Antimicrobial activity of the leaf extract and fractions of Lupinus arboreus

Ohadoma, Sylvester Chika; Nnatuanya, I. O.; Amazu, Louis U; Okolo, C E

doi:10.5897/jmpr12.1263

Cited by 15 publications

(19 citation statements)

References 14 publications

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“…The Gram-positive bacteria (e.g., B. subtilis) are known to be more sensitive to plant active extracts than Gram-negative bacteria (e.g., P. aeruginosa). This finding, as suggested by Ohadoma et al, indicates that the sensitivity to each group of bacteria is based on the fact that the cell wall of Gram-positive bacteria is less complex than other groups and does not have the effect of the natural sieve against large molecules [2]. The results for the MIC are presented in Table 2.…”

Section: Resultssupporting

confidence: 61%

“…The MIC of plant fractions was evaluated by the microdilution method [2]. The fraction solutions were transferred in 80% DMSO, v/v (20,000 ppm, 50 µL) into 96 well plates in the first row.…”

Section: Minimum Inhibitory Concentration (Mic)mentioning

confidence: 99%

“…Microorganisms have unfavorable impact on the quality and safety of life [2]. To against these microorganisms widely used chemicals [2]. The occurrence of drug resistance demands new antimicrobial.…”

Section: Introductionmentioning

confidence: 99%

“…There is the need for intensive studies for the possible discovery of new agents with antibacterial potentials [1]. Microorganisms have unfavorable impact on the quality and safety of life [2]. To against these microorganisms widely used chemicals [2].…”

Section: Introductionmentioning

confidence: 99%

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Antibacterial Activity of Fractions of Ethyl Acetate Extract of Garcinia Lattissima Miq. Fruits

Ambarwati

Malik

Listari

et al. 2017

Asian J Pharm Clin Res

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Objectives: The emergence of new infections and increase in bacterial drug resistance has created a serious need for the expansion of new antibacterial agents from natural sources. The study was carried out to evaluate the antibacterial activity of fractions of ethyl acetate extract of Garcinia latissima Miq. fruits. Methods:The fractionation was done using a silica gel column and organic solvents as the eluent, i.e., n-hexane, ethyl acetate, and methanol. All fractions were assayed for antibacterial activity, which was done by performing disc diffusion for growth inhibition against Bacillus subtilis and Pseudomonas aeruginosa. In addition, the growth inhibition activity was also examined by performing bioautography assay using pre-coated silica gel 60 GF 254 plates as the stationary phase. Fractions A-F were eluted using n-hexane:chloroform (1:4), while Fractions G-K were used ethyl acetate:dichloromethane (4:1) as the mobile phase. The plate was visualized by ultraviolet at λ 254 nm and 366 nm, while the other one was contacted with the inoculated agar medium to observe zone inhibition. Further, the minimum inhibitory concentration (MIC) value was determined by performing microdilution. Results:The result showed that the antibacterial activity of all fractions was more active at inhibiting the growth of B. subtilis than P. aeruginosa, mainly for Fractions H and J. However, the strongest antibacterial activity was showed by Fractions H and J against B. subtilis, MIC 312.5 µg/mL (lower than reference, which is erythromycin antibiotic (25 µg/mL), followed by Fraction D against B. subtilis MIC 625 µg/mL, Fraction K against P. aeruginosa MIC 625 µg/mL, whereas Fractions C, E, and G against B. subtilis, and Fraction E against P. aeruginosa also showed low MIC values (1.250 µg/mL). Conclusions:The results indicated that fractions of G. latissima Miq. fruit ethyl acetate extracts possessed antibacterial activity. The most active fraction that inhibited the growth of B. subtilis was shown by Fractions H and J; these fractions have the potential to be developed as new antibacterial agents.

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Section: Resultssupporting

confidence: 61%

Section: Minimum Inhibitory Concentration (Mic)mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Antibacterial Activity of Fractions of Ethyl Acetate Extract of Garcinia Lattissima Miq. Fruits

Ambarwati

Malik

Listari

et al. 2017

Asian J Pharm Clin Res

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“…One explanation is that the cell walls of Gram-positive bacteria are less complex than Gramnegative bacteria and do not have selectivity for large molecules [16]. The walls of these cells are composed of a thick layer of mucopeptides (peptidoglycan) (40-50% of the bacterial body weight), while the cell walls of Gram-negative bacteria contain layers of fat and peptidoglycan (20% and 5-20% of the body weight, respectively) [17].…”

Section: Aeruginosa)mentioning

confidence: 99%

The Antibacterial Activity of Fractions of Ethyl Acetate Garcinia Latissima Miq. Stem Bark Extracts Against Bacillus Subtilis and Pseudomonas Aeruginosa

