Antimicrobial resistance and class 1 and 2 integrons in<i>Escherichia coli</i>from meat turkeys in Northern Italy

Piccirillo, Alessandra; Giovanardi, Davide; Dotto, Giorgia; Grilli, G.; Montesissa, Clara; Boldrin, Caterina; Salata, Cristiano; Giacomelli, M.

doi:10.1080/03079457.2014.943690

Cited by 16 publications

(16 citation statements)

References 42 publications

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“…The data presented here shows that class 2 integrons were less frequently detected 5 (2.8%). Similar data have been published for Enterobacteriaceae in Nigeria [36, 38] and elsewhere [34, 39, 40]. Ramírez et al [39], reported that unlike the widespread distribution of class 1 integron within Gram-negative bacilli, only Acinetobacter baumannii and Enterobacter cloacae harboured class 2 integrons at a high frequency.…”

Section: Resultssupporting

confidence: 73%

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Multidrug resistance and high prevalence of class 1 integrons inEscherichia coliisolated from waters and vegetables in Nsukka and Enugu, Nigeria

Chigor

Ibangha

Nweze

et al. 2020

Preprint

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41In spite of treated wastewater presenting itself as an attractive alternative to scarce quality water 42 in the developing countries, the associated contamination of fresh produce by irrigation waters 43 leading to outbreak of foodborne illnesses is on the rise. Horizontal transfer of integrons play 44 important role in the spread and maintenance of antimicrobial resistance among strains of 45 Escherichia coli. This study assessed the effluents from the University of Nigeria, Nsukka 46 Wastewater Treatment Plant (UNN-WWTP) as well as vegetables irrigated with the effluent, and 47 vegetables sold in selected markets from Nsukka and Enugu cities for the presence of E. coli and 48 determined the prevalence integrons in multidrug-resistant isolates. Isolation of E. coli was done 49 using eosin methylene blue agar and isolates subjected to Gram staining for identification of 50 presumptive colonies. Confirmation of E. coli was achieved by polymerase chain reaction (PCR) 51 technique, targeting beta-glucuronidase (uidA). Resistance to antibiotics was determined using the 52 Bauer-Kirby disk diffusion assay and the Clinical and Laboratory Standard Institute criteria.53Integrons were detected by multiplex PCR using primers specific for class 1 and 2 integrons. A 54 total of 178 E. coli isolates were obtained from WWTP effluent (41), and vegetables from 55 greenhouse (46), farms (55) and market (36). Multi-drug resistance was detected in all the isolates, 56 ranging from five-drug resistance in a single isolate to 16-drug resistance patterns in two different 57 isolates. Of the total isolates, class 1 integrons were abundantly detected in 175 (98.3%) and class 58 2 in 5 (2.8%). All the class 2 integrons were found in isolates that were positive for class 1. The 59 high detection of E. coli in the studied effluent and vegetables pose potential public health hazards 60 heightened by observed multidrug resistance in all the isolates and the high prevalence of class 1 61 integron. It is concluded that the vegetable samples are significant reservoirs for potentially 62 pathogenic E. coli. Therefore, vegetable irrigation farming with unsafe water should be 63 discontinued, while appropriate improvement strategies to ensure compliance should be facilitated 64 without further delay. 65 66 Pathogenic Escherichia coli causes significant morbidity and mortality worldwide [1-3]. Reported 68 risk factors in the developing countries and sub-Saharan African regions include poor hygiene, 69 unsafe water, improper disposal of waste and faeces, and contaminated food, local beverages and 70 vegetables [2, 4, 5]. Vegetables can become contaminated with pathogenic and commensal 71 bacteria from animals and humans, during growth, harvesting, distribution, storage and processing 72 [6]. Although the contamination of fresh produce by irrigation waters has led to outbreak of 73 foodborne illnesses, yet treated wastewater presents itself as an attractive alternative to scarce 74 quality water in the developing countries. 75 E. coli has bee...

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Section: Resultssupporting

confidence: 73%

“…All the class 2 integrons were found in isolates that were positive for class 1. Such co-carriage has been previously published on E. coli from meat turkeys in Italy, [34].…”

Section: Resultsmentioning

confidence: 63%

Multidrug resistance and high prevalence of class 1 integrons inEscherichia coliisolated from waters and vegetables in Nsukka and Enugu, Nigeria

Chigor

Ibangha

Nweze

et al. 2020

Preprint

View full text Add to dashboard Cite

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“…Similarly, Marchant et al (2013) have shown resistance against sulphonamides, trimethoprim, streptomycin, chloramphenicol, amoxicillin, tetracycline and neomycin to be associated with presence of integrons. Piccirillo et al (2014) have also suggested the differences between intI1-positive and intI1-negative isolates in resistance to gentamycin, streptomycin, aminosidine, triple sulphonamides and trimethoprim combined with sulfame thoxazole to be significantly higher in intI1-positive compared with intI1-negative isolates. In contrast, Oosterik et al, (2014) have found significant relationship for the presence of Class 1 integrons and resistance to trimethoprim, sulphonamides, and tetracycline only.…”

