Antimicrobial, Anti-inflammatory and antioxidant activities of Jatropha multifida L. (Euphorbiaceae)

Abstract

Background:Jatropha multifida is used in Togolease folk medicine for the healing of chronic wounds.Objective:This study aims to investigate antibacterial, anti-inflammatory and antioxidant activities of the leaves ethanolic extract.Materials and Methods:The antimicrobial activity was assayed by National Committee for Clinical Laboratory Standards broth microdilution method on strains of Staphylococcus aureus and Pseudomoas aeruginosa isolated from wounds, whereas the anti-inflammatory activity was performed by carrageenan and histamine induced paw edema method in rat modele. The 2, 2-diphenyl-1picrylhydrazyl (DPPH) free radical scavenging and ferric reducing antioxidant power (FRAP) were used for the antioxidant activity.Results:The antibacterial assay showed an in vitro growth inhibition of P. aeruginosa and S. aureus in dose-dependent manner, with minimum inhibitory concentration values ranging from 2.5 to 3.12 mg/mL for S. aureus and from 6.25 to 12.5 mg/mL for P. aeruginosa. The maximum paw anti-inflammatory effect occurred after 3 and 5 h administration of histamine and carrageenan, respectively. The DPPH radical scavenging and the FRAP assays yielded weak antioxidant activity.Conclusion:J. multifida possesses antibacterial and anti-inflammatory activities that could justify the use of the plant for the treatment of wounds in the folk medicine.SUMMARY Antibacterial on germs isolated from wound, anti-inflammatory and antioxidant activities of Jatropha multifida were assayed by NCCLS broth method, carrageenan and histamine, DPPH and FRAP respectively. The results indicated that Jatropha multifida possesses antibacterial and anti-inflammatory and weak antioxidant activities that could justify its use for the treatment of wounds in the folk medicine.

Conservation and Variation of 35S Ribosomal DNA among Five <i>Jatropha</i> Species Revealed by Fluorescence <i>In Situ</i> Hybridization

“…However, its genetic variation is limited (Tanya et al 2011, Montes et al 2014, and thus the use of related species for interspecific hybridization is required for genetic improvement (One et al 2014a, One et al 2014b, Fukuhara et al 2016. Several Jatropha species closely related to J. curcas are used as medicinal and ornamental plants (Muakrong et al 2014, Anani et al 2016.…”

The Medicinal Plants Used in Anjir Pulang Pisau, Central Kalimantan-Indonesia

“…The antibacterial assay of Jatropa multifida L. showed an in vitro growth inhibition of P. aeruginosa and S. aureus in dose-dependent manner. 20 Stenochlaena palustris (Burm.f.) Bedd.…”

Antimicrobial Activities of Syzygium aromaticum (L.) Merr. and L.M. Perry (Myrtaceae) Fruit Extracts on Six Standard Microorganisms and Their Clinical Counterpart

“…In fact, the alkaloids, saponosides, flavonoids and tannins in the fruits of the plant are known to have a curative activity against several pathogenic agents: S. aureus, E. coli, P. aeruginosa and C. albicans [19] [24]. The action mechanism of tannins is complexation either with enzymes or with bacterial substrates or with metal ions or its action on the cell membrane of bacteria [18] [25]. Tannins are known to have inhibitory activities on the growth of S. aureus and P. aeruginosa [26].…”

“…Tannins are known to have inhibitory activities on the growth of S. aureus and P. aeruginosa [26]. Flavonoids cause lysis of the membrane and consequently death of the cell [18] [27]. These mechanisms of action of tannins and flavonoids could partly explain the bactericidal and bacteriostatic effects given by the aqueous, hydroethanolic and ethanolic extracts of S. aromaticum on the tested microorganisms.…”