Antimicrobial Activity and Identification The Compounds of Methanol Extract from The Pleurotus Ostreatus Fruiting Body

Prastiyanto, Muhammad Evy; darmwati, Sri; Iswara, Arya; Setyaningtyas, Agnis; Trisnawati, Laily; Syafira, Anna

doi:10.18860/elha.v6i1.4082

Cited by 11 publications

(7 citation statements)

References 6 publications

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“…So, novel antibacterial agents are needed from natural biological sources (Valle Jr et al, 2015). Biological antibacterial agents can be obtained from the mushrooms (Prastiyanto et al, 2016), bacteria (Ryandini et al, 2018), bacteriocins (Lestari et al, 2019), and plant (Tillah et al, 2017;Wahyuni et al, 2019). There have been many reports found in the medical literature concerning the significance of traditional medicinal plants as alternatives to antibacterial agents (Akhtar, 2015;Aumeeruddy-elalfi et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

Antibacterial Activity of Various Extracts of Averrhoa bilimbi against Multidrug Resistant Bacteria

et al. 2020

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The multi-drug resistance (MDR) bacteria is a global health problem that causes high mortality every year. Therefore, novel antibacterial agents are needed from natural biological sources. This research aimed to investigate the antibacterial activities of various crude extracts of Averrhoa bilimbi against MDR bacteria. The antibacterial activity was calculated based on the use agar well diffusion assay and the minimum bactericidal concentration (MBC) using Mueller–Hinton broth in a microdilution method. Bacteria from wells were subcultured using inoculating loop onto a 5% sheep BAP. The best antibacterial activity, calculated as the most widely inhibitory zone and the smallest MBC values. The ethanolic extract showed antibacterial activity against the all MDR bacterial test in the agar well diffusion assay (10-14.5 mm inhibition diameter). The MBC of water extract against ESβL + CR Pseudomonas aeruginosa showed the best antibacterial activity (12.5 mg/mL). The fruit of bilimbi was shown to be potentially developed as antibacterial agents, especially for MDR strains. Further in vivo research and discovery of action mode are needed to shed light on their antibacterial effects. This study can provide new information about the benefits of bilimbi as a source of natural antibacterial againts MDR-bacteria

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Section: Introductionmentioning

confidence: 99%

Antibacterial Activity of Various Extracts of Averrhoa bilimbi against Multidrug Resistant Bacteria

et al. 2020

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“…Natural anti-MDR bacterial agents can be obtained from fruits (Prastiyanto et al 2020d), seeds (Prastiyanto et al 2020a;Prastiyanto 2021), Latex (Prastiyanto et al 2020c) lactic acid bacteria (Lestari et al 2019), mushroom (Prastiyanto et al 2020b(Prastiyanto et al , 2016, and bacterial isolates from marine organisms (Al-dhabi et al 2020) . In recent years, studies of marine bioactive compounds have yielded many drug candidates (Webster and Taylor 2012).…”

Section: Esβl-producingmentioning

confidence: 99%

Bioprospecting of bacterial symbionts of sponge Spongia officinalis from Savu Sea, Indonesia for antibacterial potential against multidrug-resistant bacteria

et al. 2022

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Abstract. Prastiyanto ME, Kartika AI, Darmawati S, Radjasa OK. 2022. Bioprospecting of bacterial symbionts of sponge Spongia officinalis from Savu Sea, East Nusa Tenggara, Indonesia for antibacterial potential against multidrug-resistant bacteria. Biodiversitas 23: 1118-1124. Marine sponge Spongia sp. has been reported to have potential as an antibacterial agent. However, less information on the potential of bacterial symbionts of Spongia sp. as an antibacterial agent has been documented. The present investigation involves isolating bacterial symbionts of sponge Spongia officinalis and the characterization of antibacterial potential against multidrug-resistant bacteria isolated from clinical specimens. Spongia officinalis was collected from Savu Sea, East Nusa Tenggara, Indonesia and its symbionts were isolated with Zobell marine agar media. The overlay method was used to screen the antibacterial activity against selected six MDR bacteria. Antibacterial activity was determined by measuring the diameter of the inhibition zone. Identification of active bacterial symbionts was carried out based on the 16S rRNA gene sequencing. The results revealed that four out of 10 symbionts showed antibacterial activity against MDR bacteria with an inhibition index ranging from 4.8 to 12.6 mm. Prastiyanto-1A and Prastiyanto-1E isolates demonstrated antibacterial activity against ESBL- Escherichia coli and ESBL + CRE- Klebsiella pneumoniae subsp. pneumoniae, Pratiyanto-2A isolate showed antibacterial activity against MRSA, while Prastiyanto-4A isolates showed antibacterial activity against CRPA. The selected four isolates were identified as Bacillus subtilis, Bacillus mojavensis and Bacillus simplex using 16S rRNA gene sequencing and BLASTn analysis. These results provide information about the potential of bacterial symbionts of S. officinalis as natural antibacterial sources against MDR bacteria.

