Antifungal activity of biosynthesized silver nanoparticles from Candida albicans on the strain lacking the CNP41 gene

Dhabalia, Darshan; Ukkund, Shareefraza J.; Syed, Usman Taqui; Uddin, Wasim; Kabir, M. Anaul

doi:10.1088/2053-1591/abcc83

Cited by 10 publications

(4 citation statements)

References 37 publications

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“…8), thereby suggesting that Orf19.3120 may have a minor role in drug resistance. Conversely, the strain lacking this ORF was sensitive to fluconazole in the presence of silver nanoparticles [48].…”

Section: Discussionmentioning

confidence: 99%

Systematic truncations of chromosome 4 and their responses to antifungals in Candida albicans

Uddin

Dhabalia

Prakash

et al. 2021

Journal of Genetic Engineering and Biotechnology

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Background Candida albicans is an opportunistic human fungal pathogen responsible for superficial and systemic life-threatening infections. Treating these infections is challenging as many clinical isolates show increased drug resistance to antifungals. Chromosome (Chr) 4 monosomy was implicated in a fluconazole-resistant mutant. However, exposure to fluconazole adversely affects Candida cells and can generate numerous mutations. Hence, the present study aimed to truncate Chr4 and challenge the generated Candida strains to antifungals and evaluate their role in drug response. Results Herein, Chr4 was truncated in C. albicans using the telomere-mediated chromosomal truncation method. The resulting eight Candida strains carrying one truncated homolog of Chr4 were tested for response to multiple antifungals. The minimal inhibitory concentration (MIC) for these strains was determined against three classes of antifungals. The MIC values against fluconazole, amphotericin B, and caspofungin were closer to that of the wild type strain. Microdilution assay against fluconazole showed that the mutants and wild type strains had similar sensitivity to fluconazole. The disc diffusion assay against five azoles and two polyenes revealed that the zones of inhibition for all the eight strains were similar to those of the wild type. Thus, none of the generated strains showed any significant resistance to the tested antifungals. However, spot assay exhibited a reasonably high tolerance of a few generated strains with increasing concentrations of fluconazole. Conclusion This analysis suggested that Chr4 aneuploidy might not underlie drug resistance but rather drug tolerance in Candida albicans.

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Section: Discussionmentioning

confidence: 99%

Systematic truncations of chromosome 4 and their responses to antifungals in Candida albicans

Uddin

Dhabalia

Prakash

et al. 2021

Journal of Genetic Engineering and Biotechnology

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“…Disc diffusion test was performed to evaluate the antifungal activity as described (Dhabalia et al 2020 ). Sterile 6-mm disks were impregnated in the agar plates.…”

Section: Methodsmentioning

confidence: 99%

Application of agro-waste-mediated silica nanoparticles to sustainable agriculture

Goswami

Mathur

2022

Bioresour. Bioprocess.

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Use of green agronomic techniques for plant development and crop protection is essential for environmental sustainability. The current research investigates a more efficient and long-term technique of manufacturing silica nanoparticles (SiO2 NPs) from agricultural waste (sugarcane bagasse and corn cob). SiO2 NPs were synthesized by calcinations of waste residues in muffle furnace with varying temperatures (400–1000 °C)/2 h in the present of static air. Field emission scanning electron microscopy (FESEM), Fourier transmission infrared spectroscopy (FTIR), X-ray diffraction (XRD), and energy dispersive X-ray spectroscopy (EDX) were used to characterize SiO2 NPs and assessed for their antifungal activity simultaneously investigated the effects of various concentrations of produced SiO2 NPs on Eruca sativa (E. sativa) physiological and biochemical. With SiO2 NPs treatment at 1000 µg L−1 concentration, the seed germination rate was found to be up to 95.5%, and growth characteristics were enhanced compared to control. Accordingly, the ones treated with SiO2 NPs grew better than the control ones. The treatment of plant with SiO2 NPs (500 μg L−1) increased the protein content by 14.8 mg g−1, and chlorophyll level was also increased by 4.08 mg g−1 in leaves compared to untreated plant. Disc diffusion experiment was conducted to test the efficiency of SiO2 NPs against Fusarium oxysporum and Aspergillus niger for antifungal activities. Highest mycelia growth inhibition was obtained with 73.42% and 58.92% for F. oxysporum and A. niger, respectively. The result shows that the SiO2 NPs have a favorable effect on E. sativa growth and germination, enhancing plant production which helps to improve the sustainable agriculture farming and acting as a possible antifungal agent against plant pathogenic fungi. Graphical Abstract

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“…Recently synthesized nanostructures of zinc, manganese, and thioacetamide have been found to possess antifungal activity against pathogenic fungal species [42]. In recent studies, ZnO nanoparticles were synthesized that exhibited antifungal activity [43][44][45][46][47]. In low concentrations, silver nanoparticles were less cytotoxic than zinc oxide nanoparticles and represent an alternative antimicrobial compound against A. hydrophila, A. salmonicida and A. invadans [48].…”

Section: Zinc Nanostructures and Their Hybridsmentioning

confidence: 99%

Anti-fungal therapy via incorporation of nanostructures: A systematic review for new dimensions

Younus¹,

Khan²,

Maqbool³

et al. 2022

Phys. Scr.

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Background: Fungal infections are one of the most prevailing worldwide public health issues. Despite the commercially available antifungal drugs, side effects associated with these agents and resistance may lead to the development of antifungal drugs by using advanced technology. Main body of the abstract: In the current era, nanotechnology is one of the most predominant techniques with massive advantages in the field of medicines and medical sciences. In the last two decades, various nanostructures were developed based on metals, plants, and microorganisms. The aim of the review is to discuss all original researches and to review articles of the last two decades, including earlier and recent development in different types of nanostructures as an antifungal therapy. Published papers in the English language were searched and reviewed from the online databases from January 2007 till May 2021. The search engines included were PubMed, Google, Google scholar, and Sciencedirect. In all the databases, the keywords “fungi”, “antifungal”, “nanomaterials”, “nanostructures” and “nanotechnology” were used for literature search. Short conclusion: The innovative nanostructures are vital for the treatment of different strains of fungal infections i.e. from minor nail infections to major systemic fungal infections. Additionally, nanostructures also enhance the efficacy, solubility, penetration, and drug release to the targeted site of conventional antifungal agents.

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Antifungal activity of biosynthesized silver nanoparticles from Candida albicans on the strain lacking the CNP41 gene

Cited by 10 publications

References 37 publications

Systematic truncations of chromosome 4 and their responses to antifungals in Candida albicans

Systematic truncations of chromosome 4 and their responses to antifungals in Candida albicans

Application of agro-waste-mediated silica nanoparticles to sustainable agriculture

Anti-fungal therapy via incorporation of nanostructures: A systematic review for new dimensions

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