Cytotoxic effects of alkaline tetrasodium EDTA irrigating solutions

Solana, Carmen; Ferrer-Luque, Carmen Marı́a; Wang, Xu; Baca, Pilar; Ruiz-Linares, Matilde; Cabrera, Teresa

doi:10.2334/josnusd.19-0234

Cited by 6 publications

(9 citation statements)

References 30 publications

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“…The cells were subsequently counted in a Neubauer chamber (Brand GmbH + CO KG, Wertheim, Germany). Adherent cells in a logarithmic growth phase were seeded in 96-well flat-bottom microtiter plates (Jet Biofil, Guangzhou, China) at a concentration of 10 4 cells/well, then incubated at 37 °C for 24 h with 5% CO 2 [ 45 ].…”

Section: Methodsmentioning

confidence: 99%

Antimicrobial Activity and Cytotoxicity of Nonsteroidal Anti-Inflammatory Drugs against Endodontic Biofilms

Ferrer-Luque

Solana

Aguado³

et al. 2023

Antibiotics

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Persistent infections have become a challenge in dentistry because of growing antibiotic resistance. Nonsteroidal anti-inflammatory drugs (NSAIDs) appear to be a therapeutic alternative to control biofilm infection. The objective of this work is to evaluate the antimicrobial activity and cytotoxicity of sodium diclofenac (DCS), ibuprofen (IBP) and ibuprofen arginine (IBP-arginine) solutions against endodontic polymicrobial biofilms. Sterile radicular dentin blocks of 4 mm × 4 mm × 0.7 mm were used as substrate to grow biofilm. The dentin blocks were submerged into solutions for 5 min. The antimicrobial activity was evaluated by means of the adenosine triphosphate (ATP) assay and confocal laser scanning microscopy (CLSM). Fibroblasts 3T3-L1 (ECACC 86052701) were used to test the cytotoxicity of irrigating solutions. The antibiofilm effects determined by the ATP assay showed that 4% IBP-arginine solution exerted the highest antibiofilm activity, followed by 4% DCS and 4% IBP, with statistical differences among groups (p < 0.001). As for CLSM, 4% DCS and 4% IBP-arginine solutions gave the lowest viable cell percentages, without significant differences between them. Cytotoxicity results at 1/10 dilution were similar for all solutions. At 1/100 dilution, a 4% DCS solution obtained the lowest cell viability for both time periods assayed, 1 h and 24 h. The IBP-arginine group showed the highest cell viability at 24 h. In this preliminary study, in terms of antibiofilm activity and cytotoxicity, a mixed 4% IBP-arginine solution gave the most promising results. NSAID solutions could be recommendable drugs for endodontic disinfection procedures.

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Section: Methodsmentioning

confidence: 99%

Antimicrobial Activity and Cytotoxicity of Nonsteroidal Anti-Inflammatory Drugs against Endodontic Biofilms

Ferrer-Luque

Solana

Aguado³

et al. 2023

Antibiotics

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“…Root canal treatment is commonly prescribed to treat the infected pulp tissue of the human tooth. [ 1 ] Essential steps of the root canal treatment procedure involve removing the pulp, followed by sterilizing the root canal space using bactericidal chemicals (sodium hypochlorite bleach, [ 2 ] hydrogen peroxide, ethylenediaminetetraacetic acid (EDTA), [ 3 ] etc.) for mitigating the microbial load.…”

Section: Introductionmentioning

confidence: 99%

Mobile Nanobots for Prevention of Root Canal Treatment Failure

Dasgupta

Saini

et al. 2022

Adv Healthcare Materials

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Millions of root canal treatments fail worldwide due to remnant bacteria deep in the dentinal tubules located within the dentine tissue of human teeth. The complex and narrow geometry of the tubules renders current techniques relying on passive diffusion of antibacterial agents ineffective. Here, the potential of actively maneuvered nanobots is investigated to disinfect dentinal tubules, which can be incorporated during a standard root canal procedure. It is demonstrated that magnetically driven nanobots can reach the depths of the tubules not possible with current clinical practices. Subtle alterations of the magnetic drive allow both deep implantations of the nanobots isotopically distributed throughout the dentine and spatially controlled recovery from selected regions, further supported by numerical simulations. Finally, the integration of bactericidal therapeutic modality with the nanobots is demonstrated, thereby validating the tremendous potential of nanobots in dentistry and nanomedicine in general.

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“…Root canal treatment procedure is commonly prescribed to treat the infected pulp tissue of the human tooth [1]. Essential steps of the root canal treatment procedure involve careful removal of the pulp, followed by sterilization of the root canal space using harsh chemicals (sodium hypochlorite bleach [2], hydrogen peroxide, EDTA [3] etc.) for mitigating the microbial load.…”

Section: Introductionmentioning

confidence: 99%

Implantation and Retrieval of Magnetic Nanobots for Treatment of Endodontic Re-Infection in Human Dentine

Dasgupta

Peddi²,

Saini³

et al. 2021

Preprint

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<div> <div> <div> <p>More than 10% of root canal treatments undergo failure worldwide due to remnant bacteria deep in the dentinal tubules located within the dentine tissue of human teeth. Owing to the complex and narrow geometry of the tubules, current techniques relying on passive diffusion of anti-bacterial agents are inadequate. Here, we present a new treatment method using actively maneuvered nanobots, which can be incorporated during standard root canal procedure. Our technique will enable dentists to execute procedures inside the dentine not yet possible by current state of the art. We demonstrate that magnetically driven nanobots can reach the depths of the tubules up to hundred times faster than current clinical practices. Subtle modifications of the magnetic drive allowed deep implantation of the nanobots isotopically distributed throughout the dentine, along with spatially controlled retrieval from selected areas. Finally, we demonstrate the integration of bactericidal therapeutic modality with the nanobots, thereby validating the tremendous potential of nanobots in dentistry, and nanomedicine in general. </p> </div> </div> </div>

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Cytotoxic effects of alkaline tetrasodium EDTA irrigating solutions

Cited by 6 publications

References 30 publications

Antimicrobial Activity and Cytotoxicity of Nonsteroidal Anti-Inflammatory Drugs against Endodontic Biofilms

Antimicrobial Activity and Cytotoxicity of Nonsteroidal Anti-Inflammatory Drugs against Endodontic Biofilms

Mobile Nanobots for Prevention of Root Canal Treatment Failure

Implantation and Retrieval of Magnetic Nanobots for Treatment of Endodontic Re-Infection in Human Dentine

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