Ambarwati

Malik²,

Deborah³

et al. 2017

Asian J Pharm Clin Res

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Objective: This research focuses on the antibacterial activities of fractions of ethyl acetate Garcinia latissima Miq. extracts against Bacillus subtilis and Pseudomonas aeruginosa. Methods:For each bacteria, the values of the inhibitory zone diameter were determined using paper discs method, while the minimum inhibitory concentration (MIC) was determined by microdilution and antibacterial assay was determined by bioautography. Fractionation was carried out using gradient elution with silica gel column as stationary phase successively increasing the separation according to the polarity (using combination of eluents; n-hexane, ethyl acetate, and methanol). Results:To determine the diameter of the inhibitory zone for each fraction, the fractions were diluted with dimethyl sulfoxide until the concentration of each fraction was 20,000 ppm. The results showed that fraction B had no inhibitory zone against B. subtilis. The inhibitory zone diameters of fractions A, C, D, E, F, and G against B. subtilis were 7.600±0.000, 6.767±0.202, 7.950±0.477, 7.883±0.901, and 9.233±0.231 mm, respectively. Only Fraction G had an inhibition zone diameter of 7.200±0.173 mm against P. aeruginosa. The active fractions were tested by contact bioautography using silica gel (60 GF254) plate and hexane:chloroform (2:3) as mobile phase for Fractions A, C, D, E, and F and chloroform:ethyl acetate (4:1) as mobile phase for Fraction G. The thin layer chromatography plates were observed under the ultraviolet light at wavelengths of 254 and 366 nm, while the others plate (using in contact bioautography) placing on agar medium that had been inoculated with the bacteria, so the compounds could be diffuse to the medium. The active compound will appeared inhibitory zones in agar medium. The determination of the MIC values using microdilution with methylthiazol tetrazolium salt showed that fractions of the ethyl acetate extracts of G. latissima Miq. stem bark inhibited the growth of B. subtilis more actively than P. aeruginosa. The highest activity of the fractions against B. subtilis was shown by fraction G, which had a MIC value of 78.125 ppm, followed by fractions F (156.25 ppm), E (312.5 ppm), D (625 ppm), C (1250 ppm), B (2500 ppm), and A (5000 ppm). The highest activity of the fractions against P. aeruginosa was Fraction G (5000 ppm), while the MIC values of other fractions were >5000 ppm. Conclusion:Further investigations should be conducted to obtain the new antibacterial compounds in fractions of ethyl acetate extract of G. latissima Miq. stem bark that effective to inhibit the growth of B. subtilis and P. aeruginosa.

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Chemo‐taxonomic and biological potential of highly therapeutic plant Pedicularis groenlandica Retz. using multiple microscopic techniques

Hameed

Zafar

Ahmad

et al. 2021

Microscopy Res & Technique

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Pedicularis groenlandica is one of the most important medicinal plant of Deosai Plateau (Gilgit-Baltistan) Pakistan. The present study was aim to evaluate the micromorphological features, phytochemical screening and pharmacological potential of P. groenlandica by using multiple microscopic techniques. Six different solvents were used to prepare P. groenlandica extracts. Phytochemical and antioxidant activities were determined calorimetrically. To investigate antidiabetic, α-amylase inhibition assay was performed. Cytotoxicity was tested using brine shrimp assay.Anti-leishmanial via MTT assay. Disc-diffusion assay was used for protein kinase inhibitory, antibacterial and antifungal activities. Palyno-anatomical study showed significant variation for the authentication and correct identification of this highly therapeutic plant by using light and scanning electron microscopic techniques. All extracts were found rich in phytochemicals, significant amount of phenolic and flavonoid contents were found in methanol extract (PGM) 95.78 mg GAE/g and 66.90 mg QE/g. Highest DPPH scavenging potential with IC 50 88.65 μg/mL, total antioxidant capacity (60.33 mg AAE/g sample) and total reducing power (83.97 mg AAE/g) were found for PGM. Disc-diffusion method showed significant antibacterial and antifungal activities. Noticeable growth inhibition in L. tropica was displayed by n-hexane extract (IC 50 112 μg/mL). Brine shrimp with highest LD 50 (67.65 μg/mL) in ethyl-acetate extract. Ethanol extract gives persuasive protein kinase inhibition (26 mm) against Streptomyces 85-E hyphae. Highest alphaamylase inhibition (74.10%) was found in n-hexane extract. In conclusion, our findings scientifically support the ethno-medicinal and biological potential of P. groenlandica. In future, the plant needs to be explored for further identification and isolation of bioactive compounds to develop new drugs to treat several aliments.

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Antimicrobial activity of the leaf extract and fractions of Lupinus arboreus

Cited by 15 publications

References 14 publications

Antibacterial Activity of Fractions of Ethyl Acetate Extract of Garcinia Lattissima Miq. Fruits

Antibacterial Activity of Fractions of Ethyl Acetate Extract of Garcinia Lattissima Miq. Fruits

The Antibacterial Activity of Fractions of Ethyl Acetate Garcinia Latissima Miq. Stem Bark Extracts Against Bacillus Subtilis and Pseudomonas Aeruginosa

Chemo‐taxonomic and biological potential of highly therapeutic plant Pedicularis groenlandica Retz. using multiple microscopic techniques

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