Stenostomum Leucops Dugès, 1828 (Platyhelminthes, Catenulida): A Putative Species Complex With Phenotypic Plasticity

Rosa, Marcos Trindade; Pereira, Conceição; Ragagnin, Geovani Tolfo; Loreto, Élgion Lúcio da Silva

doi:10.1590/0031-1049.2015.55.27

Cited by 6 publications

(8 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This strain was characterized by Rosa et al. (). Vouchers of the strains used were deposited in the UFSM Department of Biology collection under numbers L01‐sm01 and SL01‐sm01.…”

Section: Methodsmentioning

confidence: 99%

“…In this study, S. leucops strain SL01-sm01 was used and maintained in laboratory cultures for more than 6 years. This strain was characterized by Rosa et al (2015). Vouchers of the strains used were deposited in the UFSM Department of Biology collection under numbers L01-sm01 and SL01-sm01.…”

Section: Biological Samples and Molecular Genetic Proceduresmentioning

confidence: 99%

“…The time spent in paratomy is approximately 42.5 h at 28°C, producing zooids containing approximately 2400 cells. Two zooids commonly form; however, it is a plastic phenotype capable of producing up to five zooids and subsequently new worms depending on the environmental conditions (Rosa et al 2015). Phylogenetic studies, using different genes, suggest that this taxon corresponds to a species complex Rosa et al 2015).…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Characterization of the first mitochondrial genome of a catenulid flatworm:Stenostomum leucops(Platyhelminthes)

Rosa

Oliveira²,

Loreto

2017

J Zool Syst Evol Res

View full text Add to dashboard Cite

The first complete mitochondrial genome of a catenulid, Stenostomum leucops, was characterized. Illumina sequencing and 90 813 reads were utilized in the assembly, producing one contig with an average coverage of 1118×. The length of this genome is 15 742 bp with 12 protein‐coding, two rRNA and 22 tRNA genes. Although the atp8 gene is absent in other Platyhelminthes, a highly divergent putative atp8 gene was found in S. leucops. In contrast to other Platyhelminthes, the mitochondrial genes of S. leucops are encoded on both strands. The gene order in the S. leucops mitogenome is very divergent from those observed in other Platyhelminthes, showing only small blocks of synteny. With AAA as the codon for lysine S. leucops shows the probable plesiomorphic condition, whereas Rhabditophora possess the derived GAA. This evolutionary transition is correlated with changes in the respective anticodons in trnK. It remains unclear whether the absence of the D arm and loop in trnS1 is a convergence in Catenulida and Neodermata.

show abstract

“…This strain was characterized by Rosa et al. (). Vouchers of the strains used were deposited in the UFSM Department of Biology collection under numbers L01‐sm01 and SL01‐sm01.…”

Section: Methodsmentioning

confidence: 99%

Section: Biological Samples and Molecular Genetic Proceduresmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Characterization of the first mitochondrial genome of a catenulid flatworm:Stenostomum leucops(Platyhelminthes)

Rosa

Oliveira²,

Loreto

2017

J Zool Syst Evol Res

View full text Add to dashboard Cite

show abstract

“…As these materials can be provided by the microbiome, this provides a new route for holobionts to increase their arsenal of evolutionary tools. Catenulida show extraordinary phenotypic plasticity 13 . Also, their microbiome, as shown here, can be highly variable.…”

Section: Discussionmentioning

confidence: 99%

“…In S. leucops , the paratomy process occurs in approximately 42 h at 28 °C, producing zooids containing approximately 2400 cells. These flatworms range from 0.5 to 2 mm in length 13 . The first requisite of genetic transformation, the incorporation of foreign DNA into a cell, is the entrance of DNA into the cell.…”

Section: Introductionmentioning

confidence: 99%

The Catenulida flatworm can express genes from its microbiome or from the DNA it ingests

Rosa

Loreto

2019

Sci Rep

View full text Add to dashboard Cite

Stenostomum are tiny planarians of the phylum Platyhelminthes that reproduce asexually. We transfected these worms using plasmids containing a gfp reporter gene. Here we show that they can express genes present in plasmids carried by bacteria and those that are encoded by naked DNA, such as plasmids or PCR fragments, transfected by electroporation; they can also express genes taken up during feeding. The microbiome associated with worm maintenance was evaluated, and the results indicated that when a plasmid is maintained in the microbiome, gfp gene expression is stable. When genes originate from naked DNA or bacteria not maintained in the microbiome, GFP expression is transient. Therefore, changes in the microbiome can modify the ability of worms to express foreign genes. In stable GFP-expressing worms, NSG showed that the gfp gene was maintained in the plasmid and was not integrated into the chromosome. These results suggest that, at least for some organisms such as flatworms, the expression of genes provided by the microbiome or the environment can be considered among the potential sources of phenotypic plasticity, which can have implications for evolvability.

show abstract

Stenostomum leucops (Catenulida, Platyhelminthes) has a flexible microbiome in time and space

Rosa

Loreto²

2022

Hydrobiologia

View full text Add to dashboard Cite

Stenostomum Leucops Dugès, 1828 (Platyhelminthes, Catenulida): A Putative Species Complex With Phenotypic Plasticity

Abstract: Species of

Cited by 6 publications

References 35 publications

Characterization of the first mitochondrial genome of a catenulid flatworm:Stenostomum leucops(Platyhelminthes)

Characterization of the first mitochondrial genome of a catenulid flatworm:Stenostomum leucops(Platyhelminthes)

The Catenulida flatworm can express genes from its microbiome or from the DNA it ingests

Stenostomum leucops (Catenulida, Platyhelminthes) has a flexible microbiome in time and space

Contact Info

Product

Resources

About