Section: Detection Of Class 1 Integronsmentioning

confidence: 99%

Risk factors for antimicrobial resistance in Escherichia coli isolates from poultry in Haryana

Kumar

Gupta

2019

IJAR

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Colibacillosis is a disease of severe economic significance to all poultry producers worldwide, characterized by a diverse array of lesions. Due to enormous exploitation of antibiotics in broilers, an increased number of resistant bacterial strains have developed in recent years. 106 E. coli strains were collected from broilers suffering from colibacillosis. The resistance to different classes of antimicrobials was determined. The presence of integrons was determined by Polymerase Chain Reaction (PCR). All the statistical analyses were carried out using STATA™. All the isolates were multidrug resistance (MDR), i.e. resistant to at least one agent in three or more antimicrobial categories. 37 (34.90%) isolates were found positive for Class 1 integrons. There was significant difference in carriage of integrons in different phylogentic groups, p=0.001, however, there was no difference among different serotypes for carriage of integrons. Significant difference was observed for resistance towards co-trimoxazole (p=0.002), piperacillin (p=0.034) and ciprofloxacin (p=0.046) in Class 1 integron carrying isolates and Class 1 integron negative isolates. Significant difference was observed for resistance towards ceftriaxone (p=0.004) and cefotaxime (p=0.027) in relation to phylogenetic groups. Also, significant difference was observed among different serotypes for resistance towards co-trimoxazole (p=0.001), gentamicin (p=0.005) and kanamycin (p=0.041).

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“…Multidrug resistance. MDR is defined as resistance to three or more classes of antimicrobial drugs; it is commonly seen among livestock isolates of E. coli (Cheney et al, 2015;Piccirillo et al, 2014) and S. enterica (European Centre for Disease Prevention and Control, European Food Safety Authority, & European Medicines Agency, 2017;Gomes-Neves et al, 2014;Mueller-Doblies, Clouting, & Davies, 2013) in Europe. The occurrence of MDR in the latter (zoonotic) species is a focus of particular concern.…”

Section: Current Issues For Amr In Livestock Bacteriamentioning

confidence: 99%

Antimicrobial Resistance on Farms: A Review Including Biosecurity and the Potential Role of Disinfectants in Resistance Selection

Davies

Wales

2019

Comp Rev Food Sci Food Safe

120

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Resistance to therapeutic antimicrobial agents is recognized as a growing problem for both human and veterinary medicine, and the need to address the issue in both of these linked domains is a current priority in public policy. Efforts to limit antimicrobial resistance (AMR) on farms have so far focused on control of the supply and use of antimicrobial drugs, plus husbandry measures to reduce infectious disease. In the United Kingdom and some other countries, substantial progress has been made recently against targets on agricultural antimicrobial drug use. However, evidence suggests that resistant pathogenic and commensal bacteria can persist and spread within and between premises despite declining or zero antimicrobial drug use. Reasons for this are likely complex and varied but may include: bacterial adaptations to ameliorate fitness costs associated with maintenance and replication of resistance genes and associated proteins, horizontal transmission of genetic resistance determinants between bacteria, physical transfer of bacteria via movement (of animals, workers, and equipment), ineffective cleaning and disinfection, and co‐selection of resistance to certain drugs by use of other antimicrobials, heavy metals, or biocides. Areas of particular concern for public health include extended‐spectrum cephalosporinases and fluoroquinolone resistance among Enterobacteriaceae, livestock‐associated methicillin‐resistant Staphylococcus aureus, and the emergence of transmissible colistin resistance. Aspects of biosecurity have repeatedly been identified as risk factors for the presence of AMR on farm premises, but there are large gaps in our understanding of the most important risk factors and the most effective interventions. The present review aims to summarize the present state of knowledge in this area, from a European perspective.

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Antimicrobial resistance and class 1 and 2 integrons inEscherichia colifrom meat turkeys in Northern Italy

Cited by 16 publications

References 42 publications

Multidrug resistance and high prevalence of class 1 integrons inEscherichia coliisolated from waters and vegetables in Nsukka and Enugu, Nigeria

Multidrug resistance and high prevalence of class 1 integrons inEscherichia coliisolated from waters and vegetables in Nsukka and Enugu, Nigeria

Risk factors for antimicrobial resistance in Escherichia coli isolates from poultry in Haryana

Antimicrobial Resistance on Farms: A Review Including Biosecurity and the Potential Role of Disinfectants in Resistance Selection

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