Antibiofilm Activity of Sodium Hypochlorite and Alkaline Tetrasodium EDTA Solutions

Solana, Carmen; Ruiz-Linares, Matilde; Baca, Pilar; Valderrama, Mariano J.; Arias-Moliz, María Teresa; Ferrer-Luque, Carmen Marı́a

doi:10.1016/j.joen.2017.06.038

Cited by 22 publications

(23 citation statements)

References 23 publications

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“…Cell viability was determined by means of the adenosine triphosphate (ATP) assay (BacTiter-Glo™, Promega, Madison, WI, USA) 18) . For the ATP assay, 100 µL of bacterial suspension was added to 100 µL BacTiter-Glow reagent in a 96-well white plate (Greiner, Monroe, NC, USA) followed by incubation at room temperature for 5 min.…”

Section: Methodsmentioning

confidence: 99%

Antibacterial and antibiofilm activity over time of GuttaFlow Bioseal and AH Plus

et al. 2019

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The sealers' antibacterial and antibiofilm activities against Enterococcus faecalis were evaluated by direct contact test (DCT) and confocal laser scanning microscopy (CLSM), respectively, after 1 day, and 1 and 4 weeks of aging. Cell viability was determined by adenosin triphosphate (ATP) assay after DCT. The parameters evaluated for the antibiofilm property were total biovolume and percentage of green cells in E.faecalis biofilms. The data from the bioluminescence ATP assay as well as the total biovolume and green percentage were analyzed by non-parametric tests, Kruskal-Wallis for global comparison and Kolmogorov-Smirnov for each two variables. Results of the DCT and CLSM for all parameters evaluated show that the antimicrobial activity of AH Plus decreased over time, whereas GuttaFlow Bioseal had an opposite property, increasing its antibacterial activity as the material aged.

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Section: Methodsmentioning

confidence: 99%

Antibacterial and antibiofilm activity over time of GuttaFlow Bioseal and AH Plus

et al. 2019

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“…This loss, which is also concentration-dependent, is responsible for the lower toxicity seen when EDTANa 4 is combined with NaOCl. Such a finding suggests an extra advantage in using this combination because the antibiofilm activity is not reduced with respect to 2.5% NaOCl alone [8].…”

Section: Discussionmentioning

confidence: 94%

“…The addition of surfactants to the preparations of NaOCl accelerated the degradation of free available chlorine [29], most likely because of the reaction between NaOCl and the surfactants that are organic compounds [30]. These combined solutions did not modify the antibiofilm activity [8], which could be explained by the action of CTR disrupting the biofilms in addition to its antimicrobial activity.…”

Section: Discussionmentioning

confidence: 99%

“…The greater viability obtained by the 20% EDTANa 4 solution (either alone or in combination with NaOCl) with respect to the 10% EDTANa 4 solution could be related to the amount of sodium ions present in the chelating agent. The exposure of organic samples, such as bovine muscle [9] or the biofilm of E. faecalis [8], to these solutions favors hydration by deposition on the surface of sodium ions in a concentration-dependent manner, which could be linked to the lower toxicity found for the 20% EDTANa 4 solution.…”

Section: Discussionmentioning

confidence: 99%

“…Combined solutions of etidronate (HEDP) and alkaline tetrasodium EDTA (EDTANa 4 ) with NaOCl maintain the proteolytic and antibacterial effects of NaOCl [7,8] as well as the ability of NaOCl to remove the smear layer [5,9]. Incorporating surfactant agents with irrigating solutions improves the disinfecting efficacy [10,11] and wetting properties of the solutions [12,13].…”

Section: Introductionmentioning

confidence: 99%

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Cytotoxic effects of alkaline tetrasodium EDTA irrigating solutions

et al. 2020

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The aim of this study is to determine the cytotoxic effects of tetrasodium ethylenediaminetetraacetic acid (EDTANa 4) when used alone or when combined with sodium hypochlorite (NaOCl), with and without the addition of cetrimide (CTR). Human pulmonary fibroblast cell line was exposed to the following irrigating solutions: group 1, 2.5% NaOCl; group 2, 10% EDTANa 4 ; group 3, 20% EDTANa 4 ; group 4, 2.5% NaOCl/5% EDTANa 4 ; group 5, 2.5% NaOCl/10% EDTANa 4 ; group 6, 2.5% NaOCl/5% EDTANa 4 /0.2% CTR; group 7, 2.5% NaOCl/10% EDTANa 4 /0.2% CTR; group 8, control, cells in Dulbecco's modified Eagle's medium. Methyl thiazol tetrazolium assay was used to determine the viability of cells after 1 and 24 h. Viability percentages were analyzed for global comparison using the Welch test followed by the Games-Howell test to determine groups with similar viability, and the Student's t test was used to compare the two times. The lowest viability was obtained with a 2.5% NaOCl solution at both time periods. The association of NaOCl with EDTANa 4 increased the cellular viability in direct relation with the concentration of the chelating agent. Globally, after 24 h of exposure, cell viability reduced. The solutions of EDTANa 4 showed moderate cytotoxic effects when compared with NaOCl alone.

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Irrigating Solutions, Devices, and Techniques

Boutsioukis

Arias-Moliz

2021

Endodontic Materials in Clinical Practice

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Antibiofilm Activity of Sodium Hypochlorite and Alkaline Tetrasodium EDTA Solutions

Cited by 22 publications

References 23 publications

Antibacterial and antibiofilm activity over time of GuttaFlow Bioseal and AH Plus

Antibacterial and antibiofilm activity over time of GuttaFlow Bioseal and AH Plus

Cytotoxic effects of alkaline tetrasodium EDTA irrigating solutions

Irrigating Solutions, Devices, and Techniques

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