Antibiotic resistance of Campylobacter jejuni isolates recovered from humans with diarrhoea in Turkey

Kayman, Tuba; Abay, Seçil; Aydın, Fuat; Şahin, Orhan

doi:10.1099/jmm.0.000890

Cited by 23 publications

(22 citation statements)

References 39 publications

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“…A high rate (73.4%) of ciprofloxacin resistance among C. jejuni human isolates detected in the present study shows similarity to the limited number of previous studies carried out in Turkey (17,18). The high rate of fluoroquinolone resistance among C. jejuni chicken isolates reported in a recent study in Turkey may reflect the over-use of the antibiotic in animal husbandry, which may be responsible for the high rate of resistance among human strains detected in the present study (27).…”

Section: Discussionsupporting

confidence: 88%

“…Although numerous gyrA mutations related to the emergence of ciprofloxacin resistance have been reported in other countries, in two studies carried out in Turkey, one related with C. jejuni of human and the other of chicken isolates, all of the strains that were resistant to ciprofloxacin was determined to have C257T mutation at the codon of 86 (17,28). Parallel to these studies, in the present study, strains that were found to be resistant and susceptible to ciprofloxacin were determined to give different emissions of the reporter fluors with wild-type and mutant probes in allelic discrimination using AriaMx Real-time PCR system indicating that the system can be used accurately for the quick screening of the resistance.…”

Section: Discussionmentioning

confidence: 99%

“…More than 50% of travel‐related Campylobacter infections in the United States of America, Denmark, United Kingdom, and Norway were shown to be due to ciprofloxacin‐resistant strains . Studies related to the rate of ciprofloxacin resistance and its molecular mechanisms among Campylobacter isolates of humans in Turkey, a country that is highly attractive to tourists, are limited . It is evident that studies investigating the rate of ciprofloxacin resistance and its mechanism among Campylobacter strains in Turkey will provide valuable data about the treatment of the infection not only for Turkey but also for other countries where travel to Turkey is frequent.…”

mentioning

confidence: 99%

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Molecular and phenotypical investigation of ciprofloxacin resistance among Campylobacter jejuni strains of human origin: high prevalence of resistance in Turkey

İlktaç

Öngen

Humphrey

et al. 2019

APMIS

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Campylobacteriosis is one of the most frequently reported zoonoses worldwide. The well‐documented increase in the ciprofloxacin resistance has increased the importance of rapid detection of the resistance. The incidence of ciprofloxacin resistance was investigated using real‐time PCR. Identification of one hundred and fifty‐eight strains was performed by PCR. Minimum inhibitory concentration (MIC) of ciprofloxacin was determined by Epsilometer test. Following the confirmation of the efficiencies of singleplex real‐time PCR methods using two different probes, a cytosine to thymine point mutation at codon 86 was detected by allelic discrimination. Of the 158 strains, 114 (72.2%) were determined to be resistant to ciprofloxacin. The MIC50 and the MIC90 of ciprofloxacin were found to be 8 and ≥32 mg/L, respectively. By real‐time PCR, the presence of the mutation was confirmed in all, but one, resistant strains and the absence of the mutation was demonstrated in all, but one, susceptible strains. The rate of resistance is high among C. jejuni strains and ciprofloxacin should not be used in the treatment of such infections in Turkey. A cytosine to thymine mutation is the most frequently detected mechanism for the resistance. Real‐time PCR can be used for the quick screening of the resistance.

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Section: Discussionsupporting

confidence: 88%

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

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Molecular and phenotypical investigation of ciprofloxacin resistance among Campylobacter jejuni strains of human origin: high prevalence of resistance in Turkey

İlktaç

Öngen

Humphrey

et al. 2019

APMIS

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“…[42][43][44][45][46] The resistance to fluoroquinolones is especially a concern as in many countries, the majority of Campylobacter isolates are no longer susceptible to this class of antibiotics. 18,[47][48][49][50] In the U.S., a recent CDC report revealed a rising trend of ciprofloxacin-resistant Campylobacter for the past two decades and the resistance rate reached 29% in 2017. 9 A unique feature of fluoroquinolone resistance in Campylobacter is its continued persistence or even increased prevalence in the absence of antibiotic usage.…”

Section: Antibiotic Resistance and Alternative Antibiotic Therapymentioning

confidence: 99%

New and alternative strategies for the prevention, control, and treatment of antibiotic-resistant Campylobacter

