Antioxidant Activity and Lipoxygenase Enzyme Inhibition Assay with Total Flavonoid Assay of Garcinia porrecta Laness. Stem Bark Extracts

Abstract

Introduction:The genus Garcinia which is rich of secondary metabolites, mainly flavonoids, have known to have antioxidant and anti-inflammatory activity through the inhibition of lipoxygenase. There isn't found literature indicating research on inhibition of lipoxygenase activity been done in this plant. The purpose of this study is to obtain the data and determine the potential antioxidant activity, and inhibition of lipoxygenase activity of Garcinia porrecta Laness. stem bark extracts. Methods: This research is included FRAP (Ferric Reducing Antioxidant Power) method antioxidant assay, in vitro lipoxygenase inhibition assay, flavonoids qualitative analysis by thin layer chromatography, and total flavonoids assay in the most active extract. Results: The results showed the methanol, ethyl acetate and n-hexane extracts of G. porrecta Laness. stem bark using FRAP method, has antioxidant activity with EC50 values respectively 1.33; 4.97; and 19.96 µg/mL and lipoxygenase inhibition activity with IC50 values 0.23; 0.52; and 4.87 µg/mL. The most active extract in the both assay is methanol extract which has total flavonoids of 5.66 mg QE/g (quercetin equivalent). Conclusion: The results from the study show extracts of the stem bark of G. porrecta Laness. has antioxidant activity and potential for lipoxygenase inhibition.

Lipoxygenase Inhibition by Plant Extracts

“…Potential lipoxygenase inhibition was investigated for extract of Garcinia plants (Table 3). Sari and Elya performed LOX inhibition assay on the stem bark extracts of Garcinia porrecta Laness [34]. Extraction was conducted in different organic solvents (methanol, n-hexane, and ethyl acetate).…”

Standardization and a-Glycosidase Inhibition of Extracts and Nanoparticle Extract of Kembang Bulan Leaves (Tithonia Diversivolia (Hamsley) A. Gray)

“…The absorption was measured with a UV-Vis spectrophotometer at a wavelength of 371.0 nm. The total flavonoid in vitro antidiabetic activity by using inhibition of alpha-glucosidase enzyme activity was d content of 70% ethanol extract of KBL is expressed in mg Quercetin Equivalent/gram extract (mg QE/g) [11].…”

HPLC–PDA Polyphenolic Quantification, UHPLC–MS Secondary Metabolite Composition, and In Vitro Enzyme Inhibition Potential of Bougainvillea glabra

“…A weak inhibition was recorded by all the extracts in the case of the α-amylase enzyme, and both DCM extracts showed higher inhibition compared to their respective MeOH extracts. Lipoxygenase is involved in inflammation, particularly in the biochemical processes of leukotrienes, which are primary triggers for allergic reactions and inflammation [19]. For the lipoxygenase inhibition assay, the aerial MeOH extract followed by the flower DCM extract shower the highest percentage inhibitions with values of 35.4% and 24.3%, respectively.…”