Antibacterial Activity Against Bacillus Subtilis and Antioxidant Properties of Methanol Extracts From Garcinia Latissima Miq. Leaves

Ambarwati, Neneng Siti Silfi; Elya, Berna; Malik, Amarila; Hanafi, Muhammad; Omar, Hasmahzaiti

doi:10.22159/ijap.2018.v10s1.06

Cited by 3 publications

(10 citation statements)

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“…The material used for the isolation was the combination of fractions B and C (0.51 g), and the combination of fractions D and E (1.84 g) of methanolic extract of G. latissima Miq. leaves [6]. The fractions B and C have a minimum inhibitory concentration (MIC) of 312.5 ppm and the fractions C and D have MIC 625 ppm [6].…”

Section: Isolation Processes Methods and Isolate Identification Methodsmentioning

confidence: 99%

“…leaves [6]. The fractions B and C have a minimum inhibitory concentration (MIC) of 312.5 ppm and the fractions C and D have MIC 625 ppm [6]. First of all, the combination of fraction B and fraction C and the combination of fraction D and fraction E was identified to determine the chromatogram pattern using thin-layer chromatography (TLC).…”

Section: Isolation Processes Methods and Isolate Identification Methodsmentioning

confidence: 99%

“…: B-949/IPH.3/KS/III/2017). Extracts (by maceration successively method) and fractions (by open column chromatography method) were obtained from previous studies in Laboratory Pharmacognosy-Phytochemistry at the Faculty of Pharmacy, Universitas Indonesia [6,9]. The process of fractionation using silica gel as the stationary phase and mobile phases used nhexane, ethyl acetate, and methanol, in order of increasing polarity [6].…”

Section: Plant Material Extracts and Fractionsmentioning

confidence: 99%

“…The results of the fractionation of the methanol extract of the leaves of G. latissima Miq. yielded 14 fractions (fraction A to fraction N) and fraction B and fraction C had the highest activity with MIC 312.5 µg/ml [6].…”

Section: Introductionmentioning

confidence: 99%

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Isolation, Identification, and Antibacterial of Amentoflavone From Garcinia Latissima Miq. Leaves

et al. 2022

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Objective: This study aimed to isolate and identify the Bacillus subtilis antibacterial compound present in the leaves of the Garcinia latissima Miq. Methods: The extracts were obtained by maceration successively, and the active extract was fractionated by column chromatography. The isolation of the most active fraction was performed by open column chromatography and preparative thin-layer chromatography. The isolate antibacterial assay against Bacillus subtilis by microdilution method. The compound structure of the isolate was identified by the nuclear magnetic resonance method. Results: The most active extract of the extracts from Garcinia latissima Miq. leaves against Bacillus subtilis are the methanol extract. The most active fractions of the fractions from G. latissima Miq. Leaves methanolic extract was isolated. The active isolate was identified as Amentoflavone. Conclusion: G. latissima Miq. leaves had the potential to be used as antibacterial medicinal herbs and one of the isolates of G. latissima Miq. is amentoflavone (active against B. Subtilis).

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Section: Isolation Processes Methods and Isolate Identification Methodsmentioning

confidence: 99%

Section: Isolation Processes Methods and Isolate Identification Methodsmentioning

confidence: 99%

Section: Plant Material Extracts and Fractionsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Isolation, Identification, and Antibacterial of Amentoflavone From Garcinia Latissima Miq. Leaves

et al. 2022

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“…The IC 50 value for oil and standard was 134. 23 [31]. Many reports indicated that hexadecanoic acid and oleic acid active antimicrobial, cytotoxic, and antidiarrheal agent activities of different medicinal plants [32,33].…”

Section: In Vitro Antioxidant Assaymentioning

confidence: 99%

Chemical Profiling of Buchanania Lanzan Spreng Essential Oil and Its Biological Activities

Ajith

Krishna

et al. 2020

Asian J Pharm Clin Res

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Objective: The present study was designed to evaluate the chemical composition of the essential oil of Buchanania lanzan Spreng extracted from the seeds and to evaluate in vitro antimicrobial antioxidants and molecular docking studies of the major bioactive compounds of essential oil. Methods: The essential oil was obtained by hydrodistillation of the B. lanzan seeds and analyzed by gas chromatography-mass spectrometry (GC-MS). Antibacterial activity was evaluated against Pseudomonas aeruginosa, Salmonella typhi, Vibrio cholerae, Staphylococcus aureus, and Streptococcus pneumoniae clinical isolates by disk diffusion method and resazurin assay determined the minimum inhibitory concentration. The in vitro antioxidant activity was determined by 2,2-diphenyl-1-picrylhydrazyl (DPPH) and hydrogen peroxide (H2O2) scavenging assay; the essential oil major bioactive compounds are Androstan-3-ol, Campesterol, and γ-Sitosterol were docked against bacterial protein DNA gyrase. Results: GC-MS analysis exhibited the presence of 19 bioactive compounds. The essential oil showed that significant antibacterial activity was noticed against V. cholerae and S. typhi with the highest zone of inhibition 15.67–1.20 and 13.83–0.33, respectively. Antioxidant activity in DPPH and H2O2 scavenging assays with IC50 values of 134.23 and 191.24, respectively. The molecular docking of Androstan-3-ol and γ-Sitosterol with bacterial DNA gyrase unveiled a good binding affinity of −6.4 kcal/mol and −6.3 kcal/ mol, respectively. Conclusion: It could be concluded that the essential oils potential sources of antibacterial, antioxidant activities, and molecular docking of bioactive components. The results of this study provide partial scientific support for the traditional application of essential oils to cure diarrhea and also major bioactive compounds responsible for important biological activities.

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Anti-elastase activity of methanolic and ethyl acetate extract from Garcinia latissima Miq.

Ambarwati

Elya

Desmiaty

2019

J. Phys.: Conf. Ser.

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Elastase is a proteinase enzyme that can reduce elastin by dividing specific peptide bonds. Therefore, the inhibition of elastase activity in the dermis layer can be used to maintain skin elasticity. Materials that can inhibit elastase activity can be a cosmetic ingredient in dealing with skin aging. Increased desire to maintain healthy skin without chemicals, encourages the use of materials from natural sources such as plants from Indonesia, a country that has high biodiversity, including Garcinia latissima Miq. from the Clusiaceae family. The purpose of this study was to explore and sought an explanation as to why G. latissima Miq. inhibits elastase enzyme. Plant extraction was carried out using maceration successively methods. The inhibition activity of elastase enzyme was carried out by measuring the kinetic enzyme N-succ-(Ala)-3-Nitroanilide conversion to p-nitroaniline (substrate) spectrophotometry at 405 nm and using porcine pancreatic elastase (PPE) as an enzyme. The results showed that the G. latissima Miq. methanol extract and ethyl acetate extract were active as elastase enzyme inhibitors. G. latissima Miq. extract can maintain skin elasticity.

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Antibacterial Activity Against Bacillus Subtilis and Antioxidant Properties of Methanol Extracts From Garcinia Latissima Miq. Leaves

Cited by 3 publications

References 20 publications

Isolation, Identification, and Antibacterial of Amentoflavone From Garcinia Latissima Miq. Leaves

Isolation, Identification, and Antibacterial of Amentoflavone From Garcinia Latissima Miq. Leaves

Chemical Profiling of Buchanania Lanzan Spreng Essential Oil and Its Biological Activities

Anti-elastase activity of methanolic and ethyl acetate extract from Garcinia latissima Miq.

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