Antifungal activity of the bioactive substance from Bacillus atrophaeus strain HAB-5 and its toxicity assessment on Danio rerio

Rajaofera, Mamy Jayne Nelly; Jin, Pengfei; Fan, Yongmei; Sun, Qian; Huang, Weikang; Wang, W.B.; Shen, Hong; Zhang, S.; Lin, Chunhua; Liu, W.B.; Zheng, Fucong; Miao, Weiguo

doi:10.1016/j.pestbp.2017.06.006

Cited by 25 publications

(24 citation statements)

References 30 publications

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“…Further identification of Ba168 was confirmed by 16SrRNA analysis. The forward primer (5’-AGTTTGATCMTGGCTCAG-3’) and the reverse primer (5’-GGTTACCTTGTTACGACTT-3’) was used (11). The phylogenetic analysis was conducted using representatives of B. amyloliquefaciens strain SB 3297 (Genbank accession number(GAN): GU191913.1) (38), Bacillus vallismortis strain DSM 11031 (GAN: NR_024696.1) (39), B. subtilis subsp.…”

Section: Methodsmentioning

confidence: 99%

“…Biocontrol of soil-borne pathogens is preferred worldwide, because of the favorable biocontrol efficacy, if applied properly, and the urgent need to introduction environmental friendly alternatives to gradually reducing the use of harmful agrochemicals (9). So far, various P. nicotianae control strains, like Bacillus subtilis Tpb55 (10), Bacillus atrophaeus HAB-5 (11), non-pathogenic binucleate Rhizoctonia fungi (12), Trichoderma (13, 14), Brassica carinata pellets (15), Glomus mosseae and Pseudomonas fluorescens (16), have been evaluated. Among these biocontrol agents, P. fluorescens is one of the most studied PGPR (17), which contributed greatly to the understanding of the mechanisms that are involved in disease suppression.…”

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confidence: 99%

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Biocontrol of PGPR strain Bacillus amyloliquefaciens Ba168 against Phytophthora nicotianae on tobacco

Guo

Yuan

Luo

et al. 2019

Preprint

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Abstract： 9Tobacco black shank (TBS) caused by Phytophthora nicotianae is destructive to almost all 10 kinds of tobacco cultivars and is widespread in many tobacco-planted countries. Here, an isolated 11 plant growth-promoting rhizobacteria (PGPR) strain Ba168 is promise in biocontrol of TBS. In 12 vitro assays disclosed a strong P. nicotianae suppression activity and the field utilization potential 13 (FUP) by characterized the crude extract of culture filtrates of Ba168. P. nicotianae's growth was 14 inhibited by the crude extract at minimum inhibitory concentration (MIC) of 5μl/mL. Extracellular 15 conductivity, pH and the wet, dry weight of P. nicotianae's mycelia, were significantly different 16 after treated with different concentrations of the crude extract and the deformity and perforation of 17 treated P. nicotianae's hyphae can be observed in scanning electron microscope (SEM) analysis. 18Proteome characterizations of the crude extract were used as supplementary proofs that further 19 evaluated FUP of Ba168. We then identified strain Ba168 as B. amyloliquefaciens by its genetic 20 and phenotypic characteristics. Field assays comparatively evaluated TBS control efficacy of these 21PGPRs and agrochemicals. Pooling analysis of the results showed that the biocontrol efficacy of 22Ba168 preparation is only lower than Mixture of Propamocarb hydrochloride and Azoxystrobin 23 (MPA) but better than other tested subjects. Although the existence of differences in biocontrol 24 efficacy, PGPR preparations effectively reduced the disease index of tobacco. 25

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Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 99%

Biocontrol of PGPR strain Bacillus amyloliquefaciens Ba168 against Phytophthora nicotianae on tobacco

Guo

Yuan

Luo

et al. 2019

Preprint

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“…Twelve strains of fungal pathogens ( Supplementary Table 1) were selected from the Key Laboratory of Integrated Pest Management on Tropical Crops, including 10 strains of soilborne pathogens and 2 strains of common pathogens. The antagonism of HN03 toward the pathogens was assessed by measuring the inhibition of the growth rate using a method from Rajaofera et al (2017) with a slight modification. A 6 mm mycelial disk of a pathogenic fungus collected from the edge of an actively growing colony was placed into the center of a PDA (potato dextrose agar) plate.…”

Section: Testing For Antifungal Activity Against Fungal Pathogensmentioning

confidence: 99%

The Soil Nutrient Environment Determines the Strategy by Which Bacillus velezensis HN03 Suppresses Fusarium wilt in Banana Plants

Ying

et al. 2020

Front. Plant Sci.

