Detection of respiratory syncytial virus using nanoparticle amplified immuno-polymerase chain reaction

Perez, Jonas W.; Vargis, Elizabeth; Russ, Patricia K.; Haselton, Frederick R.; Wright, David W.

doi:10.1016/j.ab.2010.11.033

Cited by 59 publications

(60 citation statements)

References 43 publications

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“…Some of these immuno-PCR assays use nanoparticles and are called nanoparticle-amplified immuno-PCR 142,143 . In a traditional immuno-PCR, a single antibody recognition event is associated with one to three DNA oligo tags which are subsequently amplified by PCR.…”

Section: Respiratory Syncytial Virusmentioning

confidence: 99%

“…In a traditional immuno-PCR, a single antibody recognition event is associated with one to three DNA oligo tags which are subsequently amplified by PCR. Perez et al developed a nanoparticle-amplified immuno-PCR that combines antibody recognition of ELISA with a 50-fold nanoparticle valence amplification step prior to the reporter amplification with PCR 143 . In this assay antibody to RSV surface proteins are coupled to 15 nm gold particles which are also tagged with DNA oligos at a DNA/antibody ratio of 50:1.…”

Section: Respiratory Syncytial Virusmentioning

confidence: 99%

See 1 more Smart Citation

Molecular diagnosis of respiratory virus infections

Mahony¹,

Petrich²,

Śmieja³

2011

Critical Reviews in Clinical Laboratory Sciences

183

181

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The appearance of eight new respiratory viruses, including the SARS coronavirus in 2003 and swine-origin influenza A/H1N1 in 2009, in the human population in the past nine years has tested the ability of virology laboratories to develop diagnostic tests to identify these viruses. Nucleic acid based amplification tests (NATs) for respiratory viruses were first introduced two decades ago and today are utilized for the detection of both conventional and emerging viruses. These tests are more sensitive than other diagnostic approaches, including virus isolation in cell culture, shell vial culture (SVC), antigen detection by direct fluorescent antibody (DFA) staining, and rapid enzyme immunoassay (EIA), and now form the backbone of clinical virology laboratory testing around the world. NATs not only provide fast, accurate and sensitive detection of respiratory viruses in clinical specimens but also have increased our understanding of the epidemiology of both new emerging viruses such as the pandemic H1N1 influenza virus of 2009, and conventional viruses such as the common cold viruses, including rhinovirus and coronavirus. Multiplex polymerase chain reaction (PCR) assays introduced in the last five years detect up to 19 different viruses in a single test. Several multiplex PCR tests are now commercially available and tests are working their way into clinical laboratories. The final chapter in the evolution of respiratory virus diagnostics has been the addition of allelic discrimination and detection of single nucleotide polymorphisms associated with antiviral resistance. These assays are now being multiplexed with primary detection and subtyping assays, especially in the case of influenza virus. These resistance assays, together with viral load assays, will enable clinical laboratories to provide physicians with new and important information for optimal treatment of respiratory virus infections.

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Section: Respiratory Syncytial Virusmentioning

confidence: 99%

Section: Respiratory Syncytial Virusmentioning

confidence: 99%

Molecular diagnosis of respiratory virus infections

Mahony¹,

Petrich²,

Śmieja³

2011

Critical Reviews in Clinical Laboratory Sciences

183

181

View full text Add to dashboard Cite

show abstract

“…When the antigen is present in a complex protein mix and analyzed in a sandwich assay, Nano-i PCR and i PCR usually detect the antigen with 1-3 orders higher sensitivity than ELISA (Perez et al 2011). The basis of this system which uses larger magnetic micro particles (MMPs) to detect molar concentrations of serum proteins is represented in Fig.…”

Section: Dna-based Advanced Materialsmentioning

confidence: 99%

In-vitro nanodiagnostic platform through nanoparticles and DNA-RNA nanotechnology

Chan

2015

Appl Microbiol Biotechnol

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Nanocomposites containing nanoparticles or nanostructured domains exhibit an even higher degree of material complexity that leads to an extremely high variability of nanostructured materials. This review introduces analytical concepts and techniques for nanomaterials and derives recommendations for a qualified selection of characterization techniques for specific types of samples, and focuses the characterization of nanoparticles and their agglomerates or aggregates. In addition, DNA nanotechnology and the more recent newcomer RNA nanotechnology have achieved almost an advanced status among nanotechnology researchers¸ therefore, the core features, potential, and significant challenges of DNA nanotechnology are also highlighted as a new discipline. Moreover, nanobiochips made by nanomaterials are rapidly emerging as a new paradigm in the area of large-scale biochemical analysis. The use of nanoscale components enables higher precision in diagnostics while considerably reducing the cost of the platform that leads this review to explore the use of nanoparticles, nanomaterials, and other bionanotechnologies for its application to nanodiagnostics in-vitro.

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“…The DNA tags take advantage of the inherent function of nucleic acids to store information, thereby functioning as “biobarcodes.” The sensitivity of IPCR has been further enhanced with the use of AuNPs to mediate the association of many DNA biobarcode tags with a single target recognition element. 44 …”

Section: Dna Biobarcode Tag-functionalized Goldmentioning

confidence: 99%

“…44–46 Anchored to a solid phase, such as a magnetic particle or the bottom of a 96-well plate, a second capture moiety is used to isolate the probe from the analyte solution. The capture moiety on the AuNP binds a different region of the target biomarker, forming a sandwich structure.…”

Section: Dna Biobarcode Tag-functionalized Goldmentioning

confidence: 99%

Design, Synthesis, and Characterization of Nucleic-Acid-Functionalized Gold Surfaces for Biomarker Detection

et al. 2011

Self Cite

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Nucleic acid-functionalized gold surfaces have been used extensively for the development of biological sensors. The development of an effective biomarker detection assay requires careful design, synthesis and characterization of probe components. In this feature article, we describe fundamental probe development constraints and provide a critical appraisal of the current methodologies and applications in the field. We discuss critical issues and obstacles that impede the sensitivity and reliability of the sensors to underscore the challenges that must be met to advance the field of biomarker detection.

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Detection of respiratory syncytial virus using nanoparticle amplified immuno-polymerase chain reaction

Cited by 59 publications

References 43 publications

Molecular diagnosis of respiratory virus infections

Molecular diagnosis of respiratory virus infections

In-vitro nanodiagnostic platform through nanoparticles and DNA-RNA nanotechnology

Design, Synthesis, and Characterization of Nucleic-Acid-Functionalized Gold Surfaces for Biomarker Detection

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