<i>Candida bombicola</i> as a platform organism for the production of tailor‐made biomolecules

Roelants, Sophie; Saerens, Karen; Derycke, Thibaut; Li, Bing; Lin, Yao‐Cheng; Peer, Yves Van de; Maeseneire, Sofie L. De; Bogaert, Inge Van; Soetaert, Wim

doi:10.1002/bit.24895

Cited by 42 publications

(24 citation statements)

References 50 publications

(65 reference statements)

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“…glucolipids, acidic sophorolipids, cellobiose lipids, etc. (Ciesielska et al 2014;Roelants et al, 2013;Saerens et al, 2011a)). In this manuscript, we describe how our pursuit to produce non-acetylated acidic sophorolipids, led to a strain…”

Section: Acc E P Ted P R E P R I Ntmentioning

confidence: 92%

Synthesis of bolaform biosurfactants by an engineered Starmerella bombicola yeast

Bogaert

Buyst

Martins

et al. 2016

Biotech & Bioengineering

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Bola-amphiphilic surfactants are molecules with fascinating properties. Their unique configuration consisting of a long hydrophobic spacer connecting two hydrophilic entities renders the molecule more water soluble than the average surfactant, but still allows formation of supramolecular structures. These properties make them extremely suitable for applications in in nanotechnology, electronics, and gene and drug delivery. In general, these compounds are obtained by chemical synthesis. We report here an efficient microbial production process for the fully green synthesis of bolaform surfactants. A sophorolipid-producing Starmerella bombicola yeast strain was disabled in its sophorolipid acetyltransferase and lactone esterase, which should logically result in synthesis of non-acetylated acidic sophorolipids; molecules with the classic amphiphilic structure. Surprisingly, also bolaform glycolipids were obtained, with an additional sophorose linked to the free carboxyl end of the acidic sophorolipids as confirmed by MS and NMR analysis. The obtained titers of 27.7 g/L total product are comparable to wild type values, and the novel molecules account for at least 74% of this. Bola-amphiphile biosynthesis proved to be attributed to the promiscuous activity of both UDP-glucosyltransferases UGTA1 and UGTB1 from the core sophorolipid pathway, displaying activity toward non-acetylated intermediates. The absence of acetyl groups seems to trigger formation of bolaform compounds starting from acidic sophorolipids. Hence, wild type S. bombicola produces these compounds only at marginal amounts in general not reaching detection limits. We created a strain knocked-out in its sophorolipid acetyltransferase and lactone esterase able to produce these novel compounds in economical relevant amounts, opening doors for the application of biological-derived bolaform structures. Biotechnol. Bioeng. 2016;113: 2644-2651. © 2016 Wiley Periodicals, Inc.

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Section: Acc E P Ted P R E P R I Ntmentioning

confidence: 92%

Synthesis of bolaform biosurfactants by an engineered Starmerella bombicola yeast

Bogaert

Buyst

Martins

et al. 2016

Biotech & Bioengineering

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“…Candida bombicola is capable of producing large quantities of biosurfactant sophorolipids (Roelants et al ., ; Elshafie et al ., ; Konishi et al ., ; Samad et al ., ). Therefore, to further optimise the procedure, hydrolysed horse oil was fermented with C. bombicola in a 5 l jar fermenter.…”

Section: Resultsmentioning

confidence: 99%

A novel microbial technique for producing high‐quality sophorolipids from horse oil suitable for cosmetic applications

Maeng

Kim

Zhou

et al. 2018

Microbial Biotechnology

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SummaryHorse oil contains linoleic, palmitoleic and unsaturated fatty acids that are similar to those in human skin, and may therefore be an ideal substance from which to isolate biosurfactants for cosmetic products to improve human skin quality. Herein, an innovative approach was developed to synthesise sophorolipids from horse oil by hydrolysis, followed by fermentation using the yeast Candida bombicola. The yield of sophorolipids from direct fermentation of horse oil and hydrolysed horse oil was 40.6 ± 1.3 g l−1 and 58.4 ± 1.8 g l−1 respectively. To further increase the yield, 30–40 g l−1 glucose was added in a fed‐batch fermentation process to maintain the pH between 4.0 and 4.5, resulting in a conversion yield of 71.7 ± 0.8 g l−1. The purity and structure of the synthesised sophorolipids were analysed by ultra‐performance liquid chromatography‐mass spectrometry and nuclear magnetic resonance. An in vitro human dermal fibroblast model was used as a surrogate for human skin to measure elastase inhibition activity. Antiwrinkle properties of isolated sophorolipids were better than those of horse oil or hydrolysed horse oil in several in vitro assays. Furthermore, no cytotoxicity was observed at a concentration of 50 μg ml−1, and wound‐healing capacity was evident in a cell culture model. Additionally, the synthesised sophorolipids attenuated lipopolysaccharide‐induced expression of inflammatory cytokines in macrophages, and efficiently inhibited several strains of bacteria and yeast. In conclusion, fed‐batch fermentation of hydrolysed horse oil is a novel and efficient approach for producing high‐quality and high‐yield sophorolipids that exhibit great potential as cosmetic ingredients.

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“…In synthetic biology, to name but a few examples, single cell organisms are described as “genetically engineered machines” (iGEM Foundation n.d.), intracellular molecular processes are “genetic circuits” (Weiss et al 2003), and genetically engineered organisms are referred to as “platform organisms” (Roelants et al 2013). Obviously, synthetic biology makes use of the metaphor of the machine and its variants, most notably its information technology equivalent, the computer, as a central conceptual lens to describe, explain and modify molecular intra- and intercellular processes.…”

Section: The Machine Metaphor and Its Implicationsmentioning

confidence: 99%

Machine metaphors and ethics in synthetic biology

Boldt

2018

Life Sci Soc Policy

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The extent to which machine metaphors are used in synthetic biology is striking. These metaphors contain a specific perspective on organisms as well as on scientific and technological progress. Expressions such as “genetically engineered machine”, “genetic circuit”, and “platform organism”, taken from the realms of electronic engineering, car manufacturing, and information technology, highlight specific aspects of the functioning of living beings while at the same time hiding others, such as evolutionary change and interdependencies in ecosystems. Since these latter aspects are relevant for, for example, risk evaluation of uncontained uses of synthetic organisms, it is ethically imperative to resist the thrust of machine metaphors in this respect. In addition, from the perspective of the machine metaphor viewing an entity as a moral agent or patient becomes dubious. If one were to regard living beings, including humans, as machines, it becomes difficult to justify ascriptions of moral status. Finally, the machine metaphor reinforces beliefs in the potential of synthetic biology to play a decisive role in solving societal problems, and downplays the role of alternative technological, and social and political measures.

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Candida bombicola as a platform organism for the production of tailor‐made biomolecules

Cited by 42 publications

References 50 publications

Synthesis of bolaform biosurfactants by an engineered Starmerella bombicola yeast

Synthesis of bolaform biosurfactants by an engineered Starmerella bombicola yeast

A novel microbial technique for producing high‐quality sophorolipids from horse oil suitable for cosmetic applications

Machine metaphors and ethics in synthetic biology

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