Antifungal cellobiose lipid secreted by the epiphytic yeast Pseudozyma graminicola

Golubev, W. I.; Kulakovskaya, T. V.; Шашков, А. С.; Kulakovskaya, Ekaterina; Golubev, N. V.

doi:10.1134/s0026261708020082

Cited by 20 publications

(17 citation statements)

References 8 publications

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“…Pseudozyma fusiformata, P. graminicola, and P. flocculosa were also known as CL producers (Kulakovskaya et al, 2005;Mimee et al, 2005;Golubev et al, 2008). Pseudozyma fusiformata, P. graminicola, and P. flocculosa were also known as CL producers (Kulakovskaya et al, 2005;Mimee et al, 2005;Golubev et al, 2008).…”

Section: Resultsmentioning

confidence: 99%

“…Ustilago maydis has been known to be the most traditional producer for CLs (as the ustilagic acids) together with MELs (as the ustilipids). Pseudozyma fusiformata, P. graminicola, and P. flocculosa were also known as CL producers (Kulakovskaya et al, 2005;Mimee et al, 2005;Golubev et al, 2008). In U. maydis and P. flocculosa, the gene cluster is expressed under the nitrogen-limited conditions (Teichmann et al, 2007(Teichmann et al, , 2011.…”

Section: Resultsmentioning

confidence: 99%

“…They have been receiving much attention because of their strong fungicidal activity on a variety of fungi, including pathogenic species (Kulakovskaya et al, 2005). The other CL producers are Pseudozyma fusiformata and P. graminicola (Kulakovskaya et al, 2005;Golubev et al, 2008), and P. flocculosa (Mimee et al, 2005(Mimee et al, , 2009. The other CL producers are Pseudozyma fusiformata and P. graminicola (Kulakovskaya et al, 2005;Golubev et al, 2008), and P. flocculosa (Mimee et al, 2005(Mimee et al, , 2009.…”

Section: Introductionmentioning

confidence: 99%

“…Several fungal strains have been reported to produce different types of CLs, and the most representative producer is a smut fungus, Ustilago maydis, whose main product is ustilagic acid under nitrogen-limited conditions (Spoeckner et al, 1999). The other CL producers are Pseudozyma fusiformata and P. graminicola (Kulakovskaya et al, 2005;Golubev et al, 2008), and P. flocculosa (Mimee et al, 2005(Mimee et al, , 2009.…”

Section: Introductionmentioning

confidence: 99%

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Accumulation of cellobiose lipids under nitrogen-limiting conditions by two ustilaginomycetous yeasts,Pseudozyma aphidisandPseudozyma hubeiensis

et al. 2013

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Some basidiomycetous yeast strains extracellularly produce cellobiose lipids (CLs), glycolipid biosurfactants which have strong fungicidal activity. The representative CL producer Ustilago maydis produces CLs together with the other glycolipids, mannosylerythritol lipids (MELs); the preference of the two glycolipids is affected considerably by the nitrogen source. To develop new CL producers, 12 MEL producers were cultured under the nitrogen-limited conditions. Pseudozyma aphidis and Pseudozyma. hubeiensis were characterized as new CL producers. CL production was induced on three strains, P. aphidis, Pseudozyma graminicola, and P. hubeiensis under these conditions. The putative homologous genes of U. maydis cyp1, which encodes a P450 monooxygenase, essential for CL biosynthesis, were partially amplified from their genomic DNA. The nucleotide sequences of the gene fragments from P. hubeiensis and P. aphidis shared identities with U. maydis cyp1 of 99% and 78%, respectively. Furthermore, all of the deduced translation products are tightly clustered in the phylogenic tree of the monooxygenase. These results suggest that the genes involved with CL biosynthesis must be widely distributed in the basidiomycetous fungi as well as the MEL biosynthesis genes, and thus, the genus Pseudozyma has great potential as a biosurfactant producer.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Accumulation of cellobiose lipids under nitrogen-limiting conditions by two ustilaginomycetous yeasts,Pseudozyma aphidisandPseudozyma hubeiensis

et al. 2013

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“…1b and c) was chosen. These molecules are naturally produced by epiphytic fungi (Golubev et al, 2008;Kulakovskaya et al, 2004Kulakovskaya et al, , 2005, of which Ustilago maydis is the most important example, and by a limited number of non-ustilageneous yeast species (Kulakovskaya et al, 2007(Kulakovskaya et al, , 2010. They gain interest because of their antifungal action (Kulakovskaya et al, 2009;Mimee et al, 2005) and synergistic effects with human antifungal therapeutics (Mimee et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

Candida bombicola as a platform organism for the production of tailor‐made biomolecules

Roelants

Saerens

Derycke

et al. 2013

Biotech & Bioengineering

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The yeast Candida bombicola is capable of producing high amounts (400 g/L) of the biosurfactant sophorolipids. The genetic makeup of this industrially important yeast has recently been uncovered and molecular manipulation techniques have been developed. Hence, all tools for the development of new bioprocesses with C. bombicola are now available. As a proof of concept, the production of two totally different molecules was aimed for: the bioplastic polyhydroxyalkanoate (PHA) and a new-to-nature cellobioselipid-biosurfactant. Integration of the new functionalities at genomic loci necessary for sophorolipid production safeguards the new biomolecules from sophorolipid contamination, while taking advantage of the regulation of the sophorolipid gene cluster. A maximum yield of 2.0% wt/dwt PHA was obtained; furthermore, this is the first time cellobioselipid synthesis by a non-natural producer is reported. We here provided proof of concept that C. bombicola can be transformed into a platform organism for the production of tailor-made biomolecules.

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Yeast glycolipid biosurfactants

2017

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Edited by Wilhelm JustVarious yeasts, both conventional and exotic ones, are known to produce compounds useful to mankind. Ethanol is the most known of these compounds, but more complex molecules such as amphiphilic biosurfactants can also be derived from eukaryotic microorganisms at an industrially and commercially relevant scale. Among them, glycolipids are the most promising, due to their attractive properties and high product titers. Many of these compounds can be considered as secondary metabolites with a specific function for the host. Hence, a dedicated biosynthetic process enables regulation and combines pathways delivering the lipidic moiety and the hydrophilic carbohydrate part of the glycolipid. In this Review, we will discuss the biosynthetic and regulatory aspects of the yeast-derived sophorolipids, mannosylerythritol lipids, and cellobiose lipids, with special emphasis on the relation between glycolipid synthesis and the general lipid metabolism.

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Antifungal cellobiose lipid secreted by the epiphytic yeast Pseudozyma graminicola

Cited by 20 publications

References 8 publications

Accumulation of cellobiose lipids under nitrogen-limiting conditions by two ustilaginomycetous yeasts,Pseudozyma aphidisandPseudozyma hubeiensis

Accumulation of cellobiose lipids under nitrogen-limiting conditions by two ustilaginomycetous yeasts,Pseudozyma aphidisandPseudozyma hubeiensis

Candida bombicola as a platform organism for the production of tailor‐made biomolecules

Yeast glycolipid biosurfactants

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