Antigenicity to neuropeptide F (NPF) in Stenostomum leucops and Microstomum lineare

Grahn, Margareta; Maule, Aaron G.; Elo, Iina; Shaw, Christopher; Reuter, Maria; Halton, D W

doi:10.1007/978-94-011-0045-8_59

Cited by 3 publications

(3 citation statements)

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“…To date, there are few data available on PEG-m-THPC-mediated PDT effects. Grahn (39) found that 6 mgkg PEG-m-THPC induced a 6 mm-deep necrosis in tumors of a colon carcinoma in a mouse model 10 days following drug application. Simultaneously, very little muscle swelling has been observed when a leg was exposed to PDT.…”

Section: Discussionmentioning

confidence: 99%

“…However, preparation of this photosensitizer in aqueous solution for systemic application is difficult due to its hydrophobicity. The addition of four long hydroxylpolyethylene glycol (PEG 2000) side chains linked to m-THPC through a triazine group produces a tetrakis-(mmethoxypolyethylene glycol) derivative of 7,8-dihydro-5,10,15,20-tetrakis(3-hydroxyphenyl)-2 1,23-[HI-porphyrin (PEG-m-THPC), with high hydrophilicity and no instability during in v i m experiments (39). The molecular weight of this water-soluble compound is 65 15 Da, compared to 680 for the hydrophobic parent sensitizer, m-THPC.…”

Section: Introductionmentioning

confidence: 99%

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Highly Selective Targeting of Ovarian Cancer with the Photosensitizer PEG-m-THPC in a Rat Model

et al. 1999

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Photodynamic therapy (PDT) uses light to activate a photosensitizer that has been absorbed or retained preferentially by cancer cells after systemic administration. The first pegylated photosensitizer, tetrakis-(m-methoxypolyethylene glycol) derivative of 7,8-dihydro-5,10,15,20-tetrakis(3-hydroxyphenyl)-21,23-[H]-porphyrin (PEG-m-THPC), was evaluated to target selectively unresectable pelvic ovarian cancer bulks. Our goals were two-fold: (1) to establish an ovarian cancer model suitable for the development of debulking techniques and (2) to characterize the pharmacokinetics and tumor selectivity of PEG-m-THPC by fluorescence microscopy. NuTu-19 ovarian cancer cells were injected into the caudal part of the right psoas muscle of Fisher rats. Five weeks later, 30 mg/kg body weight of PEG-m-THPC was injected intravenously. Necropsy was performed between 4 and 10 days following drug application, and fluorescence of the tumor and various abdominal organs was measured. All rats developed bulky pelvic tumors with an average diameter of 2.6 cm (+/- 0.6 SD). Tumor masses were encompassing and infiltrating pelvic organs in a similar manner to ovarian cancers in humans. Fluorescence of cancer tissue was maximal 8-10 days following drug application. At 8 days, the tumor-to-tissue ratio was 40:1 (+/- 12 SE) for most abdominal organs. We conclude that this tumor model may be used for the study of new pelvic debulking techniques, and that the tumor selectivity of PEG-m-THPC is exceptionally high 8 days after drug application. Based on these data, we are currently developing a PDT-based minimally invasive debulking technique for advanced ovarian cancer.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Highly Selective Targeting of Ovarian Cancer with the Photosensitizer PEG-m-THPC in a Rat Model

et al. 1999

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“…For example, it can be easily maintained and reproduces quickly in culture, regenerates extensively and has internal organs that can be readily observed (Nuttycombe & Waters, 1938). For more than a century, Stenostomum, primarily S. leucops, have been used for several experimental biology studies, such as studies of fission and regeneration (Ritter & Congdon, 1900;Child, 1990Child, , 1902Hartmann, 1922;Ruhl, 1927;Van Cleave, 1929), stem cells (Palmberg, 1990), ultrastructure of sensory organs (Reuter et al, 1993;Palmberg & Reuter, 1992;Ruppert & Schreiner 1980), ultrastructure of the digestive tract (Antoniazzi & Silveira, 1996), senescence (Martínez & Levinton, 1992), neuropeptides (Grahn et al, 1995;Wikgren & Reuter, 1985) and ecology (Nandini & Sarma, 2013;Nandini et al, 2011). However, some basic aspects of the biology of these worms, such as the time required for paratomy or the number of cells constituting the body, are not well known.…”

Section: Introductionmentioning

confidence: 99%

Stenostomum Leucops Dugès, 1828 (Platyhelminthes, Catenulida): A Putative Species Complex With Phenotypic Plasticity

Rosa¹,

Pereira²,

Ragagnin³

et al. 2015

Pap. Avulsos Zool. (São Paulo)

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The organization of the nervous system in Plathelminthes. The neuropeptide F-immunoreactive pattern in Catenulida, Macrostomida, Proseriata

et al. 1995

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Antigenicity to neuropeptide F (NPF) in Stenostomum leucops and Microstomum lineare

Cited by 3 publications

References 1 publication

Highly Selective Targeting of Ovarian Cancer with the Photosensitizer PEG-m-THPC in a Rat Model

Highly Selective Targeting of Ovarian Cancer with the Photosensitizer PEG-m-THPC in a Rat Model

Stenostomum Leucops Dugès, 1828 (Platyhelminthes, Catenulida): A Putative Species Complex With Phenotypic Plasticity

The organization of the nervous system in Plathelminthes. The neuropeptide F-immunoreactive pattern in Catenulida, Macrostomida, Proseriata

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