Virulence and antimicrobial resistance potential of<i>Aeromonas</i>spp. associated with shellfish

Silva, L.A.D.S. De; Wickramanayake, M.V.K.S.; Heo, Gang‐Joon

doi:10.1111/lam.13489

Cited by 30 publications

(12 citation statements)

References 81 publications

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“…Temperature-induced changes in the gut microbiota may also lead to increase in pathogenic microbiota ( Fontaine et al, 2018 ). Aeromonas is a gram-negative facultative anaerobic bacterium, most of which have been identified as pathogenic to some organisms, including fish ( Dar et al, 2016 ) and shellfish ( Chen J. S. et al, 2021 ; De Silva et al, 2021 ). The relative abundance of Aeromonas in the high temperature group was significantly higher than that in the low temperature and the control groups ( Figure 7C ).…”

Section: Discussionmentioning

confidence: 99%

Effect of long-term temperature stress on the intestinal microbiome of an invasive snail

Qian

Gao

et al. 2022

Front. Microbiol.

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The gut microbiome is vital to the physiological and biochemical functions of the host, and changes in the composition of these microbial communities may affect growth and adaptability to the environment. Pomacea canaliculata is an invasive freshwater snail which has become a serious agricultural pest. Temperature adaptation is considered an important reason for the widespread distribution of this species. To date, the contribution of the gut microbes to host fitness of P. canaliculata during long-term temperature stress is not well understood. In this study, the morphological changes and intestinal microbiome of P. canaliculata under long-term stress at low temperature (15°C) and high temperature (35°C) were investigated with laboratory experiments. Compared with control group (25°C), the alpha diversity increased and pathogenic bacteria enriched changed under high and low temperature stress. The effect of high temperature stress on the intestinal microbiome of P. canaliculata was more significant than that of low temperature stress. A sustained high temperature environment led to an increase in the abundance of pathogenic bacteria, such as Aeromonas and Enterobacter, and a decrease in the abundance of immune-related bacteria such as Bacteroidetes, Firmicutes, and Lactococcus. These intestine microbiome changes can increase the risk of diseases like intestinal inflammation, and lead to more deaths at high temperature environments. In addition, with the extension of stress time from 14 to 28 days, the beneficial bacteria such as Bacteroidetes, Firmicutes, and Lactococcus were significantly enriched, while potential pathogenic bacteria such as Pseudomonas, Acinetobacter, Shivalella, and Flavobacterium decreased, suggesting that intestinal microbiota may play an important role in host response to heat stress. These results are consistent with previously reported results that the survival rate of both male and female P. canaliculata no longer significantly reduced after 21 days of high temperature stress, suggesting that the surviving P. canaliculata had gradually adapted to high temperature environments under long-term high temperature stress.

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Section: Discussionmentioning

confidence: 99%

Effect of long-term temperature stress on the intestinal microbiome of an invasive snail

Qian

Gao

et al. 2022

Front. Microbiol.

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“… 3 They are emerging opportunistic human pathogens that can cause host wound infections, gastrointestinal infections, and even bacteremia. 4 , 5 …”

Section: Introductionmentioning

confidence: 99%

Detection of NDM-1-Positive Aeromonas caviae from Bacteremia by Using Whole-Genome Sequencing

Tu¹,

Zhang²,

Chen³

et al. 2022

IDR

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Purpose Nosocomial infections caused by New Delhi metallo-β-lactamase (NDM)-producing bacteria are prevalent worldwide. However, such diseases caused by NDM-producing Aeromonas caviae had never been reported. Our study aimed to elucidate the genomic characteristics of NDM-1-producing A. caviae isolated from hospital patients. Methods Bacterial genomic features and possible origins were assessed by whole-genome sequencing (WGS) and phylogenetic analysis. Subsequent investigations include antimicrobial susceptibility testing and multilocus sequence typing (MLST). Results We identified here two NDM-1-producing A. caviae isolates from bacteremia. Susceptibility testing showed that two isolates were multi-drug resistant and shared a similar resistance profile and were only sensitive to amikacin and trimethoprim/sulfamethoxazole. Both A. caviae isolates carry the carbapenem resistance gene bla NDM-1 and also have antibiotic resistance genes such as β-lactams, AmpC enzymes, macrolides, aminoglycosides, and quinolones. S1-PFGE and Southern blot analysis were negative. Whole-genome sequencing and comparative analysis revealed that these two isolates shared a close relationship. Conclusion To the best of our knowledge, this work describes the first detection of non-plasmid encoded bla NDM-1 in A. caviae . The A. caviae isolated in this study has a broad drug resistance spectrum. Phenotypic and molecular analysis indicated the two isolates belong to the same clone. Routine genomic surveillance of this species is now necessary to effectively curb the further dissemination of carbapenem-resistant bacteria in the region.

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“…and cause infections including hemorrhagic septicemia, exophthalmia, ulcerative infections, fin rot, loss of scale, skin discolouration, haemorrhagic/necrotized internal organ, dropsy and red sore in carp, tilapia, perch, goldfish, catfish and salmon (Janda and Abbott 2010;Furmanek-Blaszk 2014;Pauzi et al 2020;Azzam-Sayuti et al 2021). Among them, hemorrhagic septicemia is one of the most important diseases affecting the aquaculture industry worldwide, and the genus Aeromonas, is known as an important opportunistic pathogen and responsible for hemorrhagic septicemia in carp, tilapia, perch, catfish and salmon (Janda and Abbott 2010;Hu et al 2012;Hossain et al 2014;Tekedar et al 2020;De Silva et al 2021).…”

Section: Introductionmentioning

confidence: 99%

“…Besides, A. hydrophila has known as a zoonotic pathogen and is associated with several food-borne and waterborne outbreaks (Igbinosa et al 2012;Yang et al 2018). This bacterium has been isolated from patients with traveller's diarrhoea and can cause gastroenteritis, wound infections, meningitis, peritonitis and septicemia in immunocompetent and immunocompromised individuals (Sha et al 2002;Igbinosa et al 2012;Piotrowska and Popowska 2014;De Silva et al 2021).…”

Section: Introductionmentioning

confidence: 99%

Antibiotic resistance and virulence factor gene profile of A. hydrophila isolated from carp (Cyprinidae) suspected with hemorrhagic septicemia in Gilan, Iran

Goudarztalejerdi

Yavari

Kalourazi

et al. 2022

Letters in Applied Microbiology

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Significance and Impact of the Study: Hemorrhagic septicemia is one of the most important diseases affecting the aquaculture industry, and the aeromonads are known as main causative agent of hemorrhagic septicemia in carp. This study revealed a high frequency of multi-drug resistance (MDR) and virulence factor genes (VFGs) among the Aeromonas hydrophila isolates from carp suspected with hemorrhagic septicemia, and highlighted the role of contaminated carp as reservoirs for more virulent and MDR isolates, which can represent a potential significant public health risk via seafood-borne infections. Also, the incidence of integrons in these isolates suggests the possibile role of transposable elements in the horizontal transfer of antibiotic resistance genes (ARGs) and VFGs between bacteria.

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Virulence and antimicrobial resistance potential ofAeromonasspp. associated with shellfish

Cited by 30 publications

References 81 publications

Effect of long-term temperature stress on the intestinal microbiome of an invasive snail

Effect of long-term temperature stress on the intestinal microbiome of an invasive snail

Detection of NDM-1-Positive Aeromonas caviae from Bacteremia by Using Whole-Genome Sequencing

Antibiotic resistance and virulence factor gene profile of A. hydrophila isolated from carp (Cyprinidae) suspected with hemorrhagic septicemia in Gilan, Iran

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