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“…Antibacterial agents derived from biological sources include mushrooms (Prastiyanto et al 2016;2020b), lactic acid bacteria (Lestari et al 2019), latex (Prastiyanto et al 2020c), fruits (Prastiyanto et al 2020d), and seeds (Prastiyanto et al 2020a). Many studies on antimicrobial agents report the importance of plants as alternative antimicrobial agents (Prastiyanto et al 2021a;Prastiyanto et al 2021b).…”

Section: Introductionmentioning

confidence: 99%

Seeds extract of three Artocarpus species: Their in-vitro antibacterial activities against multidrug-resistant (MDR) Escherichia coli isolates from urinary tract infections (UTIs)

Prastiyanto

2021

Biodiversitas

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Abstract. Prastiyanto ME. 2021. Seeds extract of three Artocarpus species: Their in-vitro antibacterial activities against multidrug-resistant (MDR) Escherichia coli isolates from urinary tract infections (UTIs). Biodiversitas 22: 4362-4368. Multidrug-resistant (MDR)-E. coli is a major cause and has become a very serious problem in urinary tract infections (UTIs). As a result, it requires an antibacterial agent derived from biological materials. It has been reported that the seeds of three species of Artocarpus (A. heterophyllous, A. champeden, and A. camansi) have antibacterial properties against Methicillin-Resistant Staphylococcus aureus (MRSA). However, there are three other Artocarpus species in Indonesia, i.e., keledang (A. lanceipolius), tarra (A. elasticus), and terap (A. Odoratissimus) whose antibacterial property has not been investigated. To minimize the research gap, this study aims to determine the antibacterial activity of seed extracts of A. lanceipolius, A. elasticus, and A. odoratissimus against MDR-E. coli isolates of UTIs. Antibacterial activity was evaluated using the agar well diffusion assay. The microdilution method was used to determine the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) values. The results revealed that the seed extracts of A. lanceipolius, A. elasticus, and A. odoratissimus have the potential as antibacterial agents against MDR-E. coli isolate of UTIs. A. elasticus seed extract shows the widest zone of inhibition in the range of 7.0-13.3 mm and the smallest MIC and MBC values ??of 6.25-12.5 mg/mL and 12.5-25 mg/mL, respectively. In conclusion, A. lanceipolius, A. elasticus, and A. odoratissimus seed extracts have the potential to be developed as antibacterial agents against UTI-causing MDR-E. coli. Further in vivo research and determining the mode of action of antibacterial activity are needed.

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Antimicrobial Activity and Identification The Compounds of Methanol Extract from The Pleurotus Ostreatus Fruiting Body

Cited by 11 publications

References 6 publications

Antibacterial Activity of Various Extracts of Averrhoa bilimbi against Multidrug Resistant Bacteria

Antibacterial Activity of Various Extracts of Averrhoa bilimbi against Multidrug Resistant Bacteria

Bioprospecting of bacterial symbionts of sponge Spongia officinalis from Savu Sea, Indonesia for antibacterial potential against multidrug-resistant bacteria

Seeds extract of three Artocarpus species: Their in-vitro antibacterial activities against multidrug-resistant (MDR) Escherichia coli isolates from urinary tract infections (UTIs)

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