Antibacterial Activity of Fractions of Ethyl Acetate Extract of Garcinia Lattissima Miq. Fruits

Abstract

Objectives: The emergence of new infections and increase in bacterial drug resistance has created a serious need for the expansion of new antibacterial agents from natural sources. The study was carried out to evaluate the antibacterial activity of fractions of ethyl acetate extract of Garcinia latissima Miq. fruits. Methods:The fractionation was done using a silica gel column and organic solvents as the eluent, i.e., n-hexane, ethyl acetate, and methanol. All fractions were assayed for antibacterial activity, which was done by performing disc diffusion for growth inhibition against Bacillus subtilis and Pseudomonas aeruginosa. In addition, the growth inhibition activity was also examined by performing bioautography assay using pre-coated silica gel 60 GF 254 plates as the stationary phase. Fractions A-F were eluted using n-hexane:chloroform (1:4), while Fractions G-K were used ethyl acetate:dichloromethane (4:1) as the mobile phase. The plate was visualized by ultraviolet at λ 254 nm and 366 nm, while the other one was contacted with the inoculated agar medium to observe zone inhibition. Further, the minimum inhibitory concentration (MIC) value was determined by performing microdilution. Results:The result showed that the antibacterial activity of all fractions was more active at inhibiting the growth of B. subtilis than P. aeruginosa, mainly for Fractions H and J. However, the strongest antibacterial activity was showed by Fractions H and J against B. subtilis, MIC 312.5 µg/mL (lower than reference, which is erythromycin antibiotic (25 µg/mL), followed by Fraction D against B. subtilis MIC 625 µg/mL, Fraction K against P. aeruginosa MIC 625 µg/mL, whereas Fractions C, E, and G against B. subtilis, and Fraction E against P. aeruginosa also showed low MIC values (1.250 µg/mL). Conclusions:The results indicated that fractions of G. latissima Miq. fruit ethyl acetate extracts possessed antibacterial activity. The most active fraction that inhibited the growth of B. subtilis was shown by Fractions H and J; these fractions have the potential to be developed as new antibacterial agents.

Isolation and Antibacterial Activity by <i>in vitro</i> and <i>in silico</i> Approach of 6-Deoxyjacareubin Compound from <i>Garcinia latissima</i> Miq. Fruit

“…Fraction C had the highest activity from 11 fractions (FA-K). The result of the activity test against B. subtilis from the previous study of the fraction C 2% in DMSO provided inhibition zone diameter of 9.46 mm and MIC of 1250 ppm [7].…”

“…Moreover, the G. latissima fruits ethyl acetate extract (157 g) was fractionated. The fractionation was conducted using column chromatography according to the study by Ambarwati et al [7] and resulted in 11 fractions (A-K).…”

Antibacterial Activity Against Bacillus Subtilis and Antioxidant Properties of Methanol Extracts From Garcinia Latissima Miq. Leaves

“…The relationship between antibacterial activity and contents of active compounds in plant extracts or fractions of G. latissima have been investigated in multiple previous studies [1,[17][18][19][20][21]. Moreover, phytochemical tests of methanol extracts of G. latissima leaves showed the presence of tannin and saponin, which are known phytochemicals with health benefits and biological activities [4].…”