Dai¹,

Şahin

Grover

et al. 2020

Translational Research

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Campylobacter is an enteric pathogen and a leading bacterial cause of diarrhea worldwide. It is widely distributed in food animal species and is transmitted to humans primarily through the foodborne route. While generally causing self-limited diarrhea in humans, Campylobacter may induce severe or systemic infections in immunocompromised or young/elderly patients, which often requires antibiotic therapy with the first-line antibiotics including fluoroquinolones and macrolides. Over the past decades, Campylobacter has acquired resistance to these clinically significant antibiotics, compromising the effectiveness of antibiotic treatments. To address this concern, many studies have been conducted to advance novel and alternative measures to control antibiotic-resistant Campylobacter in animal reservoirs and in the human host. Although some of these undertakings have yielded promising results, efficacious and reliable alternative approaches are yet to be developed. In this review article, we will describe Campylobacter-associated disease spectrums and current treatment options, discuss the state of antibiotic resistance and alternative therapies, and provide an evaluation of various approaches that are being developed to control Campylobacter infections in animal reservoirs and the human host.

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“…While many literature reports have been published on the development of antibiotic resistance in major Campylobacter species, particularly C. jejuni and C. coli, to antibiotics used in human and veterinary medicine (Novoa Rama et al 2018;Kayman et al 2019;Yang et al 2019), research on the antibiotic susceptibility of less common species such as C. sputorum is rather scarce. On et al (1999) reported that the C. sputorum strain they isolated from an axillary abscess was susceptible to E, AM, nalidixic acid, CN, ciprofloxacin, cefazolin and cefotaxime.…”

Section: Discussionmentioning

confidence: 99%

Species distribution, genetic diversity and antimicrobial susceptibility of Campylobacter isolates recovered from the preputial cavity of healthy rams in Turkey

Aydın

Abay

Şahin

et al. 2020

J. Appl. Microbiol.

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Aims: Campylobacter sp. are important causes of reproductive disease in ruminants worldwide. Although healthy bulls are well-known carriers for infection of cows, the role of rams as a potential source for infecting ewes is unclear. This study aimed to determine prevalence, species distribution, genetic diversity and antimicrobial susceptibility profiles of Campylobacter sp. isolated from the preputial cavity of healthy rams. Methods and Results: The material of this prospective study comprised 191 swab samples taken from the preputial cavity of healthy rams. Enrichment and membrane filtration were employed for the isolation of Campylobacter. Presumptive isolates were confirmed as Campylobacter by phenotypic and molecular tests. 16S rRNA gene sequence analysis was used for the definitive identification of the isolates at species level, and genotyping was performed using pulsed-field gel electrophoresis (PFGE). The susceptibility of the Campylobacter sp. isolates to various antibiotics was determined by the disk diffusion test. In all, 27 of the 191 (14Á13%) swab samples were found to be positive for Campylobacter sp. (28 isolates were recovered in total). Per phenotypic and genotypic analyses, one isolate was identified as Campylobacter mucosalis and the remaining 27 isolates were identified as Campylobacter sputorum bv. faecalis. The PFGE analysis of the C. sputorum biovar faecalis isolates produced 17 clusters and 24 different pulsotypes, indicating high genetic heterogeneity. All 28 isolates were found to be susceptible to all of the antibiotics tested. Conclusions: Healthy rams may be an important reservoir of different Campylobacter species in the preputium. Significance and Impact of the Study: This study demonstrated for the first time that healthy rams can carry different Campylobacter sp. including genetically diverse C. sputorum bv. faecalis and C. mucosalis in the preputial cavity. Further investigation on the potential implication of this finding on sheep reproductive health (e.g. infectious infertility, and abortion) and overall epidemiology of Campylobacter may be warranted.

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Antibiotic resistance of Campylobacter jejuni isolates recovered from humans with diarrhoea in Turkey

Cited by 23 publications

References 39 publications

Molecular and phenotypical investigation of ciprofloxacin resistance among Campylobacter jejuni strains of human origin: high prevalence of resistance in Turkey

Molecular and phenotypical investigation of ciprofloxacin resistance among Campylobacter jejuni strains of human origin: high prevalence of resistance in Turkey

New and alternative strategies for the prevention, control, and treatment of antibiotic-resistant Campylobacter

Species distribution, genetic diversity and antimicrobial susceptibility of Campylobacter isolates recovered from the preputial cavity of healthy rams in Turkey

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