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Biological control agents (BCAs) are considered as one of the most important strategies for controlling Fusarium wilt, and bioorganic fertilizer, in particular, has been extensively investigated. However, little is known regarding how a biocontrol microorganism affects the suppression mechanisms when combined with different amendments. In this study, a pot experiment was performed using banana plants to investigate the different mechanisms by which the biocontrol bacterium Bacillus velezensis HN03 (isolated from our laboratory) and amendments suppress Fusarium wilt. The incidence of banana wilt was decreased under HN03 and was reduced further when HN03 was combined with compost, particularly wormcast. In the suppression of Fusarium wilt, HN03 was found to influence the soil environment in various ways. HN03 increased the peroxidase level, which improves plant defense, and was highest when combined with wormcast, being 69 times higher than when combined with cow dung compost. The high accumulation of Mg and P in the “HN03 + wormcast” and Zn and Mn in the “HN03 + cow dung” treatments was negatively correlated with disease incidence. Furthermore, HN03 re-established the microbial community destroyed by the pathogen and further increased the level of suppression in the wormcast. HN03 also enhanced the functional traits of the soil, including defensive mechanism-related traits, and these traits were further enhanced by the combination of HN03 + wormcast.

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“…The isolate designed as strain HAB-5 was obtained from a healthy cotton field in Xinjiang province, P.R China. After isolation, strain HAB-5 was examined for morphological, physiological and biochemical characteristics (Rajaofera et al 2017(Rajaofera et al , 2019.…”

Section: Bacterial Strainmentioning

confidence: 99%

“…Despite the successful use of Bacillus species, reports about Bacillus atrophaeus application are rare. We have reported that B. atrophaeus strain HAB-5 can control a broad spectrum of crop disease-causing agents (Rajaofera et al 2017(Rajaofera et al , 2019. B. atrophaeus are diverse in terms of plant protection playing important role in crop yield reducing risks.…”

Section: Introductionmentioning

confidence: 99%

Bacillus atrophaeus HAB-5 secretion metabolites preventing occurrence of systemic diseases in tobacco plant

et al. 2019

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Finding synthetic pesticide alternatives for health and a healthy environment has become a crucial issue for scientific research. A number of studies have reported efficacy of Bacillus species on promoting plant development, as well as protecting plants against pathogen invasion, especially pathogenic fungi and bacteria. However, little was known about Bacillus species in controlling viral diseases. In this study, Bacillus atrophaeus strain HAB-5, isolated from cotton field, Xinjiang, China efficiently promoted the growth of tobacco plants. According to the results, the treatment with the strain HAB-5 increased the expression of NtEXP1 and NtEXP2. Then the Tobacco mosaic virus (TMV)/ Nicotiana tobacco system was employed to evaluate virus resistance induced by strain HAB-5. Tobacco leaves were treated with antimicrobial metabolites of strain HAB-5 (1 mg/mL), and 12 h later the treated leaves were challenged with TMV via rub-inoculation. The results showed that disease symptoms were obviously compromised by tobacco leaves treated with strain HAB-5, and the viral accumulation level was reduced extensively. Moreover, it was found that the signaling regulatory gene (NPR1), defense genes (PR-1a, PR-1b, Chia5), and hypertensive response related genes (Hsr203J, Hin1) were up-regulated in plants treated with the metabolites. Altogether, these accumulated results strongly support strain HAB-5 to be a biological controlling agent against TMV.

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Antifungal activity of the bioactive substance from Bacillus atrophaeus strain HAB-5 and its toxicity assessment on Danio rerio

Cited by 25 publications

References 30 publications

Biocontrol of PGPR strain Bacillus amyloliquefaciens Ba168 against Phytophthora nicotianae on tobacco

Biocontrol of PGPR strain Bacillus amyloliquefaciens Ba168 against Phytophthora nicotianae on tobacco

The Soil Nutrient Environment Determines the Strategy by Which Bacillus velezensis HN03 Suppresses Fusarium wilt in Banana Plants

Bacillus atrophaeus HAB-5 secretion metabolites preventing occurrence of systemic diseases in tobacco plant

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