Antibody and DNA dual-labeled gold nanoparticles: Stability and reactivity

Qiao, Fei-yan; Li, Jun; Li, Furong; Kong, Xiao-Li; Zhang, Hao‐Li; Zhou, Haixia

doi:10.1016/j.apsusc.2007.10.046

Cited by 31 publications

(9 citation statements)

References 13 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Gold nanoparticles were chosen as they are biologically compatible, provide a large surface area on which to couple multiple probes and allow the use of facile gold-thiol chemistry [32], [33], [34] and [35]. Recently, AuNPs have been co-functionalized with antibodies and DNA for a number of applications [36], [37] and [38]. The 15 nm AuNPs used in this study were functionalized with Synagis® antibodies (humanized monoclonal antibodies targeted to the RSV fusion protein) to bind to antigen.…”

Section: Resultsmentioning

confidence: 99%

Detection of respiratory syncytial virus using nanoparticle amplified immuno-polymerase chain reaction

Perez

Vargis

Russ

et al. 2011

Analytical Biochemistry

View full text Add to dashboard Cite

In traditional immuno-PCR a single antibody recognition event is associated with one to three DNA tags, which are subsequently amplified by PCR. Here, we describe a nanoparticle amplified immuno-PCR (NPA-IPCR) assay that combines antibody recognition of ELISA with a 50-fold nanoparticle valence amplification step prior to tag amplification by PCR. The assay detects a respiratory syncytial virus (RSV) surface protein using an antibody bound to a 15 nm gold nanoparticle co-functionalized with thiolated DNA complementary to a hybridized to 76-base tag DNA with a tag DNA to antibody ratio of 50 to 1. The presence of virus particles triggers the formation of a “sandwich” complex comprised of the gold nanoparticle construct, virus and an antibody functionalized magnetic particle used for extraction. After extraction, DNA tags are released by heating to 95°C and detected via real-time PCR. The limit of detection of the assay was compared to ELISA and RT-PCR using RSV infected HEp-2 cell extracts. NPA-IPCR showed a ~4000-fold improvement in the limit of detection compared to ELISA and a 4-fold improvement compared to viral RNA extraction followed by traditional RTPCR. NPA-IPCR offers a viable platform for the development of an early-stage diagnostics requiring an exceptionally low limit of detection.

show abstract

Section: Resultsmentioning

confidence: 99%

Detection of respiratory syncytial virus using nanoparticle amplified immuno-polymerase chain reaction

Perez

Vargis

Russ

et al. 2011

Analytical Biochemistry

View full text Add to dashboard Cite

show abstract

“…DNA and antibodies are vulnerable to lose its bioactivities because of the change of molecular conformation and surrounding environment in the synthetic process293031. Therefore, it is necessary to assess the stability of the AuNPs probes.…”

Section: Resultsmentioning

confidence: 99%

A Competitive Bio-Barcode Amplification Immunoassay for Small Molecules Based on Nanoparticles

Jin

Chen

et al. 2016

Sci Rep

View full text Add to dashboard Cite

A novel detection method of small molecules, competitive bio-barcode amplification immunoassay, was developed and described in this report. Through the gold nanoparticles (AuNPs) probe and magnetic nanoparticles (MNPs) probe we prepared, only one monoclonal antibody can be used to detect small molecules. The competitive bio-barcode amplification immunoassay overcomes the obstacle that the bio-barcode assay cannot be used in small molecular detection, as two antibodies are unable to combine to one small molecule due to its small molecular structure. The small molecular compounds, triazophos, were selected as targets for the competitive bio-barcode amplification immunoassay. The linear range of detection was from 0.04 ng mL−1 to 10 ng mL−1, and the limit of detection (LOD) was 0.02 ng mL−1, which was 10–20 folds lower than ELISA (Enzyme Linked Immunosorbent Assay). A practical application of the proposed immunoassay was evaluated by detecting triazophos in real samples. The recovery rate ranged from 72.5% to 110.5%, and the RSD was less than 20%. These results were validated by GC-MS, which indicated that this convenient and sensitive method has great potential for small molecular in real samples.

show abstract

“…The conjugation of a range of diverse biological molecules to plain gold nanospheres (35-80 nm) to produce probes in molecular biology assays has been investigated extensively [13][14][15]. These nanospheres are easy to produce and can be tuned to interact with specific wavelengths in the electromagnetic spectrum.…”

Section: Plain Gold Nanospheresmentioning

confidence: 99%

Recent Innovations in Antibody-Mediated, Targeted Particulate Nanotechnology and Implications for Advanced Visualisation and Drug Delivery

Fay¹,

Scott²,

McCarron³

2010

CNANO

View full text Add to dashboard Cite

Research into the targeting of drug substances to a specific disease site has enjoyed sustained activity for many decades. The reason for such fervent activity is the considerable clinical advantages that can be gained when the delivery system plays a pivotal role in determining where the drug is deposited. When compared to conventional formulations where no such control exists, such as parenteral and oral systems, the sophisticated targeting device can reduce side effects and limit collateral damage to surrounding normal tissue. No more so is this important than in the area of oncology when dose-limiting side effects are often encountered as an ever present difficulty. In this review, the types of colloidal carrier commonly used in targeted drug delivery are discussed, such as gold and polymeric colloids. In particular, the process of attaching targeting capabilities is considered, with reference to antibody technologies used as the targeting motifs. Nanotechnology has brought together a means to carry both a drug and targeting ligand in self-contained constructs and their applications to both clinical therapy and diagnosis are discussed.

show abstract

Antibody and DNA dual-labeled gold nanoparticles: Stability and reactivity

Cited by 31 publications

References 13 publications

Detection of respiratory syncytial virus using nanoparticle amplified immuno-polymerase chain reaction

Detection of respiratory syncytial virus using nanoparticle amplified immuno-polymerase chain reaction

A Competitive Bio-Barcode Amplification Immunoassay for Small Molecules Based on Nanoparticles

Recent Innovations in Antibody-Mediated, Targeted Particulate Nanotechnology and Implications for Advanced Visualisation and Drug Delivery

Contact Info

Product

Resources

About