Antimicrobial and Antioxidant Activities of Novel Marine Bacteria (Bacillus 2011SOCCUF3) Isolated from Marine Sponge (Spongia officinalis)

Abstract

Background: Bacillus species represent a rich source of new bioactive metabolites that can combat diseases. Methods: Bacillus strain was isolated from the marine sponge Spongia officinalis and routinely maintained on marine broth. The bacteria strain was identified as Bacillus 2011SOCCUF3 using 16S rDNA sequencing. The strain was cultured on Tryptone Casein Oat Soluble Starch (TCOATSS) media with continuous agitation for 4 days. The fermented broth was centrifuged, and the supernatant was mixed with 10% (w/v) of adsorbent resin (XAD-7HP and XAD-16N, 1:1) and shaken continuously at a reduced speed for 7 h; and the resin was collected by filtration through sintered glass funnel and washed with MilliQ water, and then eluted with methanol to obtain the extract. The extract was evaporated in vacuo at reduced temperature and pressure to obtain the dry extract. The dry extract was purified by vacuum liquid chromatography, eluting with methanol in acetone gradient. The in vitro antimicrobial and antioxidant activities were investigated using the agar-well diffusion, DPPH scavenging and the phosphomolybdate methods respectively. Results: The extract and fractions showed good antimicrobial activities with minimum inhibitory concentration range of <1.0 mg/mL. The extract and fractions also exhibited good antioxidant activities with their IC 50 values been comparable to the standard. Conclusion: Thus, a novel Bacillus strain isolated from the marine sponge (Spongia officinalis) obtained from Cortiou and Riou, France, exhibited promising antimicrobial and antioxidant activities.

Bioprospecting of bacterial symbionts of sponge Spongia officinalis from Savu Sea, Indonesia for antibacterial potential against multidrug-resistant bacteria

“…The antibacterial activity against MDR bacteria belonged to the members of the Bacillus genus. Previous studies on bacteria associated with Spongia have shown similar results (Odekina et al 2020). According to Mondol et al (2013) Bacillus isolated from the sea produces secondary metabolites various antimicrobial activities (Mondol et al 2013).…”

Marine Demospongiae: A Challenging Treasure of Bioactive Compounds

“…The extract of the former strain was efficient against Bacillus subtilis, E. coli, S. aureus, Agrobacterium tumefaciens and the yeast Saccharomyces cerevisiae; while the extract of the latter strain demonstrated activity against B. subtilis, A. tumefaciens, S. aureus and the yeast S. cerevisiae [87]. Frequently, the isolated bacterial species belong to the genus Bacillus, as is the case for the strain "2011SOC-CUF3", isolated from the marine sponge Spongia officinales (Linnaeus, 1759), subfractions of which were active against as S. aureus (MIC = 247 µg/mL), S. tiphy (MIC = 83 µg/mL), P. aeruginosa (MIC = 162 µg/mL) and E. coli (628 µg/mL), compared with the positive controls (ciprofloxacin and fluconazole) [88]. Similarly, from the sponge Halichondria glabrata (Keller, 1891) (collected in Mumbai) the strain GSA10 was isolated and successively classified for its similarities with pG1 Bacillus amyloliquefaciens.…”

“…(2011SOCCUF3), isolated from Spongia officinalis (Linnaeus, 1759), showed a strong antifungal activity. The vacuum liquid chromatography (VLC) fractions obtained from this bacterium showed specific activity against C. albicans (MIC = 108 µg/mL) [88]. Similarly, against C. albicans, a methanol extract from the marine Demospongia Neopetrosia exigua (Kirkpatrick, 1900) showed encouraging activity [77], as well as for the "peptide C" extracted from the deep sea marine sponge S. fortis (MIC = 18.07 µM [81].…”

Extract and Fractions from Soil Bacteria (Streptomyces canus ATCC 12647) Possess Antimicrobial and Anti-Oxidative Potential in vitro

“…Briefly, the dried extract (10.8 g) was triturated with silica gel (10.0 g) in a mortar and loaded onto a sintered glass Buckner funnel (6 cm x 30 cm, ID) attached to a vacuum line and packed with graded silica gel 60 (0.04-0.063 mm, 230-400 mesh) as adsorbent, then eluted with methanol in acetone gradient (25, 50, 75 and 100%, 1 L each) to yield the VLC fractions (F1-F4). These sub-fractions were subsequently concentrated to remove the solvents and used for the antimicrobial and antioxidant studies 13 .…”