Antimicrobial substances from the rare actinomycete Nonomuraea rhodomycinica NR4-ASC07^T

Abstract

Nonomuraea rhodomycinica NR4-ASC07 is a rare actinomycete isolated from soil in Sirindhorn peat swamp forest. The crude extract of its culture broth exhibited antimicrobial and anticancer against diverse human pathogens and cancer cells. The chemical investigation of the crude extract led to the isolation of two new metabolites named nonomuric acid (1) and 3-hydroxy deoxydaunorubicinol aglycone (2), along with two known bioactive compounds [ε-rhodomycinone (3) and 7-deoxy-13-dihydrocarminomycinone (4)]. Compounds 1 and 3 showed antimalarial activity with the half maximal inhibitory concentration (IC) of 8.00 and 8.88 μg mL, respectively. Compound 4 inhibited growth of Mycobacterium tuberculosis and Bacillus cereus at the minimum inhibitory concentrations of 50.0 and 12.50 μg mL, respectively. Every compound exhibited cytotoxicity against cancer cells tested at IC ≥ 6.34 μg mL. These finding are the first report of bioactive metabolites produced by strain NR4-ASC07, suggesting that rare actinomycetes are yet promising sources for novel drug discovery.

The secondary metabolites of rare actinomycetes: chemistry and bioactivity

“…Meanwhile, compound 103 displayed inhibitory activity against Mycobacterium tuberculosis and Bacillus cereus with MIC values of 50.0 and 12.50 mg mL À1 , respectively. 32 Madurahydroxylactone 104, as a new inhibitor of Staphylococcus aureus FtsZ, and its derivatives 105-108 have been obtained from Nonomuraea sp. AN100570 from soil collected near Gongju city, Korea.…”

New Insights into cytotoxic metabolites produced by Streptomyces griseus isolate KJ623766: Large scale production, structure elucidation and biological activities

“…These results matched with that of Tsuji et al [35] which showed that γ-rhodomycinone, had no differentiation inducing activity, but was cytotoxic however, this cytotoxicity activity against normal cell lines was much lower than cancer cell lines. Supong and his co works recently showed that εrhodomycinone produced by a rare actinomycete Nonomuraea rhodomycinica NR4-ASC07 has a potential antimalarial activity [36]. Further studies should be conducted to explore the biosynthetic pathways of the respective metabolites as well as to produce new derivatives of the anthracycline family with higher antitumor activities and lower side effects.…”

New Insights into cytotoxic metabolites produced by Streptomyces griseus isolate KJ623766: Large scale production, structure elucidation and biological activities

“…These results matched with that of Tsuji et al [35] which showed that γ-rhodomycinone, had no differentiation inducing activity, but was cytotoxic however, this cytotoxicity activity against normal cell lines was much lower than cancer cell lines. Supong and his coworks recently showed that εrhodomycinone produced by a rare actinomycete Nonomuraea rhodomycinica NR4-ASC07 has a potential antimalarial activity [36]. Further studies should be conducted to explore the biosynthetic pathways of the respective metabolites as well as to produce new derivatives of the anthracycline family with higher antitumor activities and lower side effects.…”