Anticancer Activity Test for Extracts of Sarang Semut Plant (Myrmecodya pendens) to HeLa and MCM-B2 Cells

Abstract

The aim of this study is to investigate anticancer activity of methanol extract (ethylacetate, n-buthanol and water partitions) and water extract from Sarang semut (local name), Myrmecodya pendens which is one of Rubiaceae family. Within Papua area (Indonesia), this medicinal plant has been used traditionally as alternative treatment for ulcer, tumor and cancer. In this study, the extracts of this plant were tested for their activities in some cancer cells (HeLa and MCM-B2 cell). The result showed that water extract of this plant has better anti cancer activity compared to other extracts. The IC50 value of water extract A is 27.61 ppm (HeLa) and 54.57 ppm (MCM-B2), while water extract B is 29.36 ppm (HeLa) and 74.20 ppm (MCM-B2). Our study concluded that polar extract (water) exhibited higher anticancer activity than non-polar extracts (ethylacetate and n-buthanol).

The N-Hexane Fraction of Myrmecodia Pendans Inhibits Cell Survival and Proliferation in Colon Cancer Cell Line

“…A phytochemical study of Sarang Semut reported active phenolic compounds found in ethanol 70% extract and boiling water extract including flavonoids, tannin, phenolic, and alkaloid [7]. Other study showed that water extract of Sarang Semut contains flavonoid compounds, saponin, glycoside, and tannin; while the n-butanol extract contains flavonoids, tannins, and quinons; and the ethyl acetate extract contains only flavonoids and tannins [8]. Flavonoid compounds were also found in ethyl acetate, methanol, as well as ethanol extract and also in ethyl acetate fraction as well as n-hexane fraction of Sarang Semut [22,23].…”

“…Sarang Semut is a member of Rubiaceae family, which two genus are naturally become ant nest (Sarang Semut) including Myrmecodia (45 species) and Hypnophytum (26 species) [8]. Some studies have proven the efficacy of Sarang Semut in different cancer entities, including breast, uterus, tongue, and oral cancer [8][9][10].…”

“…Chemical screening tests showed that Sarang Semut contains flavonoid and tannin [7][8][9]. Many mechanisms of action of flavonoid have been revealed, such as carcinogen inactivation, antiproliferation, apoptotic induction, antioxidants, inhibition of angiogenesis as well as cell cycle or combination of these mechanisms [12].…”

Eco-friendly green inhibitor of mild steel in 3,5% NaCl solution by Sarang Semut (Myrmecodia Pendans) extract

“…Myrmecodia Pendans (in Indonesia called Sarang Semut) is a plant that has a high antioxidant activity [3]. In previous studies, sarang semut shown to contain flavanoids after tested using HPLC.…”

The Effect of Ethanol Extract Of Sarang Semut Plant (Hypnophytum Formicarum) to Granulation Tissue for Wound Healing After Teeth Extraction Experimental Research on Marmot (Cavia cobaya)

“…11,12 Flavonoids as antibacterial, antiinflammatory and antioxidant, antibacterial saponins, tannins as a hemostatic and astringent and alkaloids as an analgesic. 13,14 The aim of this study was to determine the effect of ethanol extract of the root of sarang semut as formulation of granulation tissue on wound healing after tooth extraction.…”