Antibiotics modulate biofilm formation in fish pathogenic isolates of atypical Aeromonas salmonicida

Abstract

Aeromonas salmonicida is the aetiological agent of furunculosis, a potentially lethal infection of many species of fish that manifests typically

Aeromonas veronii virulence and adhesion attenuation mediated by the gene aodp

“…In this study, the adhesion detection on EPCs showed that deletion of the aodp gene increased the ability of the strain to adhere to cells, which was significantly different from the wild strain A. veronii TH0426 ( p < .05). The experiment results were consistent with the above theory and indicated that bacterial adhesion was necessary for biofilm formation (Desbois et al., 2020; Dias et al., 2018; Freire et al., 2019). The LD 50 tests of zebrafish and mice showed a significant increase in LD 50 values and a notable decrease in the virulence of the △‐ aodp strain compared with the wild strain ( p < .01).…”

“…At the same time, the △‐ aodp strain showed an enhanced biofilm‐forming ability compared with A. veronii TH0426. Studies have shown that the generation of bacterial biofilm led to stronger drug resistance and stress resistance (Desbois et al., 2020). RNA‐seq results showed that the deletion of the gene aodp significantly affected 3 genes related to extracellular polysaccharide produced, among which B565_RS10920 and B565_RS10960 genes encoding glycosyltransferase family proteins and B565_RS14415 genes encoding glycobiosynthetic protein genes were up‐regulated.…”

Virulence and antimicrobial resistance potential ofAeromonasspp. associated with shellfish

“…Chenia and Duma (2017) reported that biofilm formation could be affected by available nutrients rather than temperature changes. Biofilm formation was reported in A. salmonicida recovered from finfish in United Kingdom (Desbois et al 2020). Aeromonas veroni isolates were recovered from shrimp in China (Liu et al 2020) and A. salmonicida, A. veroni, A. enteropelogenes, A. caviae and A.allosaccarophila were isolated from hard shell mussels (Hossain et al 2020).…”

“…Biofilm formation was reported in A. salmonicida recovered from finfish in United Kingdom (Desbois et al . 2020). Aeromonas veroni isolates were recovered from shrimp in China (Liu et al .…”

The effect of piscidin antimicrobial peptides on the formation of Gram‐negative bacterial biofilms

“…Recent in vitro work has demonstrated that the presence of different teleost fish mucus extracts upregulates pathways associated with robust F. columnare biofilm development (Lange et al., 2018, 2019). The persistence of A. hydrophila and F. columnare within production systems could lead to continuous disease problems (Hossain et al., 2014; Mohammed & Arias, 2014), as well as contribute to the potential overuse of therapeutics and, ultimately, to an increased incidence of antibiotic resistance among pathogens (Desbois et al., 2020; Manyi‐Loh et al., 2018).…”