The N-Hexane Fraction of Myrmecodia Pendans Inhibits Cell Survival and Proliferation in Colon Cancer Cell Line

Bashari, Muhammad Hasan; Hidayat, S.; Ruswandi, Yasmin Anissa Robles; Putri, Tenny; Qomarilla, Nurul; Dwiwina, Resti Gradia; Kurnia, Dikdik; Satari, Mieke Hemiawati; Huda, Fathul

doi:10.22159/ijpps.2018v10i1.21882

Cited by 10 publications

(16 citation statements)

References 23 publications

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“…This grounded M. pendens was later macerated with methanol for 2-3 days followed by filtered then evaporated and dried into dry powder. This methanol extract was partitioned using n-hexane or ethyl acetate to yield n-hexane fraction or ethyl acetate fraction (Bashari, et al, 2018).…”

Section: Preparation Of Extract and Fractions Of Myrmecodia Pendensmentioning

confidence: 99%

“…In particular cancer research, Sarang Semut plant has been shown cytotoxic effects in different cancer cell lines from different type of extractions (Soeksmanto, et al, 2010;Fatmawati, Puspitasari & Yusuf, 2011;Rifayani, et al, 2015;Bashari, et al, 2018). Sarang Semut extract has been shown to have a cytotoxic effect in HeLa and MCM-B2 cells (Soeksmanto, et al, 2010;Fatmawati, Puspitasari & Yusuf, 2011).…”

Section: Introductionmentioning

confidence: 99%

“…Sarang Semut extract has been shown to have a cytotoxic effect in HeLa and MCM-B2 cells (Soeksmanto, et al, 2010;Fatmawati, Puspitasari & Yusuf, 2011). The n-hexane fraction of M. pendens has been appraised to have the strongest cytotoxic activity compared with other extracts of M. pendens on colon cancer cells (Bashari, et al, 2018). Previous studies showed ethyl acetate fraction, water extract as well as ethanol extract of Sarang Semut trigger BC and CC cell death (Fatmawati, Puspitasari & Yusuf, 2011;Mudjahid & Budiani, 2015;Andriani, et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

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Inhibition Capacity of the n-Hexane Fraction of Myrmecodia pendens as a Potential Anti-Cancer in Breast and Cervical Cancer: In Vitro Study

Bashari

Yuniarti

Putri

et al. 2020

Indones J Cancer Chemoprevent

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Breast cancer (BC) and cervical cancer (CC) have a high prevalence and mortality rate worldwide. Despite the availability of advanced treatment, resistance to conventional chemotherapies has emerged. Myrmecodia pendens, one of the species of Sarang Semut (local name), possess a potential of antitumor effects by inducing cell death different cancer cell entities. This study aimed to assess anti-tumor activities of n-hexane fraction of M. pendens in inhibiting cell survival and cell migration in BC and CC cells. M. pendens was extracted in methanol then fractionated using n-hexane or ethyl acetate. BC cells including MCF-7 (luminal A), HCC-1954 (HER2+) cells and CC Hela cells were treated with M. pendens extracts to evaluate cytotoxic activity using 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide (MTT) assay as well as anti-cell migration using scratch assay. We also analyzed inhibitory concentration 50 (IC50) of n-hexane fraction in BC and CC cells. We started with comparing cytotoxicity activities of methanol extract, ethyl acetate and n-hexane fractions of M. pendens. Data showed that the n-hexane fraction was the most potent inducing BC cell death. Therefore, we used the n-hexane fraction for further experiments. Interestingly, IC50 of this fraction in HCC-1954 and Hela cells were lower than in MCF-7 cells, 16; 13 and 60 ppm, respectively. Moreover, the low concentrations of n-hexane fraction inhibited HeLa cells migration, compared to control group (p<0.05). The n-hexane fraction of M. pendens shows promising anti-cancer agent, by inhibiting BC and CC cell survival as well as inhibiting CC cells migration.Keywords: breast cancer, cervical cancer, MTT assay, Sarang Semut, scratch assay

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Section: Preparation Of Extract and Fractions Of Myrmecodia Pendensmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Inhibition Capacity of the n-Hexane Fraction of Myrmecodia pendens as a Potential Anti-Cancer in Breast and Cervical Cancer: In Vitro Study

Bashari

Yuniarti

Putri

et al. 2020

Indones J Cancer Chemoprevent

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“…Some mechanisms of action of flavonoids have been identified, such as inactivation of carcinogens, antiproliferation, cell cycle inhibition, apoptotic induction, inhibition of angiogenesis, antioxidants, or the combination of such mechanisms [33].…”

Section: Jainab and Rajamentioning

confidence: 99%

In-Vitro Cytotoxic and Apoptosis Study of Chemical Constituents of Clerodendrum Phlomidis Leaf on McF-7 Breast and a-549 Lung Cancer Cell Lines.

