Accumulation of cellobiose lipids under nitrogen-limiting conditions by two ustilaginomycetous yeasts,Pseudozyma aphidisandPseudozyma hubeiensis

Abstract

Some basidiomycetous yeast strains extracellularly produce cellobiose lipids (CLs), glycolipid biosurfactants which have strong fungicidal activity. The representative CL producer Ustilago maydis produces CLs together with the other glycolipids, mannosylerythritol lipids (MELs); the preference of the two glycolipids is affected considerably by the nitrogen source. To develop new CL producers, 12 MEL producers were cultured under the nitrogen-limited conditions. Pseudozyma aphidis and Pseudozyma. hubeiensis were characterized as new CL producers. CL production was induced on three strains, P. aphidis, Pseudozyma graminicola, and P. hubeiensis under these conditions. The putative homologous genes of U. maydis cyp1, which encodes a P450 monooxygenase, essential for CL biosynthesis, were partially amplified from their genomic DNA. The nucleotide sequences of the gene fragments from P. hubeiensis and P. aphidis shared identities with U. maydis cyp1 of 99% and 78%, respectively. Furthermore, all of the deduced translation products are tightly clustered in the phylogenic tree of the monooxygenase. These results suggest that the genes involved with CL biosynthesis must be widely distributed in the basidiomycetous fungi as well as the MEL biosynthesis genes, and thus, the genus Pseudozyma has great potential as a biosurfactant producer.

Biological control of the cucurbit powdery mildew pathogen Podosphaera xanthii by means of the epiphytic fungus Pseudozyma aphidis and parasitism as a mode of action

“…provide further support for antibiosis as they reveal the presence of gene clusters regulating synthesis of glycolipids, some of which have an antibiosis ability (Lefebvre et al, 2013; Morita et al, 2013a, 2014; Lorenz et al, 2014; Saika et al, 2014). Flocculosin, synthesized by P. flocculosa is an example of such glycolipid (Teichmann et al, 2007, 2011a,b).…”

Nitrogen Source Affects Glycolipid Production and Lipid Accumulation in the Phytopathogen Fungus <i>Ustilago maydis</i>

“…maydis is a phytopathogen basidiomycete that grows at the expenses of the nutrients given by the host. Although it is not an oily yeast, U. maydis is able to grow on oily substrates such as free fatty acids, and produce glycolipids upon nitrogen limitation [9] [11] [20] [21]. Our study confirms that under nitrogen-limitation U. maydis produced glycolipids differentially and that nitrogen starvation increases the accumulation of neutral lipids favoring the production of a single type of glycolipid (UA) as reported in the study of Nit2 control which is involved in nitrogen catabolite repression [22].…”

Production and characterization of a new glycolipid, mannosylerythritol lipid, from waste cooking oil biotransformation by Pseudozyma aphidis ZJUDM34

“…, including soybean oil, alkanes, glycerol, glucose, and xylose. (Arutchelvi & Doble, ; Morita, Fukuoka, Imura, & Kitamoto, ; Rau, Nguyen, Schulz et al, ). In addition, almost all vegetable oils (except palm oil and coconut oil) have been found to serve as a good carbon source for MELs by different Pseudozyma spp .…”