The secondary metabolites of rare actinomycetes: chemistry and bioactivity

Ding, Ting; Yang, Luo-Jie; Zhang, Weidong; Shen, Yun-Heng

doi:10.1039/c9ra03579f

Cited by 60 publications

(38 citation statements)

References 105 publications

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“…It is generally accepted that rare actinobacterial genera possess more potential in terms of secondary metabolites novelty ( Schorn et al, 2016 ; Bundale et al, 2019 ; Ding et al, 2019 ). Thus, exploration of bioactive chemicals from rare actinobacterial strains will be more promising and efficient.…”

Section: Discussionmentioning

confidence: 99%

Bioprospecting of Soil-Derived Actinobacteria Along the Alar-Hotan Desert Highway in the Taklamakan Desert

Liu

Wang

et al. 2021

Front. Microbiol.

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Taklamakan desert is known as the largest dunefield in China and as the second largest shifting sand desert in the world. Although with long history and glorious culture, the Taklamakan desert remains largely unexplored and numerous microorganisms have not been harvested in culture or taxonomically identified yet. The main objective of this study is to explore the diversity, novelty, and pharmacological potential of the cultivable actinomycetes from soil samples at various sites along the Alar-Hotan desert highway in the Taklamakan desert. A total of 590 actinobacterial strains were recovered by the culture-dependent approach. Phylogenetic analysis based on 16S ribosomal RNA (rRNA) gene sequences unveiled a significant level of actinobacterial diversity with 55 genera distributed in 27 families of 12 orders. Thirty-six strains showed relatively low 16S rRNA similarities (<98.65%) with validly described species, among which four strains had already been characterized as novel taxa by our previous research. One hundred and forty-six actinobacterial isolates were selected as representatives to evaluate the antibacterial activities and mechanism of action by the paper-disk diffusion method and a double fluorescent protein reporter “pDualrep2” system, respectively. A total of 61 isolates exhibited antagonistic activity against the tested “ESKAPE” pathogens, among which seven strains could produce bioactive metabolites either to be able to block translation machinery or to induce SOS-response in the pDualrep2 system. Notably, Saccharothrix sp. 16Sb2-4, harboring a promising antibacterial potential with the mechanism of interfering with protein translation, was analyzed in detail to gain deeper insights into its bioactive metabolites. Through ultra-performance liquid chromatography (UPLC)-quadrupole time-of-flight (QToF)-MS/MS based molecular networking analysis and databases identification, four families of compounds (1–16) were putatively identified. Subsequent bioassay-guided separation resulted in purification of four 16-membered macrolide antibiotics, aldgamycin H (8), aldgamycin K (9), aldgamycin G (10), and swalpamycin B (11), and their structures were elucidated by HR-electrospray ionization source (ESI)-MS and NMR spectroscopy. All compounds 8–11 displayed antibacterial activities by inhibiting protein synthesis in the pDualrep2 system. In conclusion, this work demonstrates that Taklamakan desert is a potentially unique reservoir of versatile actinobacteria, which can be a promising source for discovery of novel species and diverse bioactive compounds.

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Section: Discussionmentioning

confidence: 99%

Bioprospecting of Soil-Derived Actinobacteria Along the Alar-Hotan Desert Highway in the Taklamakan Desert

Liu

Wang

et al. 2021

Front. Microbiol.

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“…More attentions have been diverted to microorganisms that are previously inadequately assessed or from underexplored environments. The rare actinomycetes are referred to the actinomycetes strains less frequently obtained than that of commonly isolated Streptomyces spp., even though they might not actually be uncommon in the environment . Among rare actinomycete, the genera Micromonospora was observed to produce the highest numbers of natural products …”

Section: Introductionmentioning

confidence: 99%

Secondary Metabolites from Marine Micromonospora: Chemistry and Bioactivities

Gui

et al. 2020

Chemistry & Biodiversity

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Marine Micromonospora was revealed to be a rather untapped and a rich source of chemically diverse and unique bioactive natural products. This review is aimed to make a comprehensive survey of secondary metabolites that were derived from marine Micromonospora including chemical diversity and biological activities. A total of 116 compounds from 41 marine Micromonospora species have been reported, covering the literatures from 1997 to 2019. These compounds contain several structural classes such as polyketides (PKS), nonribosomal peptides (NRPS), PKS-NRPS hybrids, terpenes and others, and they present cytotoxic, antibacterial, antiparasitic, chemopreventive or antioxidant activities.

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“…In the past decades, actinobacteria, particularly from the genus Streptomyces, have been reported to produce about two thirds of the naturally derived antibiotics in current clinical use, as well as many anticancer compounds [2]. While Streptomyces species appear to have been exhaustively explored [3,4], other genera belonging to the so-called "rare actinobacteria" may still serve as promising sources for novel biologically active secondary metabolites [4][5][6][7]. One of these genera is Nonomuraea, which has recently been reported to produce several new biologically active compounds such as the antimicrobial hypogeamycins B-D [8], nonomuric acid and 3-hydroxy deoxydaunorubicinol aglycone [9], the cytotoxic hypogeamycin A [8], and karamomycins [10].…”

Section: Introductionmentioning

confidence: 99%

Nonocarbolines A–E, β-Carboline Antibiotics Produced by the Rare Actinobacterium Nonomuraea sp. from Indonesia

et al. 2020

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During the course of our ongoing screening for novel biologically active secondary metabolites, the rare Actinobacterium, Nonomuraea sp. 1808210CR was found to produce five unprecedented β-carboline derivatives, nonocarbolines A–E (1–5). Their structures were elucidated from high-resolution mass spectrometry, 1D and 2D nuclear magnetic resonance spectroscopy, and the absolute configuration of 4 was determined by using the modified Mosher method. Nonocarboline B (2) displayed moderate antifungal activity against Mucor hiemalis, while nonocarboline D (4) exhibited significant cytotoxic activity against the human lung carcinoma cell line A-549 with the IC50 value of 1.7 µM.

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The secondary metabolites of rare actinomycetes: chemistry and bioactivity

Abstract: A detailed introduction to the structures and bioactivities of secondary metabolites from rare actinomycetes is made.

Cited by 60 publications

References 105 publications

Bioprospecting of Soil-Derived Actinobacteria Along the Alar-Hotan Desert Highway in the Taklamakan Desert

Bioprospecting of Soil-Derived Actinobacteria Along the Alar-Hotan Desert Highway in the Taklamakan Desert

Secondary Metabolites from Marine Micromonospora: Chemistry and Bioactivities

Nonocarbolines A–E, β-Carboline Antibiotics Produced by the Rare Actinobacterium Nonomuraea sp. from Indonesia

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