Raja

2018

Asian J Pharm Clin Res

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Objective: Cancer is a molecularly heterogeneous diseaseand the leading cause of death worldwide. The methanol extract of Clerodendrum phlomidis leaf has been reported for cytotoxicity. Hence, the current investigation was planned to evaluate the cytotoxic activity of the chemical constituents isolated from the methanol extract of the C. phlomidis leaf against the Michigan cancer foundation-7 (MCF-7) breast cancer and adenocarcinomic human alveolar basal epithelial cells (A549) lung cancer cell lines by the apoptotic study.Methods: Cytotoxic activity of the chemical constituents of the methanol extract of the C. phlomidis leaf was evaluated by the 3-(4,5-dimethylthiazol-2-yl)-5-diphenyltetrazolium bromide (MTT) assay against breast cancer (MCF-7) and lung cancer (A549) cell lines. The MCF-7 and A549 cell lines were tested at different concentrations to determine 50% of growth inhibition (inhibitory concentration [IC 50 ]) by MTT assay. Apoptosis of nuclei was detected by 4',6-diamidino-2-phenylindole staining assay. Results:In MCF-7 breast cancer cell line study, Compounds 6 and 9 exhibited good cytotoxic activity with an IC 50 value of 83.80 and 75.16 µg/ml, respectively. In A549 lung cancer cell line, again Compounds 6 and 9 exhibited good cytotoxic activity with an IC 50 value of 84.46 and 78.60 µg/ml, respectively. Percentage of apoptosis induced by the Compounds 6 and 9 in the MCF-7 cancer cells was found to be 74.50 and 85.48, respectively. Conclusion:The results of the current study prove that the Compounds 6 and 9 are potential agents for breast and lung cancer. In future research, these potential agents can further be evaluated by animal studies and their mechanism of action apart from the induction of apoptosis of the cancer cells can be determined.

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“…It is used empirically as a medical plant to treat various diseases. [11][12][13] This plant is valuable as an alternative endodontic irrigant and shows minimal side effects. Soraya (2016) found that M. pendens extract has an antibacterial effect against E. faecalis.…”

Section: Introductionmentioning

confidence: 99%

Antimicrobial Efficacy of Myrmecodia pendens Extract and Fraction Combination against Enter action Combination against Enterococcus faecalis A ococcus faecalis ATCC 29212

Kuswandani¹,

Satari²,

Maskoen³

2019

J Dent Indones

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Enterococcus faecalis can withstand harsh environmental conditions in the root canal and cause a secondary infection. Myrmecodia pendens is an herbal medicine rich in polyphenol compounds that have antibacterial, antioxidant, and anticancer properties. Objectives: To analyze the effects of M. pendens fraction combination on the sensitivity of E. faecalis. Methods: The minimum inhibitory concentration (MIC) was determined using a serial microdilution method, and the minimum bactericidal concentration (MBC) was determined by adding the test sample to sterile Mueler Hinton agar medium. Results: The MIC of combination 1 (hexane-ethyl acetate, HE) was 0.049 mg/mL, whereas those of combinations 2 (hexane-water, HA) and 3 (ethyl acetate-water, EA) were 0.098 and 0.390 mg/ mL, respectively. The MIC of NaOCl was 0.390 mg/mL, and that of methanol extract was 0.390 mg/mL. The MBC of combination 1 (HE) was 12.50 mg/mL, whereas that of combinations 2 (HA) and 3 (EA) was 50 mg/mL. The MBC of NaOCl was 25 mg/mL and that of methanol extract was 50 mg/mL against E. faecalis. This study showed that fraction combinations increase the antibacterial effect of M. pendens against E. faecalis ATCC 29212. Conclusion: The HE fraction combination showed the best effect against E. faecalis and can be developed as an alternative endodontic irrigant.

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The N-Hexane Fraction of Myrmecodia Pendans Inhibits Cell Survival and Proliferation in Colon Cancer Cell Line

Cited by 10 publications

References 23 publications

Inhibition Capacity of the n-Hexane Fraction of Myrmecodia pendens as a Potential Anti-Cancer in Breast and Cervical Cancer: In Vitro Study

Inhibition Capacity of the n-Hexane Fraction of Myrmecodia pendens as a Potential Anti-Cancer in Breast and Cervical Cancer: In Vitro Study

In-Vitro Cytotoxic and Apoptosis Study of Chemical Constituents of Clerodendrum Phlomidis Leaf on McF-7 Breast and a-549 Lung Cancer Cell Lines.

Antimicrobial Efficacy of Myrmecodia pendens Extract and Fraction Combination against Enter action Combination against Enterococcus faecalis A ococcus faecalis ATCC 29212

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