxyresveratrol were the most potent compounds against Cutibacterium acnes. Minimum inhibitory concentrations of quercetin, resveratrol, and oxyresveratrol were 31.25, 125, and 250 μg/mL, respectively.
The skin acts as a physical and physiological barrier, thereby protecting the body from various environmental components and stimuli. Cell-free supernatants (CFS) derived from probiotics can improve skin functions and retain moisture. In this study, to assess the efficacy of CFS derived from Ligilactobacillus salivarius and Limosilactobacillus fermentum, we investigated the barrier strengthening and moisturizing effects of CFS in keratinocytes along with their antibacterial effects. We also determined the adhesive effects of probiotics on colorectal cells. To confirm improvements in moisturization and barrier function mediated by CFS in keratinocytes, hyaluronic acid (HA) production, and mRNA expression of HA synthases (HAS)2, HAS3, and FLG were measured. The results showed that CFS from L. salivarius MG242 and L. fermentum MG901 increased the expression of these genes along with the production of HA (2.40- and 1.95-fold of control). Additionally, CFS derived from L. salivarius MG242 and L. fermentum MG901 inhibited the growth of S. aureus and E. coli, thereby demonstrating inhibitory effects against harmful pathogens observed on the skin. These results indicate that the use of CFS derived from L. salivarius MG242 and L. fermentum MG901 may increase moisturization in the skin and improve barrier function of keratinocytes along with elimination of potential pathogens.
Nowadays micro-dust is a serious problem in Korea. In particular micro-dust contains heavy metals such as Pb (lead) and Cd (cadmium) which negatively effect on human health. In this study, we intended to isolate lactic acid bacteria which can scavenge the heavy metals. Firstly we isolated two lactic acid bacteria which were resistant to Ag, Cu, and Zn (30-100 mM AgNO 3, CuSO 4, ZnSO 4 in MRS broth). The two lactic acid bacteria CJNU 1877 and JG 15 were identified Lactobacillus paracasei and Enterococcus faecium, respectively. Subsequently the strains were inoculated in MRS broth and agar plate where 100 ppm of Pb(NO 3 ) 2 and CdSO 4 were added, respectively. They did grow in the conditions and we found aggregations from 24 h cultures, indicating the strains can absorb the heavy metals, which was further proved by scanning electron microscopy (SEM) and Inductively Coupled Plasma-Optical Emission Spectrometer. Therefore the isolated lactic acid bacteria can be used as a probiotics harboring heavy metal scavenging activity.
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