New and alternative strategies for the prevention, control, and treatment of antibiotic-resistant Campylobacter

Dai, Lei; Şahin, Orhan; Grover, Madhusudan; Zhang, Qijing

doi:10.1016/j.trsl.2020.04.009

Cited by 81 publications

(75 citation statements)

References 148 publications

(163 reference statements)

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“…have been reported to be resistant to antibiotics including fluoroquinolones, beta-lactams, macrolides, and aminoglycosides [ 4 , 5 ]. Fluoroquinolones (e.g., ciprofloxacin) and macrolides (e.g., azithromycin) are the primary antibiotics used for treatment in humans, and thus resistance to these classes of drugs in Campylobacter is a significant public health concern [ 6 , 7 ]. Whereas fluoroquinolone resistance develops easily, macrolide resistance in Campylobacter is a gradual process requiring prolonged exposure to the antibiotic [ 8 ].…”

Section: Introductionmentioning

confidence: 99%

Distribution of CRISPR Types in Fluoroquinolone-Resistant Campylobacter jejuni Isolates

Adıgüzel

Goulart

et al. 2021

Pathogens

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To aid development of phage therapy against Campylobacter, we investigated the distribution of the clustered regularly interspaced short palindromic repeats (CRISPR) systems in fluoroquinolone (FQ)-resistant Campylobacter jejuni. A total of 100 FQ-resistant C. jejuni strains from different sources were analyzed by PCR and DNA sequencing to determine resistance-conferring mutation in the gyrA gene and the presence of various CRISPR systems. All but one isolate harbored 1–5 point mutations in gyrA, and the most common mutation was the Thr86Ile change. Ninety-five isolates were positive with the CRISPR PCR, and spacer sequences were found in 86 of them. Among the 292 spacer sequences identified in this study, 204 shared 93–100% nucleotide homology to Campylobacter phage D10, 44 showed 100% homology to Campylobacter phage CP39, and 3 had 100% homology with Campylobacter phage CJIE4-5. The remaining 41 spacer sequences did not match with any phages in the database. Based on the results, it was inferred that the FQ-resistant C. jejuni isolates analyzed in this study were potentially resistant to Campylobacter phages D10, CP39, and CJIE4-5 as well as some unidentified phages. These phages should be excluded from cocktails of phages that may be utilized to treat FQ-resistant Campylobacter.

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Section: Introductionmentioning

confidence: 99%

Distribution of CRISPR Types in Fluoroquinolone-Resistant Campylobacter jejuni Isolates

Adıgüzel

Goulart

et al. 2021

Pathogens

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“…In isolates obtained from animals, the same pattern of resistance occurs in both C. coli and C. jejuni isolated from fattening pigs and calves [1]. The Campylobacter genus, being both a zoonotic and enteric microorganism, has acquired several antimicrobial resistances due to its exposure to antimicrobials used in the treatment/prophylaxis of disease in food-producing animals, companion animals and humans [2].…”

Section: Introductionmentioning

confidence: 99%

Occurrence of tet(O/M/O) Mosaic Gene in Tetracycline-Resistant Campylobacter

et al. 2020

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Campylobacter is one of the most important microorganisms responsible for foodborne diseases in the EU. In this study, we investigated resistance to tetracycline in 139 Campylobacter jejuni and Campylobacter coli samples isolated from human clinical cases. From these, 110 were resistant to tetracycline, with MIC (minimal inhibitory concentration) varying in a range of 1 to >512 μg/mL, and 109 (78.4%) carried tet(O), a gene that confers resistance to tetracycline through the expression of a protein that confers protection to the ribosome. Amongst the tetracycline-resistant isolates, one C. jejuni (HCC30) was the only tet(O)-negative sample, presenting an MIC of 256 μg/mL. Instead, the mosaic gene tet(O/M/O) was found in HCC30 and, as far as we know, this is the first description of this chimeric gene originating from homologous recombination between tet(O) and tet(M). The previously described mosaic gene tet(O/32/O), also found in Campylobacter, presents a chimeric structure very similar to that of tet(O/M/O), affecting domains II and III of encoded proteins distantly related to the elongation factor G (EF-G). The tet(O/M/O) mosaic gene has been found in nucleotide databases in several genomes of Campylobacter isolated from different origins, indicating its frequent acquisition, even though it can be undetected through screening by PCR with specific tet(O) primers. In this work, we address the improvement of classical PCR to efficiently diagnose the most prevalent tetracycline resistance determinants in Campylobacter, including tet(O/M/O), which should be taken into account in the optimization of campylobacteriosis treatments.

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“…The main mechanisms of AMR include mutations in specific genes and acquiring efflux pumps [ 11 ]. For instance, the main resistance mechanism to ciprofloxacin is through target mutation in the DNA gyr A gene, along with the CmeABC efflux pump [ 4 , 12 ]; the majority (75–90%) of Campylobacter isolates worldwide have developed resistance to this important category of antibiotics [ 13 ]. The resistance to tetracycline is known to be either on a plasmid or bacterial chromosome [ 14 , 15 , 16 ].…”

Section: Introductionmentioning

confidence: 99%

Susceptibility of Campylobacter Strains to Selected Natural Products and Frontline Antibiotics

Gahamanyi

Song²,

Hyun³

et al. 2020

Antibiotics

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Campylobacter species have developed resistance to existing antibiotics. The development of alternative therapies is, therefore, a necessity. This study evaluates the susceptibility of Campylobacter strains to selected natural products (NPs) and frontline antibiotics. Two C. jejuni strains (ATCC® 33560TM and MT947450) and two C. coli strains (ATCC® 33559TM and MT947451) were used. The antimicrobial potential of the NPs, including plant extracts, essential oils, and pure phytochemicals, was evaluated by broth microdilution. The growth was measured by spectrophotometry and iodonitrotetrazolium chloride. Antibiotic resistance genes (tet(O) and gyrA) were characterized at the molecular level. The minimum inhibitory concentrations (MICs) and the minimum bactericidal concentrations (MBCs) ranged from 25 to 1600 µg/mL. Cinnamon oil, (E)-Cinnamaldehyde, clove oil, eugenol, and baicalein had the lowest MIC and MBC values (25–100 µg/mL). MT947450 and MT947451 were sensitive to erythromycin and gentamicin but resistant to quinolones and tetracycline. Mutations in gyrA and tet(O) genes from resistant strains were confirmed by sequencing. The findings show that NPs are effective against drug-sensitive and drug-resistant Campylobacter strains. The resistance to antibiotics was confirmed at phenotypic and genotypic levels. This merits further studies to decipher the action mechanisms and synergistic activities of NPs.

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New and alternative strategies for the prevention, control, and treatment of antibiotic-resistant Campylobacter

Cited by 81 publications

References 148 publications

Distribution of CRISPR Types in Fluoroquinolone-Resistant Campylobacter jejuni Isolates

Distribution of CRISPR Types in Fluoroquinolone-Resistant Campylobacter jejuni Isolates

Occurrence of tet(O/M/O) Mosaic Gene in Tetracycline-Resistant Campylobacter

Susceptibility of Campylobacter Strains to Selected Natural Products and Frontline Antibiotics

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