Antimicrobial resistance in fecal Escherichia coli isolated from growing chickens on commercial broiler farms

Ozaki, Hiroichi; Esaki, Hidetake; Takemoto, Kouhei; Ikeda, A.; Nakatani, Yasutaka; Someya, Azusa; Hirayama, Norio; Murase, Toshiyuki

doi:10.1016/j.vetmic.2010.12.020

Cited by 27 publications

(26 citation statements)

References 20 publications

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“…Similar to our results, increase in the numbers of resistance phenotypes during the growth of the chickens and change in faecal microbiome was established by Ozaki et al (2011). These authors showed that the change can occur even in the absence of antimicrobial administration.…”

Section: Discussionsupporting

confidence: 91%

Changes in antimicrobial resistance patterns and dominance of extended spectrum β-lactamase genes among faecal Escherichia coli isolates from broilers and workers during two rearing periods

Doregiraee

Alebouyeh

Fasaei

et al. 2017

Italian Journal of Animal Science

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The emergence of antibiotic-resistant Escherichia coli, especially extended-spectrum b-lactamase (ESBL)-producing strains, in the intestinal tract of broilers could be a threat to poultry and human. We investigated changes of antimicrobial resistance patterns and frequency of ESBL genes among faecal E. coli isolates of broilers and workers in five different farms during two rearing periods in Iran. In this regard, E. coli was isolated from rectal swabs of the workers and cloacal swabs of the broilers. After detection of antibiotic resistance patterns, phenotypic and genotypic characterisation of ESBL phenotype in these strains, carriage of the resistance genes on their crude plasmid extracts and diversity of plasmid profiles were analysed. Accordingly, multidrug-resistant (MDR) patterns were detected in a high percentage of E. coli strains from the workers (72.7%) and poultry (92.3%). ESBL-producing E. coli strains were identified in these farms throughout the two periods of rearing (6.3%). bla CTX-M-1 , bla CTX-M-61 , bla TEM-116 and bla TEM-1 were characterised in 6 (Period I/II: 4/2), 1 (Period I), 2 (Period II) and 9 (Period I/II: 5/4) strains, respectively. The first isolation of E. coli strains harbouring the bla TEM116 gene in chicken is reported in this study. In conclusion, results of this study showed that chickens could serve as a reservoir for ESBL-producing E. coli strains. These strains could carry clinically important ESBL or new emerging b-lactamases genes. Early colonisation and selection of the resistant strains during rearing periods proposed illegal use of antimicrobials as the cause of change in resistance patterns in the studied farms. ARTICLE HISTORY

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Section: Discussionsupporting

confidence: 91%

Changes in antimicrobial resistance patterns and dominance of extended spectrum β-lactamase genes among faecal Escherichia coli isolates from broilers and workers during two rearing periods

Doregiraee

Alebouyeh

Fasaei

et al. 2017

Italian Journal of Animal Science

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show abstract

“…Conversely, common resistance profiles and integrons were shared among isolates belonging to different PFGE types. This difference in antimicrobial susceptibility may be explained by horizontal transmission of resistance determinants among strains, as documented previously by Ozaki et al (2011). Further research is needed to ascertain the localization of integrons either on chromosome or plasmids in order to understand their contribution to the dissemination of resistance.…”

Section: Discussionmentioning

confidence: 78%

Antimicrobial resistance and class 1 and 2 integrons inEscherichia colifrom meat turkeys in Northern Italy

Piccirillo¹,

Giovanardi²,

Dotto³

et al. 2014

Avian Pathology

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This study is aimed at determining the antimicrobial resistance (AMR) and the presence of class 1 and 2 integrons in 48 avian pathogenic Escherichia coli (APEC) strains isolated from meat turkeys during three sequential production cycles. Thirty avian faecal E. coli (AFEC) strains from the first cycle were also analysed. Strains were tested for AMR against 25 antimicrobials by disk diffusion test and were screened for the presence of integrons and associated gene cassettes by polymerase chain reaction followed by sequencing. Genetic relatedness of isolates was established by pulsed-field gel electrophoresis. High levels of resistance were detected to tetracyclines, penicillins and sulphonamides in APEC and AFEC. Resistance to aminoglycosides, fluoroquinolones, cephalosporins and phenicols was variable, based on the antimicrobial drug and the isolate (APEC vs. AFEC). Full susceptibility to colistin was detected. Multidrug resistance of up to seven antimicrobial classes was exhibited by APEC (93.8%) and AFEC (100%). Nearly 44% of strains tested positive for class 1 and/or class 2 integrons containing the dfrA, aadA and sat2 genes, alone or in combination, coding for streptomycin/spectinomycin, trimethoprim and streptothricin resistance, respectively. The estX and orfF genes of unknown function were also detected. A significant association was found between the presence of integrons and the resistance to aminoglycosides and potentiated sulphonamides. The results of this study showed that AMR, multidrug resistance and class 1 and 2 integrons are widespread among pathogenic and commensal E. coli from Italian turkeys. More attention should be addressed to limit the use of antimicrobials in turkeys and the AMR of turkey E. coli.

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“…Our results indicate a generally higher or similar prevalence of AMR among chicken E. coli isolates from Vietnam to commonly used antimicrobials (tetracycline, chloramphenicol, ampicillin and gentamicin) compared with results from industrialized countries. 34–36 Data from seven European countries suggest a higher prevalence of ciprofloxacin resistance (57.6%), while data from five European countries indicate a higher prevalence of ceftazidime resistance (11.1%) in chickens in these countries. 37 Although such comparisons should be interpreted with caution because of differences in sampling methods as well as differences in the breakpoints used for interpreting susceptibility test results between studies from different regions, the high prevalence of AMR observed in these backyard farms in Vietnam is striking and unexpected.…”

Section: Discussionmentioning

confidence: 99%

Prevalence and risk factors for carriage of antimicrobial-resistant Escherichia coli on household and small-scale chicken farms in the Mekong Delta of Vietnam

Nguyen

Carrique-Mas

Ngo

et al. 2015

Journal of Antimicrobial Chemotherapy

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Antimicrobial resistance in fecal Escherichia coli isolated from growing chickens on commercial broiler farms

Cited by 27 publications

References 20 publications

Changes in antimicrobial resistance patterns and dominance of extended spectrum β-lactamase genes among faecal Escherichia coli isolates from broilers and workers during two rearing periods

Changes in antimicrobial resistance patterns and dominance of extended spectrum β-lactamase genes among faecal Escherichia coli isolates from broilers and workers during two rearing periods

Antimicrobial resistance and class 1 and 2 integrons inEscherichia colifrom meat turkeys in Northern Italy

Prevalence and risk factors for carriage of antimicrobial-resistant Escherichia coli on household and small-scale chicken farms in the Mekong Delta of Vietnam

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